PMID- 16500223
OWN - NLM
STAT- MEDLINE
DCOM- 20060615
LR  - 20220309
IS  - 0099-2399 (Print)
IS  - 0099-2399 (Linking)
VI  - 32
IP  - 3
DP  - 2006 Mar
TI  - Interleukin-1 alpha alters the expression of matrix metalloproteinases and 
      collagen degradation by pulp fibroblasts.
PG  - 186-92
AB  - Matrix metalloproteinases (MMPs) and the tissue inhibitors of MMPs (TIMPs) have 
      been suggested to play a role in dental pulp destruction. This study examined the 
      effects of interleukin (IL)-1 alpha on pulp fibroblasts. The ability of these 
      cells to degrade collagen was determined with or without IL-1 alpha utilizing a 
      cell-mediated collagen degradation assay. Reverse transcriptase-polymerase chain 
      reaction was utilized to examine the mRNA expression of multiple MMPs and TIMPs 
      with and without IL-1 alpha, while Western blot analyses and zymography were 
      utilized to examine their protein expression. The collagen degradation mediated 
      by these cells was stimulated by IL-1 alpha and inhibited by MMP inhibitors. IL-1 
      alpha increased the mRNA expression of MMP-1 and MMP-3, as well as induced MMP-7. 
      Western blot analyses confirmed these results. IL-1 alpha increased the secreted 
      protein level of TIMP-1, while only slightly affected the level of TIMP-2. These 
      results suggest that IL-1 alpha can induce pulp destruction by differentially 
      regulating MMPs and TIMPs.
FAU - Wisithphrom, Kessiri
AU  - Wisithphrom K
AD  - Department of Oral Biology, Indiana University School of Dentistry, Indianapolis, 
      Indiana 46202, USA.
FAU - Murray, Peter E
AU  - Murray PE
FAU - Windsor, L Jack
AU  - Windsor LJ
LA  - eng
GR  - AR44701/AR/NIAMS NIH HHS/United States
PT  - Journal Article
PT  - Research Support, N.I.H., Extramural
PL  - United States
TA  - J Endod
JT  - Journal of endodontics
JID - 7511484
RN  - 0 (Culture Media, Conditioned)
RN  - 0 (Interleukin-1)
RN  - 0 (Tissue Inhibitor of Metalloproteinases)
RN  - 0 (Vimentin)
RN  - 9007-34-5 (Collagen)
RN  - EC 3.4.24.- (Matrix Metalloproteinases)
MH  - Adult
MH  - Blotting, Western
MH  - Cells, Cultured
MH  - Collagen/*metabolism
MH  - Culture Media, Conditioned/pharmacology
MH  - Dental Pulp/cytology/*enzymology
MH  - Electrophoresis, Polyacrylamide Gel
MH  - Fibroblasts/drug effects/enzymology
MH  - Gene Expression/drug effects
MH  - Humans
MH  - Immunoenzyme Techniques
MH  - Interleukin-1/*pharmacology
MH  - Matrix Metalloproteinases/*biosynthesis
MH  - Reverse Transcriptase Polymerase Chain Reaction
MH  - Tissue Inhibitor of Metalloproteinases/biosynthesis
MH  - Vimentin/biosynthesis
EDAT- 2006/02/28 09:00
MHDA- 2006/06/16 09:00
CRDT- 2006/02/28 09:00
PHST- 2006/02/28 09:00 [pubmed]
PHST- 2006/06/16 09:00 [medline]
PHST- 2006/02/28 09:00 [entrez]
AID - S0099-2399(05)00099-3 [pii]
AID - 10.1016/j.joen.2005.10.055 [doi]
PST - ppublish
SO  - J Endod. 2006 Mar;32(3):186-92. doi: 10.1016/j.joen.2005.10.055.