PMID- 16504689
OWN - NLM
STAT- MEDLINE
DCOM- 20060502
LR  - 20061115
IS  - 0041-1345 (Print)
IS  - 0041-1345 (Linking)
VI  - 38
IP  - 1
DP  - 2006 Jan-Feb
TI  - Immunocytological urinalysis and monocyte chemotactic peptide-1 in renal 
      transplant recipients with polyomavirus replication.
PG  - 151-4
AB  - In some patients polyomavirus replication induces chronic tubulointerstitial 
      inflammation in the transplanted kidney. The aim of this study was to investigate 
      whether immunocytological urinalysis and monocyte chemoattractant protein-1 
      (MCP-1) assays could be used for an early diagnosis of nephropathy for patients 
      with polyomavirus replication. We analyzed 1189 urine sediments from 174 renal 
      allograft recipients who were transplanted between 2000 and 2005. Decoy cells 
      were identified by an immunofluorescence method using specific antibodies (JC/BK 
      monoclonal antibody). A similar method was used to detect CD3(+), CD14(+), and 
      HLA-DR(+) cells with appropriate antibodies. The urinary excretion of MCP-1 was 
      assayed by enzyme-linked immunosorbent assay. The results of urine sediment 
      analysis and MCP-1 concentrations were compared with those of patients with 
      stable graft function (control group n = 65). In 17 patients (10%) decoy cells 
      were identified in urine. In 12 patients polyomavirus DNA was detected in plasma 
      or urine by a polymerase chain reaction method. Polyomavirus nephropathy was 
      diagnosed in eight patients by the presence of intranuclear viral inclusions or 
      immunohistochemical staining with SV40 large T-antigen specimens from a renal 
      biopsy, as well as by clinical and histopathological evidence (group I). 
      Polyomavirus replication was diagnosed in four patients by urinary excretion of 
      decoy cells and polyomavirus DNA detection (group III). In five patients only 
      decoy cells were found. The patients of groups I and II showed an increased 
      number of CD3, CD14, HLA-DR surface antigen-positive cells and greater excretion 
      of MCP-1 compared with the control group (P < .02). The number of excreted cells 
      was higher among patients with more severe infiltration. The results of patients 
      from group III were similar to the control group. In conclusion, increased 
      excretion of cells with CD3, CD14, and HLA-DR surface antigens and of MCP-1 were 
      associated with intragraft tubulointerstitial inflammation in patients with 
      polyomavirus nephropathy. Asymptomatic polyomavirus replication was associated 
      with hidden tubulointerstitial inflammation. Monitoring cell excretion and 
      chemokine content may be utilized for early detection of polyomavirus-induced 
      nephropathy.
FAU - Boratynska, M
AU  - Boratynska M
AD  - Department of Nephrology and Transplant Medicine, Wroclaw Medical University, ul. 
      Traugutta 57/59, 50-417 Wroclaw, Poland. borat@iitd.pan.wroc.pl
FAU - Dubinski, B
AU  - Dubinski B
FAU - Rybka, K
AU  - Rybka K
FAU - Jezior, D
AU  - Jezior D
FAU - Szyber, P
AU  - Szyber P
FAU - Klinger, M
AU  - Klinger M
LA  - eng
PT  - Journal Article
PL  - United States
TA  - Transplant Proc
JT  - Transplantation proceedings
JID - 0243532
RN  - 0 (Antigens, CD)
RN  - 0 (CCL2 protein, human)
RN  - 0 (Chemokine CCL2)
RN  - 0 (HLA-D Antigens)
RN  - 0 (HLA-DR Antigens)
SB  - IM
MH  - Adult
MH  - Antigens, CD/urine
MH  - Chemokine CCL2/*urine
MH  - Female
MH  - HLA-D Antigens/urine
MH  - HLA-DR Antigens/urine
MH  - Humans
MH  - Immunohistochemistry/methods
MH  - Kidney Transplantation/immunology/*physiology
MH  - Male
MH  - Middle Aged
MH  - Monitoring, Physiologic/methods
MH  - Polyomavirus/isolation & purification/*physiology
MH  - Retrospective Studies
MH  - Virus Replication
EDAT- 2006/03/01 09:00
MHDA- 2006/05/04 09:00
CRDT- 2006/03/01 09:00
PHST- 2006/03/01 09:00 [pubmed]
PHST- 2006/05/04 09:00 [medline]
PHST- 2006/03/01 09:00 [entrez]
AID - S0041-1345(05)01583-6 [pii]
AID - 10.1016/j.transproceed.2005.12.087 [doi]
PST - ppublish
SO  - Transplant Proc. 2006 Jan-Feb;38(1):151-4. doi: 
      10.1016/j.transproceed.2005.12.087.