PMID- 16504308
OWN - NLM
STAT- MEDLINE
DCOM- 20060706
LR  - 20161128
IS  - 0165-5728 (Print)
IS  - 0165-5728 (Linking)
VI  - 174
IP  - 1-2
DP  - 2006 May
TI  - Evidence that hypoxia-inducible factor-1 (HIF-1) mediates transcriptional 
      activation of interleukin-1beta (IL-1beta) in astrocyte cultures.
PG  - 63-73
AB  - Hypoxia-inducible factor-1 (HIF-1) is a heterodimeric transcription factor 
      composed of HIF-1alpha and HIF-1beta subunits and involved in the regulation of 
      gene expression in adaptive response to hypoxia. This study reports that the 
      inflammatory cytokine interleukin-1beta (IL-1beta) shares common features of 
      other known HIF-1alpha-regulated genes. Both human and mouse IL-1beta genes carry 
      multiple HIF-1-binding sites in their promoter regions and are up-regulated by 
      hypoxia and CoCl2 in human and mouse astrocytes in parallel with up-regulation of 
      HIF-1alpha mRNA and protein. Inhibition of HIF-1alpha degradation by proteasome 
      inhibitor, MG-132, potentiated hypoxia-induced IL-1beta release from human 
      astrocytes, and this response was blocked in the presence of CdCl2. Mouse 
      astrocytes with Hif1alpha+/- genotype demonstrated attenuated up-regulation of 
      both HIF-1alpha and IL-1beta by hypoxia and CoCl2. Mutation of HIF-1-binding 
      sites in the IL-1beta promoter abolished hypoxia-induced transactivation of the 
      reporter gene transfected into human astrocytes. Similarly, HIF-1 binding "decoy" 
      oligonuleotide transfected into astrocytes inhibited both hypoxia-induced 
      transactivation of the HIF-1 reporter gene and IL-1beta secretion from 
      transfected astrocytes. Collectively, the evidence suggests that the 
      transcriptional activation of IL-1beta in astrocytes exposed to hypoxia occurs 
      via HIF-1.
FAU - Zhang, Wandong
AU  - Zhang W
AD  - Cerebrovascular Research Group, Neurobiology Program, Institute for Biological 
      Sciences, National Research Council of Canada, Ottawa, Canada. 
      Wandong.Zhang@nrc-cnrc.gc.ca
FAU - Petrovic, Jelena-Mojsilovic
AU  - Petrovic JM
FAU - Callaghan, Debbie
AU  - Callaghan D
FAU - Jones, Aimee
AU  - Jones A
FAU - Cui, Hong
AU  - Cui H
FAU - Howlett, Clare
AU  - Howlett C
FAU - Stanimirovic, Danica
AU  - Stanimirovic D
LA  - eng
PT  - Comparative Study
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20060228
PL  - Netherlands
TA  - J Neuroimmunol
JT  - Journal of neuroimmunology
JID - 8109498
RN  - 0 (Antimutagenic Agents)
RN  - 0 (Cysteine Proteinase Inhibitors)
RN  - 0 (Hypoxia-Inducible Factor 1)
RN  - 0 (Interleukin-1)
RN  - 0 (Leupeptins)
RN  - 0 (RNA, Messenger)
RN  - 0 (Vascular Endothelial Growth Factor A)
RN  - 3G0H8C9362 (Cobalt)
RN  - EVS87XF13W (cobaltous chloride)
RN  - RF1P63GW3K (benzyloxycarbonylleucyl-leucyl-leucine aldehyde)
SB  - IM
MH  - Analysis of Variance
MH  - Animals
MH  - Antimutagenic Agents/pharmacology
MH  - Astrocytes/drug effects/*metabolism
MH  - Blotting, Western/methods
MH  - Cell Hypoxia/physiology
MH  - Cobalt/pharmacology
MH  - Cysteine Proteinase Inhibitors/pharmacology
MH  - Enzyme Activation/drug effects/physiology
MH  - Enzyme-Linked Immunosorbent Assay/methods
MH  - Fetus/cytology
MH  - Gene Expression Regulation/drug effects/*physiology
MH  - Humans
MH  - Hypoxia-Inducible Factor 1/*physiology
MH  - Interleukin-1/genetics/*metabolism
MH  - Leupeptins/pharmacology
MH  - Mice
MH  - Mice, Transgenic
MH  - Models, Biological
MH  - Mutagenesis/physiology
MH  - RNA, Messenger/metabolism
MH  - Reverse Transcriptase Polymerase Chain Reaction/methods
MH  - Time Factors
MH  - Transfection/methods
MH  - Vascular Endothelial Growth Factor A/metabolism
EDAT- 2006/03/01 09:00
MHDA- 2006/07/11 09:00
CRDT- 2006/03/01 09:00
PHST- 2005/09/02 00:00 [received]
PHST- 2006/01/19 00:00 [revised]
PHST- 2006/01/20 00:00 [accepted]
PHST- 2006/03/01 09:00 [pubmed]
PHST- 2006/07/11 09:00 [medline]
PHST- 2006/03/01 09:00 [entrez]
AID - S0165-5728(06)00016-6 [pii]
AID - 10.1016/j.jneuroim.2006.01.014 [doi]
PST - ppublish
SO  - J Neuroimmunol. 2006 May;174(1-2):63-73. doi: 10.1016/j.jneuroim.2006.01.014. 
      Epub 2006 Feb 28.