PMID- 16497695
OWN - NLM
STAT- MEDLINE
DCOM- 20060926
LR  - 20220310
IS  - 0268-1161 (Print)
IS  - 0268-1161 (Linking)
VI  - 21
IP  - 7
DP  - 2006 Jul
TI  - Chromosome constitution and apoptosis of immature germ cells present in sperm of 
      two 47,XYY infertile males.
PG  - 1749-58
AB  - BACKGROUND: In order to assess sperm alterations observed in some XYY males, we 
      analysed the chromosome constitution as well as apoptosis expression in germ 
      cells from two oligozoospermic males with high count of immature germ cells in 
      their semen. METHODS: Sex chromosome number and distribution were assessed at 
      pachytene stage by fluorescence in situ hybridization (FISH). Immature germ cells 
      and spermatozoa were examined by FISH and TdT (terminal deoxynucleotidyl 
      transferase)-mediated dUDP nick-end (TUNEL) assay, combined with 
      immunocytochemistry using the proacrosin-specific monoclonal antibody (mAb 4D4). 
      RESULTS: For patients 1 and 2, two Y chromosomes were present in respectively 
      60.0 and 39.6% of pachytenes. The three sex chromosomes were always in close 
      proximity and partially or totally condensed in a sex body. XYY spermatocytes I 
      escape the pachytene checkpoint and achieve meiosis. Nevertheless, nuclear 
      division and/or cytokinesis were often impaired during meiosis leading to diploid 
      (mainly 47,XYY cells) and tetraploid (94,XXYYYY) meiocytes. The presence of 
      binucleated (23,Y)(24,XY) immature germ cells resulting from cytokinesis failure 
      agree with a preferential segregation of the two Y chromosomes during meiosis I. 
      In addition, 69.6% (patient 1) and 53.12% (patient 2) of post-reductional round 
      germ cells were XY. However, high level of apoptotic round germ cells (94.9% for 
      patient 1 and 93.3% for patient 2) was detected and may explain the moderate 
      increase of hyperhaploid XY spermatozoa. Segregation errors also occurred in the 
      XY cell line responsible for disomic 18 and X, as well as 46,XY diploid 
      spermatozoa. CONCLUSIONS: Our data are in agreement with the persistence of the 
      extra Y chromosome during meiosis in XYY oligozoospermic males responsible for 
      spermatogenesis impairment and a probable elimination via apoptosis of most XYY 
      germ cells not solely during but also after meiosis.
FAU - Milazzo, J P
AU  - Milazzo JP
AD  - Reproductive Biology Laboratory - CECOS, Rouen University Hospital, Rouen, 
      France.
FAU - Rives, N
AU  - Rives N
FAU - Mousset-Simeon, N
AU  - Mousset-Simeon N
FAU - Mace, B
AU  - Mace B
LA  - eng
PT  - Journal Article
DEP - 20060223
PL  - England
TA  - Hum Reprod
JT  - Human reproduction (Oxford, England)
JID - 8701199
SB  - IM
MH  - Adult
MH  - Apoptosis/*physiology
MH  - Chromosomes, Human, Y/*ultrastructure
MH  - DNA Fragmentation
MH  - Germ Cells/*cytology
MH  - Humans
MH  - In Situ Hybridization, Fluorescence
MH  - In Situ Nick-End Labeling
MH  - Male
MH  - Meiosis/physiology
MH  - Oligospermia/*genetics
MH  - Spermatozoa/ultrastructure
MH  - XYY Karyotype/*genetics
EDAT- 2006/02/25 09:00
MHDA- 2006/09/27 09:00
CRDT- 2006/02/25 09:00
PHST- 2006/02/25 09:00 [pubmed]
PHST- 2006/09/27 09:00 [medline]
PHST- 2006/02/25 09:00 [entrez]
AID - del051 [pii]
AID - 10.1093/humrep/del051 [doi]
PST - ppublish
SO  - Hum Reprod. 2006 Jul;21(7):1749-58. doi: 10.1093/humrep/del051. Epub 2006 Feb 23.