PMID- 16498264
OWN - NLM
STAT- MEDLINE
DCOM- 20060605
LR  - 20211203
IS  - 1018-2438 (Print)
IS  - 1018-2438 (Linking)
VI  - 139
IP  - 4
DP  - 2006
TI  - Regulation of GATA-3, c-maf and T-bet mRNA expression in bronchoalveolar lavage 
      cells and bronchial biopsies after segmental allergen challenge.
PG  - 306-16
AB  - BACKGROUND: GATA-3 (GATA binding protein 3) and the proto-oncogene c-maf are 
      Th2-regulating transcription factors that control the expression of interleukin 
      (IL)-4 and IL-5, while T-bet (T-box expressed in T cells) is a Th1-specific 
      transcription factor that controls the expression of interferon (IFN-gamma). 
      Allergen provocation in asthmatics induces a Th2-dominated cytokine profile, but 
      so far it is unknown whether the skewed cytokine expression is reflected by the 
      expression of the respective transcriptional regulators. OBJECTIVE: The aim of 
      this study was to determine the regulation of Th1- and Th2-specific transcription 
      factors and cytokines in 10 atopic subjects with mild asthma and 5 nonatopic 
      healthy controls at baseline and after segmental sham and allergen challenge. 
      METHODS: The mRNA expression of GATA-3, c-maf and T-bet was determined by 
      real-time polymerase chain reaction in bronchoalveolar lavage (BAL) cells and 
      bronchial biopsies. The percentage of IL-4+, IL-5+ and IFN-gamma+ BAL T cells was 
      determined by flow cytometry, and BAL levels of these cytokines were measured by 
      ELISA. RESULTS: In BAL cells of asthmatics, the mRNA expression of all 
      transcription factors was increased after allergen challenge. In bronchial 
      biopsies, the basal expression of GATA-3 was increased in asthmatics compared to 
      healthy controls but decreased after allergen challenge. Compared to sham 
      challenge, the percentage of IL-5+/CD4+ BAL T cells was increased after allergen 
      challenge in asthmatics while the percentage of IFN-gamma+/CD4+ and 
      IFN-gamma+/CD8+ T cells was decreased. Expression of c-maf mRNA in BAL cells 
      correlated with IL-4+/CD4+ BAL cells and BAL IL-5 levels. CONCLUSIONS: Segmental 
      allergen challenge in asthmatics leads to increased GATA-3, c-maf and T-bet 
      expression in BAL cells but not in bronchial biopsies.
CI  - Copyright (c) 2006 S. Karger AG, Basel.
FAU - Erpenbeck, Veit J
AU  - Erpenbeck VJ
AD  - Fraunhofer Institute of Toxicology and Experimental Medicine, Hannover, Germany. 
      erpenbeck@item.fraunhofer.de
FAU - Hagenberg, Andreas
AU  - Hagenberg A
FAU - Krentel, Harald
AU  - Krentel H
FAU - Discher, Marc
AU  - Discher M
FAU - Braun, Armin
AU  - Braun A
FAU - Hohlfeld, Jens M
AU  - Hohlfeld JM
FAU - Krug, Norbert
AU  - Krug N
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20060222
PL  - Switzerland
TA  - Int Arch Allergy Immunol
JT  - International archives of allergy and immunology
JID - 9211652
RN  - 0 (Allergens)
RN  - 0 (GATA3 Transcription Factor)
RN  - 0 (IL4 protein, human)
RN  - 0 (IL5 protein, human)
RN  - 0 (Interleukin-5)
RN  - 0 (MAS1 protein, human)
RN  - 0 (Proto-Oncogene Mas)
RN  - 0 (Proto-Oncogene Proteins c-maf)
RN  - 0 (RNA, Messenger)
RN  - 0 (T-Box Domain Proteins)
RN  - 0 (T-box transcription factor TBX21)
RN  - 0 (Transcription Factors)
RN  - 207137-56-2 (Interleukin-4)
RN  - 82115-62-6 (Interferon-gamma)
SB  - IM
MH  - Adolescent
MH  - Adult
MH  - Allergens/administration & dosage/*immunology
MH  - Asthma/*genetics/*immunology/metabolism
MH  - Biopsy
MH  - Bronchi/immunology/*metabolism
MH  - Bronchoalveolar Lavage Fluid/cytology
MH  - Female
MH  - GATA3 Transcription Factor/*genetics
MH  - *Gene Expression Regulation
MH  - Humans
MH  - Interferon-gamma/metabolism
MH  - Interleukin-4/metabolism
MH  - Interleukin-5/metabolism
MH  - Lymphocyte Count
MH  - Male
MH  - Proto-Oncogene Mas
MH  - Proto-Oncogene Proteins c-maf/*genetics
MH  - RNA, Messenger
MH  - T-Box Domain Proteins
MH  - T-Lymphocyte Subsets/immunology
MH  - Transcription Factors/*genetics
EDAT- 2006/02/25 09:00
MHDA- 2006/06/06 09:00
CRDT- 2006/02/25 09:00
PHST- 2005/07/25 00:00 [received]
PHST- 2005/12/20 00:00 [accepted]
PHST- 2006/02/25 09:00 [pubmed]
PHST- 2006/06/06 09:00 [medline]
PHST- 2006/02/25 09:00 [entrez]
AID - 91602 [pii]
AID - 10.1159/000091602 [doi]
PST - ppublish
SO  - Int Arch Allergy Immunol. 2006;139(4):306-16. doi: 10.1159/000091602. Epub 2006 
      Feb 22.