PMID- 1650261
OWN - NLM
STAT- MEDLINE
DCOM- 19910904
LR  - 20231120
IS  - 0006-4971 (Print)
IS  - 0006-4971 (Linking)
VI  - 78
IP  - 3
DP  - 1991 Aug 1
TI  - Engagement of interleukin-7 receptor stimulates tyrosine phosphorylation, 
      phosphoinositide turnover, and clonal proliferation of human T-lineage acute 
      lymphoblastic leukemia cells.
PG  - 564-70
AB  - The purposes of this study were to examine the biologic effects of the engagement 
      of the interleukin-7 receptor (IL-7R) with recombinant human interleukin-7 
      (rhIL-7) in immunophenotypically distinct T-lineage acute lymphoblastic leukemia 
      (ALL) blasts and to elucidate the biochemical nature of the IL-7R-linked 
      transmembrane signal in rhIL-7-responsive T-lineage ALL blast populations. In the 
      absence of costimulants, rhIL-7 stimulated the in vitro proliferation and colony 
      formation of freshly isolated leukemic blasts from six to eight T-lineage ALL 
      patients with a mean plating efficiency of 196 +/- 53 (background subtracted) 
      colonies/10(5) blasts plated. Stimulation of T-lineage ALL blasts with rhIL-7 
      resulted in markedly enhanced tyrosine phosphorylation of six distinct 
      phosphoproteins with molecular weights of 57, 72, 98, 123, 150, and 190 Kd, and 
      induced a rapid increase in the production of inositol-1,4,5-trisphosphate 
      (Ins-1,4,5-P3), which was inhibitable by the tyrosine-specific protein kinase 
      inhibitor genistein, but not by the serine/threonine-specific protein kinase C 
      inhibitor H7. Similarly, rhIL-7 stimulated Ins-1,4,5-P3 production in CEM-1.3 
      T-lineage ALL cells and this stimulation was inhibitable by the tyrosine-specific 
      protein kinase inhibitors genistein and herbimycin A, but not by H-7. Thus, the 
      transmembrane signal triggered by engagement of the IL-7R is intimately linked to 
      a functional tyrosine-specific protein kinase pathway and stimulates the 
      phosphoinositide (PI) turnover and proliferation of T-lineage ALL blasts. The 
      presented data confirm and extend previous studies on the expression of 
      functional IL-7R on T-lineage ALL blasts and support the hypothesis that IL-7 may 
      play an important regulatory role in the biology of T-lineage ALL.
FAU - Dibirdik, I
AU  - Dibirdik I
AD  - Department of Therapeutic Radiology-Radiation Oncology, University of Minnesota 
      Health Sciences Center, Minneapolis 55455.
FAU - Langlie, M C
AU  - Langlie MC
FAU - Ledbetter, J A
AU  - Ledbetter JA
FAU - Tuel-Ahlgren, L
AU  - Tuel-Ahlgren L
FAU - Obuz, V
AU  - Obuz V
FAU - Waddick, K G
AU  - Waddick KG
FAU - Gajl-Peczalska, K
AU  - Gajl-Peczalska K
FAU - Schieven, G L
AU  - Schieven GL
FAU - Uckun, F M
AU  - Uckun FM
LA  - eng
GR  - R01 CA-42633/CA/NCI NIH HHS/United States
GR  - R01 CA-51425/CA/NCI NIH HHS/United States
GR  - R29 CA-42111/CA/NCI NIH HHS/United States
GR  - etc.
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - United States
TA  - Blood
JT  - Blood
JID - 7603509
RN  - 0 (Cytokines)
RN  - 0 (Interleukin-7)
RN  - 0 (Phosphatidylinositols)
RN  - 0 (Receptors, Immunologic)
RN  - 0 (Receptors, Interleukin-7)
RN  - 0 (Recombinant Proteins)
RN  - 85166-31-0 (Inositol 1,4,5-Trisphosphate)
RN  - EC 2.7.10.1 (Protein-Tyrosine Kinases)
SB  - IM
MH  - Bone Marrow/immunology/pathology
MH  - Cell Division
MH  - Clone Cells
MH  - Cytokines/*pharmacology
MH  - Enzyme Activation
MH  - Humans
MH  - Inositol 1,4,5-Trisphosphate/*metabolism
MH  - Interleukin-7/*pharmacology/physiology
MH  - Leukemia-Lymphoma, Adult T-Cell/*immunology/pathology
MH  - Lymphocyte Activation/drug effects
MH  - Phosphatidylinositols/*metabolism
MH  - Phosphorylation
MH  - Protein-Tyrosine Kinases/*metabolism
MH  - Receptors, Immunologic/*physiology
MH  - Receptors, Interleukin-7
MH  - Recombinant Proteins/pharmacology
MH  - T-Lymphocytes/drug effects/*immunology
EDAT- 1991/08/01 00:00
MHDA- 1991/08/01 00:01
CRDT- 1991/08/01 00:00
PHST- 1991/08/01 00:00 [pubmed]
PHST- 1991/08/01 00:01 [medline]
PHST- 1991/08/01 00:00 [entrez]
AID - S0006-4971(20)85220-3 [pii]
PST - ppublish
SO  - Blood. 1991 Aug 1;78(3):564-70.