PMID- 16506193
OWN - NLM
STAT- MEDLINE
DCOM- 20060609
LR  - 20181201
IS  - 0021-9967 (Print)
IS  - 0021-9967 (Linking)
VI  - 495
IP  - 6
DP  - 2006 Apr 20
TI  - Differential distributions of the Ca2+ -dependent activator protein for secretion 
      family proteins (CAPS2 and CAPS1) in the mouse brain.
PG  - 735-53
AB  - The Ca(2+)-dependent activator protein for secretion (CAPS/Cadps) family consists 
      of two members, CAPS1 and CAPS2, and plays an important role in secretory granule 
      exocytosis. It has been shown that CAPS1 regulates catecholamine release from 
      neuroendocrine cells, whereas CAPS2 is involved in the release of two 
      neurotrophins, brain-derived neurotrophic factor (BDNF) and neurotrophin-3 
      (NT-3), from parallel fibers of cerebellar granule cells. Although both CAPS 
      proteins are expressed predominantly in the brain, their cellular and regional 
      distributions in the brain are largely unknown. In this study we analyzed the 
      immunohistochemical distributions of the CAPS family proteins in the mouse brain. 
      In most areas of the embryonic nervous system CAPS1 and CAPS2 proteins were 
      complementarily expressed. In the postnatal brain, CAPS1 was widespread at 
      different levels. On the other hand, CAPS2 was localized to distinct cell types 
      and fibers of various brain regions, including the olfactory bulb, cerebrum, 
      hippocampal formation, thalamus, mesencephalic tegmentum, cerebellum, medulla, 
      and spinal cord, except for some regions that overlapped with CAPS1. These CAPS2 
      cellular distribution patterns had the marked feature of coinciding with those of 
      BDNF in various brain regions. Immunolabels for CAPS2 were also colocalized with 
      those for some proteins related to exocytosis (VAMP and SNAP-25) and endocytosis 
      (Dynamin I) in the cell soma and processes of the mesencephalic tegmentum and 
      cerebellum, suggesting that these proteins might be involved in the dynamics of 
      CAPS2-associated vesicles, although their colocalization on vesicles remains 
      elusive. These results demonstrate that the CAPS family proteins are involved in 
      the secretion of different secretory substances in developing and postnatal 
      brains, and that CAPS2 is probably involved in BDNF secretion in many brain 
      areas.
FAU - Sadakata, Tetsushi
AU  - Sadakata T
AD  - Laboratory for Molecular Neurogenesis, RIKEN Brain Science Institute, Wako, 
      Saitama 351-1098, Japan.
FAU - Itakura, Makoto
AU  - Itakura M
FAU - Kozaki, Shunji
AU  - Kozaki S
FAU - Sekine, Yukiko
AU  - Sekine Y
FAU - Takahashi, Masami
AU  - Takahashi M
FAU - Furuichi, Teiichi
AU  - Furuichi T
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - J Comp Neurol
JT  - The Journal of comparative neurology
JID - 0406041
RN  - 0 (Brain-Derived Neurotrophic Factor)
RN  - 0 (CAPS2 protein, mouse)
RN  - 0 (Cadps protein, mouse)
RN  - 0 (Calcium-Binding Proteins)
RN  - 0 (Nerve Tissue Proteins)
RN  - 0 (Protein Isoforms)
RN  - 0 (Synaptosomal-Associated Protein 25)
RN  - 0 (Vesicle-Associated Membrane Protein 1)
RN  - EC 3.5.1.50 (Dynamin I)
SB  - IM
MH  - Animals
MH  - Animals, Newborn
MH  - Brain/cytology/growth & development/*metabolism
MH  - Brain-Derived Neurotrophic Factor/*metabolism
MH  - Calcium-Binding Proteins/*metabolism
MH  - Cells, Cultured
MH  - Coculture Techniques
MH  - Dynamin I/metabolism
MH  - Endocytosis/physiology
MH  - Exocytosis/physiology
MH  - Immunohistochemistry
MH  - Mice
MH  - Mice, Inbred C57BL
MH  - Mice, Inbred ICR
MH  - Nerve Tissue Proteins/*metabolism
MH  - Neurons/cytology/*metabolism
MH  - Protein Isoforms/metabolism
MH  - Rats
MH  - Rats, Wistar
MH  - Synaptosomal-Associated Protein 25/metabolism
MH  - Transport Vesicles/metabolism
MH  - Vesicle-Associated Membrane Protein 1/metabolism
EDAT- 2006/03/01 09:00
MHDA- 2006/06/10 09:00
CRDT- 2006/03/01 09:00
PHST- 2006/03/01 09:00 [pubmed]
PHST- 2006/06/10 09:00 [medline]
PHST- 2006/03/01 09:00 [entrez]
AID - 10.1002/cne.20947 [doi]
PST - ppublish
SO  - J Comp Neurol. 2006 Apr 20;495(6):735-53. doi: 10.1002/cne.20947.