PMID- 16507115
OWN - NLM
STAT- MEDLINE
DCOM- 20070129
LR  - 20181113
IS  - 1478-6362 (Electronic)
IS  - 1478-6354 (Print)
IS  - 1478-6354 (Linking)
VI  - 8
IP  - 1
DP  - 2006
TI  - Enumeration and phenotypical analysis of distinct dendritic cell subsets in 
      psoriatic arthritis and rheumatoid arthritis.
PG  - R15
AB  - Dendritic cells (DCs) comprise heterogeneous subsets of professional 
      antigen-presenting cells, linking innate and adaptive immunity. Analysis of DC 
      subsets has been hampered by a lack of specific DC markers and reliable 
      quantitation assays. We characterised the immunophenotype and functional 
      characteristics of psoriatic arthritis (PsA)-derived and rheumatoid arthritis 
      (RA)-derived myeloid DCs (mDCs) and plasmacytoid DCs (pDCs) to evaluate their 
      potential role in arthritis. Circulating peripheral blood (PB) pDC numbers were 
      significantly reduced in PsA patients (P = 0.0098) and RA patients (P = 0.0194), 
      and mDCs were significantly reduced in RA patients (P = 0.0086) compared with 
      healthy controls. The number of circulating mDCs in RA PB was significantly 
      inversely correlated to C-reactive protein (P = 0.021). The phenotype of both DC 
      subsets in PsA PB and RA PB was immature as compared with healthy controls. 
      Moreover, CD62L expression was significantly decreased on both mDCs (PsA, P = 
      0.0122; RA, P = 0.0371) and pDCs (PsA, P = 0.0373; RA, P = 0.0367) in PB. Both 
      mDCs and pDCs were present in PsA synovial fluid (SF) and RA SF, with the mDC:pDC 
      ratio significantly exceeding that in matched PB (PsA SF, P = 0.0453; RA SF, P = 
      0.0082). pDCs isolated from RA SF and PsA SF displayed an immature phenotype 
      comparable with PB pDCs. RA and PsA SF mDCs, however, displayed a more mature 
      phenotype (increased expression of CD80, CD83 and CD86) compared with PB mDCs. 
      Functional analysis revealed that both SF DC subsets matured following toll-like 
      receptor stimulation. pDCs from PB and SF produced interferon alpha and tumour 
      necrosis factor alpha on TLR9 stimulation, but only SF pDCs produced IL-10. 
      Similarly, mDCs from PB and SF produced similar tumour necrosis factor alpha 
      levels to TLR2 agonism, whereas SF mDCs produced more IL-10 than PB controls. 
      Circulating DC subset numbers are reduced in RA PB and PsA PB with reduced CD62L 
      expression. Maturation is incomplete in the inflamed synovial compartment. 
      Immature DCs in SF may contribute to the perpetuation of inflammation via 
      sampling of the inflamed synovial environment, and in situ presentation of 
      arthritogenic antigen.
FAU - Jongbloed, Sarah L
AU  - Jongbloed SL
AD  - Division of Immunology, Infection and Inflammation, 10 Alexandra Parade, Glasgow, 
      G31 2ER, UK. sj32w@clinmed.gla.ac.uk
FAU - Lebre, M Cristina
AU  - Lebre MC
FAU - Fraser, Alasdair R
AU  - Fraser AR
FAU - Gracie, J Alastair
AU  - Gracie JA
FAU - Sturrock, Roger D
AU  - Sturrock RD
FAU - Tak, Paul P
AU  - Tak PP
FAU - McInnes, Iain B
AU  - McInnes IB
LA  - eng
PT  - Comparative Study
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - England
TA  - Arthritis Res Ther
JT  - Arthritis research & therapy
JID - 101154438
RN  - 0 (Cytokines)
RN  - 9007-41-4 (C-Reactive Protein)
SB  - IM
MH  - Arthritis, Psoriatic/blood/*immunology/pathology
MH  - Arthritis, Rheumatoid/blood/*immunology/pathology
MH  - C-Reactive Protein/metabolism
MH  - Cytokines/*blood/genetics
MH  - Dendritic Cells/*immunology
MH  - Flow Cytometry
MH  - Humans
MH  - Immunophenotyping
MH  - Phenotype
MH  - Reference Values
MH  - Reverse Transcriptase Polymerase Chain Reaction
PMC - PMC1526567
EDAT- 2006/03/02 09:00
MHDA- 2007/01/30 09:00
PMCR- 2005/12/16
CRDT- 2006/03/02 09:00
PHST- 2005/06/13 00:00 [received]
PHST- 2005/10/20 00:00 [revised]
PHST- 2005/11/09 00:00 [accepted]
PHST- 2006/03/02 09:00 [pubmed]
PHST- 2007/01/30 09:00 [medline]
PHST- 2006/03/02 09:00 [entrez]
PHST- 2005/12/16 00:00 [pmc-release]
AID - ar1864 [pii]
AID - 10.1186/ar1864 [doi]
PST - ppublish
SO  - Arthritis Res Ther. 2006;8(1):R15. doi: 10.1186/ar1864.