PMID- 16501627
OWN - NLM
STAT- MEDLINE
DCOM- 20061108
LR  - 20131121
IS  - 1743-4297 (Print)
IS  - 1743-4297 (Linking)
VI  - 3 Suppl 1
DP  - 2006 Mar
TI  - Experimental models for cardiac regeneration.
PG  - S29-32
AB  - Simple ex vivo or in vitro models are most useful for testing putative cell 
      therapy protocols, as they allow quick and controlled screening of variants and 
      possible improvements. We discuss here three different models: coculture of 
      precursors of human bone marrow cells (BMCs) with mouse heart slices bearing a 
      cryogenic lesion; coculture of human BMCs and rat cardiomyocytes separated by a 
      porous membrane that allows passage of soluble substances but prevents migration 
      of nuclear material; and injection of human BMCs in developing chick heart 
      bearing burn lesions. Our results indicate that the damaged areas express 
      specific genes such as MPC1 and SDF1, and that some human BMCs migrate and graft 
      near the lesion, where they can originate cells with a cardiac phenotype that 
      produce human cardiac proteins. The frequency of this transformation is, however, 
      very low. Understanding the factors that determine and regulate nuclear 
      reprogramming and transdifferentiation would be crucial to appraising the 
      contribution of these phenomena to cardiac regeneration and, eventually, to 
      modulating them with therapeutic intent.
FAU - Sanchez, Ana
AU  - Sanchez A
AD  - Instituto de Biologia y Genetica Molecular (IBGM), Facultad de Medicina, 
      Universidad de Valladolid, Valladolid, Spain. asanchez@ibgm.uva.es
FAU - Fernandez, Maria Eugenia
AU  - Fernandez ME
FAU - Rodriguez, Arancha
AU  - Rodriguez A
FAU - Fernandez, Jesus
AU  - Fernandez J
FAU - Torre-Perez, Nuria
AU  - Torre-Perez N
FAU - Hurle, Juan M
AU  - Hurle JM
FAU - Garcia-Sancho, Javier
AU  - Garcia-Sancho J
LA  - eng
PT  - Comparative Study
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - England
TA  - Nat Clin Pract Cardiovasc Med
JT  - Nature clinical practice. Cardiovascular medicine
JID - 101226507
RN  - 0 (CXCL12 protein, human)
RN  - 0 (Chemokine CCL2)
RN  - 0 (Chemokine CXCL12)
RN  - 0 (Chemokines, CXC)
RN  - 0 (Cxcl12 protein, mouse)
RN  - 0 (RNA, Messenger)
RN  - EC 3.6.4.1 (Myosin Heavy Chains)
SB  - IM
MH  - Animals
MH  - Bone Marrow Cells/cytology/metabolism
MH  - Bone Marrow Transplantation
MH  - Cell Differentiation
MH  - Cell Movement
MH  - Cell Proliferation
MH  - Cells, Cultured
MH  - Chemokine CCL2
MH  - Chemokine CXCL12
MH  - Chemokines, CXC/metabolism
MH  - Chick Embryo
MH  - Coculture Techniques
MH  - Heart/embryology/*physiology
MH  - Humans
MH  - Mice
MH  - Myocardium/metabolism/pathology
MH  - Myocytes, Cardiac/metabolism
MH  - Myosin Heavy Chains/genetics/metabolism
MH  - Organ Culture Techniques
MH  - RNA, Messenger/metabolism
MH  - Rats
MH  - *Regeneration
EDAT- 2006/02/28 09:00
MHDA- 2006/11/10 09:00
CRDT- 2006/02/28 09:00
PHST- 2005/09/07 00:00 [received]
PHST- 2005/10/25 00:00 [accepted]
PHST- 2006/02/28 09:00 [pubmed]
PHST- 2006/11/10 09:00 [medline]
PHST- 2006/02/28 09:00 [entrez]
AID - ncpcardio0458 [pii]
AID - 10.1038/ncpcardio0458 [doi]
PST - ppublish
SO  - Nat Clin Pract Cardiovasc Med. 2006 Mar;3 Suppl 1:S29-32. doi: 
      10.1038/ncpcardio0458.