PMID- 16506892
OWN - NLM
STAT- MEDLINE
DCOM- 20060502
LR  - 20131121
IS  - 1540-658X (Print)
IS  - 1540-658X (Linking)
VI  - 4
IP  - 1
DP  - 2006 Feb
TI  - Application of cryopreserved cells to HERG screening using a non-radioactive Rb+ 
      efflux assay.
PG  - 83-8
AB  - The aim of the present study was to test the use of cryopreserved cells for human 
      ether-ago- go (hERG) channel screening. A stable Chinese hamster ovary-K1 cell 
      line expressing hERG channels was used in a non-radioactive Rb+ efflux assay for 
      screening hERG channel activity. Cells prepared from normal cultures and 
      following cryopreservation were compared with regard to time course of Rb+ efflux 
      response at different concentrations of KCl, overall cell morphology, signal-to 
      noise assay window, assay robustness, and 50% inhibitory concentration (IC50) of 
      the hERG blocker dofetilide. Our results showed that cryopreserved 
      hERG-expressing cells at 1, 2, and 3 days following plating at different cell 
      densities had no obvious morphological changes in comparison to the growing 
      cells. The assay window, Z' factor, and IC50 of dofetilide were also very similar 
      in all series of cells tested. It was concluded that the cryopreserved hERG cells 
      prepared using the protocol described are as effective as growing hERG cells in 
      the non-radioactive Rb+ efflux assay.
FAU - Ding, Mei
AU  - Ding M
AD  - Lead Discovery Sciences, Department of Molecular Pharmacology, AstraZeneca R&D 
      Molndal, Molndal, Sweden. mei.ding@astrazeneca.com
FAU - Stjernborg, Louise
AU  - Stjernborg L
FAU - Albertson, Nan
AU  - Albertson N
LA  - eng
PT  - Journal Article
PL  - United States
TA  - Assay Drug Dev Technol
JT  - Assay and drug development technologies
JID - 101151468
RN  - 0 (Ether-A-Go-Go Potassium Channels)
RN  - 0 (KCNH6 protein, human)
RN  - MLT4718TJW (Rubidium)
SB  - IM
MH  - Animals
MH  - Biological Transport
MH  - CHO Cells
MH  - Cell Culture Techniques/methods
MH  - Cricetinae
MH  - Cryopreservation/*methods
MH  - Ether-A-Go-Go Potassium Channels/*metabolism
MH  - Kinetics
MH  - Rubidium/*metabolism
EDAT- 2006/03/02 09:00
MHDA- 2006/05/04 09:00
CRDT- 2006/03/02 09:00
PHST- 2006/03/02 09:00 [pubmed]
PHST- 2006/05/04 09:00 [medline]
PHST- 2006/03/02 09:00 [entrez]
AID - 10.1089/adt.2006.4.83 [doi]
PST - ppublish
SO  - Assay Drug Dev Technol. 2006 Feb;4(1):83-8. doi: 10.1089/adt.2006.4.83.