PMID- 16501052
OWN - NLM
STAT- MEDLINE
DCOM- 20060626
LR  - 20171116
IS  - 0741-5400 (Print)
IS  - 0741-5400 (Linking)
VI  - 79
IP  - 5
DP  - 2006 May
TI  - Polymorphonuclear neutrophils deliver activation signals and antigenic molecules 
      to dendritic cells: a new link between leukocytes upstream of T lymphocytes.
PG  - 977-88
AB  - Polymorphonuclear neutrophils (PMNs) are rapidly recruited to tissues upon injury 
      or infection. There, they can encounter local and/or recruited immature dendritic 
      cells (iDCs), a colocalization that could promote at least transient interactions 
      and mutually influence the two leukocyte populations. Using human live blood PMNs 
      and monocyte-derived iDCs, we examined if these leukocytes actually interacted 
      and whether this influenced DC function. Indeed, coculture with live but not 
      apoptotic PMNs led to up-regulation of membrane CD40, CD86, and human leukocyte 
      antigen (HLA)-DR on DCs. Whereas CD40 up-regulation was dependent on soluble 
      factors released by PMNs, as determined in cultures conducted in different 
      chambers, cell contact was necessary for CD86 and HLA-DR up-regulation, a process 
      that was inhibited by anti-CD18 antibodies, indicating that CD18 ligation was 
      required. We also found that via a cell contact-dependent mechanism, DCs acquired 
      Candida albicans-derived antigens from live as well as from apoptotic PMNs and 
      could thus elicit antigen-specific T lymphocyte responses. Altogether, our data 
      demonstrate the occurrence of cross-talk between human PMNs and DCs and provide 
      new insights into the immune processes occurring upstream of the interactions 
      between DCs and T lymphocytes.
FAU - Megiovanni, Anna Maria
AU  - Megiovanni AM
AD  - Unite Mixte de Recherche 7151 Centre National de la Recherche 
      Scientifique-Universite Paris, France.
FAU - Sanchez, Francoise
AU  - Sanchez F
FAU - Robledo-Sarmiento, Macarena
AU  - Robledo-Sarmiento M
FAU - Morel, Celine
AU  - Morel C
FAU - Gluckman, Jean Claude
AU  - Gluckman JC
FAU - Boudaly, Sarah
AU  - Boudaly S
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20060224
PL  - England
TA  - J Leukoc Biol
JT  - Journal of leukocyte biology
JID - 8405628
RN  - 0 (Antigens, Surface)
RN  - 0 (B7-2 Antigen)
RN  - 0 (CD18 Antigens)
RN  - 0 (CD40 Antigens)
RN  - 0 (HLA-DR Antigens)
SB  - IM
MH  - Antigen Presentation/*immunology
MH  - Antigens, Surface/immunology
MH  - B7-2 Antigen/immunology
MH  - CD18 Antigens/immunology
MH  - CD40 Antigens/immunology
MH  - Candida albicans/immunology
MH  - Candidiasis/immunology
MH  - Cell Communication/*immunology
MH  - Cells, Cultured
MH  - Coculture Techniques
MH  - Dendritic Cells/*immunology
MH  - HLA-DR Antigens/immunology
MH  - Humans
MH  - Immunity, Cellular/immunology
MH  - Immunophenotyping
MH  - Ligation
MH  - Lymphocyte Activation/*immunology
MH  - Neutrophils/*immunology
MH  - Phagocytosis/immunology
MH  - Signal Transduction/*immunology
MH  - T-Lymphocytes/immunology
MH  - Up-Regulation/immunology
EDAT- 2006/02/28 09:00
MHDA- 2006/06/27 09:00
CRDT- 2006/02/28 09:00
PHST- 2006/02/28 09:00 [pubmed]
PHST- 2006/06/27 09:00 [medline]
PHST- 2006/02/28 09:00 [entrez]
AID - jlb.0905526 [pii]
AID - 10.1189/jlb.0905526 [doi]
PST - ppublish
SO  - J Leukoc Biol. 2006 May;79(5):977-88. doi: 10.1189/jlb.0905526. Epub 2006 Feb 24.