PMID- 16500333
OWN - NLM
STAT- MEDLINE
DCOM- 20060321
LR  - 20061115
IS  - 1556-5653 (Electronic)
IS  - 0015-0282 (Linking)
VI  - 85
IP  - 3
DP  - 2006 Mar
TI  - Analysis of early meiotic events and aneuploidy in nonobstructive azoospermic 
      men: a preliminary report.
PG  - 646-52
AB  - OBJECTIVE: To study the events of spermatogenesis in azoospermic men by examining 
      meiosis I spermatocytes and postmeiotic spermatozoa. DESIGN: A preliminary 
      analysis of synaptonemal complex (SC), recombination, and chromosomal 
      constitution (meiotic and postmeiotic) in testicular tissue. SETTING: Academic 
      research environment. PATIENT(S): Three men with nonobstructive azoospermia and 
      one fertile man (vasectomy reversal: control) who underwent testicular sperm 
      extraction in preparation for intracytoplasmic sperm injection (ICSI). 
      INTERVENTION(S): Testicular tissue specimens were processed by immunofluorescence 
      analysis with antibodies against proteins associated with SC and recombination 
      events. Fluorescence in situ hybridization (FISH) for chromosomes X, Y, and 18 
      was done on spermatocytes in prophase I and on postmeiotic spermatozoa. MAIN 
      OUTCOME MEASURE(S): SC formation and recombination in meiosis I, aneuploidy. 
      RESULT(S): The number of autosomal recombination foci in each patient was not 
      statistically significantly different from control. The frequencies of XY 
      bivalents with at least one recombination focus were statistically similar in the 
      patients and control (74.2% vs. 82.6%, respectively). All observed cells in 
      pachytene had normal XY constitutions. In spite of this, the rate of 
      sex-chromosome aneuploidy in spermatozoa was statistically significantly higher 
      in the patients compared with the control (1.89% vs. 0.83%). CONCLUSION(S): The 
      combination of immunocytologic technology with FISH can add a level of precision 
      in etiologic investigations of severe male factor infertility: men can have 
      normal pairing and recombination but still yield aneuploid spermatozoa.
FAU - Ma, Sai
AU  - Ma S
AD  - Department of Obstetrics and Gynaecology, University of British Columbia, 
      Vancouver, British Columbia, Canada. sai@interchange.ubc.ca
FAU - Arsovska, Snezana
AU  - Arsovska S
FAU - Moens, Peter
AU  - Moens P
FAU - Nigro, Mark
AU  - Nigro M
FAU - Chow, Victor
AU  - Chow V
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Fertil Steril
JT  - Fertility and sterility
JID - 0372772
SB  - IM
MH  - Adult
MH  - *Aneuploidy
MH  - Case-Control Studies
MH  - Chromosome Pairing
MH  - Chromosomes, Human, Pair 18
MH  - Chromosomes, Human, X
MH  - Chromosomes, Human, Y
MH  - Fluorescent Antibody Technique
MH  - Gene Frequency
MH  - Humans
MH  - In Situ Hybridization, Fluorescence
MH  - Male
MH  - *Meiosis
MH  - Middle Aged
MH  - Oligospermia/*genetics/*pathology/physiopathology
MH  - Recombination, Genetic
MH  - Spermatocytes/pathology
MH  - Spermatogenesis
MH  - Spermatozoa/pathology
MH  - Synaptonemal Complex/genetics
EDAT- 2006/02/28 09:00
MHDA- 2006/03/22 09:00
CRDT- 2006/02/28 09:00
PHST- 2005/01/05 00:00 [received]
PHST- 2005/08/21 00:00 [revised]
PHST- 2005/08/21 00:00 [accepted]
PHST- 2006/02/28 09:00 [pubmed]
PHST- 2006/03/22 09:00 [medline]
PHST- 2006/02/28 09:00 [entrez]
AID - S0015-0282(05)04013-6 [pii]
AID - 10.1016/j.fertnstert.2005.08.055 [doi]
PST - ppublish
SO  - Fertil Steril. 2006 Mar;85(3):646-52. doi: 10.1016/j.fertnstert.2005.08.055.