PMID- 16501050
OWN - NLM
STAT- MEDLINE
DCOM- 20060626
LR  - 20161128
IS  - 0741-5400 (Print)
IS  - 0741-5400 (Linking)
VI  - 79
IP  - 5
DP  - 2006 May
TI  - Coupling of C3bi to IgG inhibits the tyrosine phosphorylation signaling cascade 
      downstream Syk and reduces cytokine induction in monocytes.
PG  - 1073-82
AB  - The effect of coupling C3bi to immunoglobulin G (IgG) immune complexes (IC) on 
      their ability to produce protein tyrosine phosphorylation and activation of the 
      mitogen-activated protein kinase (MAPK) and the Akt/protein kinase B (PKB) routes 
      was assessed in human monocytes. Cross-linking Fc receptors for IgG activated the 
      protein tyrosine kinase Syk, phospholipases Cgamma1 and Cgamma2, the MAPK 
      cascade, and the Akt/PKB route. Linkage of C3bi to the gamma-chain of IgG 
      produced a decrease of the protein bands displaying tyrosine phosphorylation, 
      whereas the MAPK cascades and the Akt/PKB route remained almost unaffected. 
      Zymosan particles, which because of their beta-glucan content mimic the effect of 
      fungi, produced a limited increase of tyrosine-phosphorylated protein bands, 
      whereas treatment of zymosan under conditions adequate for C3bi coating increased 
      its ability to induce protein tyrosine phosphorylation. Noteworthy, this was also 
      observed under conditions where other components of serum might be bound by 
      zymosan particles, for instance, serum IgG, thereby suggesting their potential 
      involvement in Syk activation. The induction of cytokines showed a changing 
      pattern consistent with the changes observed in the signaling pathways. IC 
      induced monocyte chemoattractant protein-1 (MCP-1)/CC chemokine ligand 2 (CCL2), 
      interleukin (IL)-1beta, and eotaxin-2/CCL24, which were not observed with 
      C3bi-coated IC. Zymosan induced the expression of tumor necrosis factor alpha 
      (TNF-alpha), TNF-beta, IL-10, IL-6, and MCP-2/CCL8, whereas the cytokine 
      signature of C3bi-coated zymosan also included interferon-inducible protein 
      10/CXC chemokine ligand 10, platelet-derived growth factor-BB, and I-309/CCL1. 
      Taken together, these findings indicate that C3bi targets the phagocytic cargo, 
      and engagement or diversion of the Syk route determines the phagocyte response.
FAU - Trinidad, Antonio Garcia
AU  - Trinidad AG
AD  - Instituto de Biologia y Genetica Molecular, Consejo Superior de Investigaciones 
      Cientificas and Universidad de Valladolid, Spain.
FAU - de la Puerta, Maria Luisa
AU  - de la Puerta ML
FAU - Fernandez, Nieves
AU  - Fernandez N
FAU - Bayon, Yolanda
AU  - Bayon Y
FAU - Crespo, Mariano Sanchez
AU  - Crespo MS
FAU - Alonso, Andres
AU  - Alonso A
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20060224
PL  - England
TA  - J Leukoc Biol
JT  - Journal of leukocyte biology
JID - 8405628
RN  - 0 (Cytokines)
RN  - 0 (Immunoglobulin G)
RN  - 0 (Intracellular Signaling Peptides and Proteins)
RN  - 0 (Macromolecular Substances)
RN  - 0 (beta-Glucans)
RN  - 42HK56048U (Tyrosine)
RN  - 80295-43-8 (Complement C3b)
RN  - 9010-72-4 (Zymosan)
RN  - EC 2.7.10.1 (Protein-Tyrosine Kinases)
RN  - EC 2.7.10.2 (SYK protein, human)
RN  - EC 2.7.10.2 (Syk Kinase)
RN  - EC 2.7.11.1 (Oncogene Protein v-akt)
RN  - EC 3.1.4.3 (Phospholipase C gamma)
SB  - IM
MH  - Cell Line
MH  - Complement C3b/*immunology/metabolism
MH  - Cytokines/drug effects/*metabolism
MH  - Down-Regulation/drug effects/immunology
MH  - Feedback, Physiological/immunology
MH  - Humans
MH  - Immunoglobulin G/*metabolism
MH  - Intracellular Signaling Peptides and Proteins/*immunology/metabolism
MH  - MAP Kinase Signaling System/drug effects/physiology
MH  - Macromolecular Substances/immunology/metabolism
MH  - Monocytes/drug effects/*immunology/metabolism
MH  - Oncogene Protein v-akt/metabolism
MH  - Phagocytosis/drug effects/*immunology
MH  - Phospholipase C gamma/metabolism
MH  - Phosphorylation/drug effects
MH  - Protein-Tyrosine Kinases/*immunology/metabolism
MH  - Signal Transduction/drug effects/immunology
MH  - Syk Kinase
MH  - Tyrosine/metabolism
MH  - Zymosan/pharmacology
MH  - beta-Glucans/pharmacology
EDAT- 2006/02/28 09:00
MHDA- 2006/06/27 09:00
CRDT- 2006/02/28 09:00
PHST- 2006/02/28 09:00 [pubmed]
PHST- 2006/06/27 09:00 [medline]
PHST- 2006/02/28 09:00 [entrez]
AID - jlb.1205701 [pii]
AID - 10.1189/jlb.1205701 [doi]
PST - ppublish
SO  - J Leukoc Biol. 2006 May;79(5):1073-82. doi: 10.1189/jlb.1205701. Epub 2006 Feb 
      24.