PMID- 16507985
OWN - NLM
STAT- MEDLINE
DCOM- 20060620
LR  - 20220311
IS  - 0270-7306 (Print)
IS  - 1098-5549 (Electronic)
IS  - 0270-7306 (Linking)
VI  - 26
IP  - 6
DP  - 2006 Mar
TI  - Tankyrase 2 poly(ADP-ribose) polymerase domain-deleted mice exhibit growth 
      defects but have normal telomere length and capping.
PG  - 2044-54
AB  - Regulation of telomere length maintenance and capping are a critical cell 
      functions in both normal and tumor cells. Tankyrase 2 (Tnks2) is a 
      poly(ADP-ribose) polymerase (PARP) that has been shown to modify itself and TRF1, 
      a telomere-binding protein. We show here by overexpression studies that tankyrase 
      2, like its closely related homolog tankyrase 1, can function as a positive 
      regulator of telomere length in human cells, dependent on its catalytic PARP 
      activity. To study the role of Tnks2 in vivo, we generated mice with the Tnks2 
      PARP domain deleted. These mice are viable and fertile but display a growth 
      retardation phenotype. Telomere analysis by quantitative fluorescence in situ 
      hybridization (FISH), flow-FISH, and restriction fragment analysis showed no 
      change in telomere length or telomere capping in these mice. To determine the 
      requirement for Tnks2 in long-term maintenance of telomeres, we generated 
      embryonic stem cells with the Tnks2 PARP domain deleted and observed no change, 
      even upon prolonged growth, in telomere length or telomere capping. Together, 
      these results suggest that Tnks2 has a role in normal growth and development but 
      is not essential for telomere length maintenance or telomere capping in mice.
FAU - Hsiao, Susan J
AU  - Hsiao SJ
AD  - Skirball Institute of Biomolecular Medicine, New York University School of 
      Medicine, 540 First Avenue, 2nd Floor, New York, NY 10016, USA.
FAU - Poitras, Marc F
AU  - Poitras MF
FAU - Cook, Brandoch D
AU  - Cook BD
FAU - Liu, Yie
AU  - Liu Y
FAU - Smith, Susan
AU  - Smith S
LA  - eng
GR  - R01 CA95099/CA/NCI NIH HHS/United States
GR  - T32 GM007308/GM/NIGMS NIH HHS/United States
GR  - T32 GM007238/GM/NIGMS NIH HHS/United States
GR  - R01 CA095099/CA/NCI NIH HHS/United States
GR  - GM07308/GM/NIGMS NIH HHS/United States
GR  - GM07238/GM/NIGMS NIH HHS/United States
PT  - Journal Article
PT  - Research Support, N.I.H., Extramural
PT  - Research Support, Non-U.S. Gov't
PT  - Research Support, U.S. Gov't, Non-P.H.S.
PL  - United States
TA  - Mol Cell Biol
JT  - Molecular and cellular biology
JID - 8109087
RN  - EC 2.4.2.30 (Poly(ADP-ribose) Polymerases)
RN  - EC 2.4.2.30 (TNKS2 protein, human)
RN  - EC 2.4.2.30 (Tankyrases)
RN  - EC 2.4.4.30 (TNKS protein, human)
SB  - IM
MH  - Animals
MH  - Body Size/genetics
MH  - Cells, Cultured
MH  - Female
MH  - Growth/*genetics
MH  - Humans
MH  - Male
MH  - Mice
MH  - Mice, Mutant Strains/growth & development
MH  - Poly(ADP-ribose) Polymerases/metabolism
MH  - Protein Structure, Tertiary
MH  - Reference Values
MH  - Stem Cells/cytology/physiology
MH  - Tankyrases/*genetics/*metabolism
MH  - Telomere/*physiology
PMC - PMC1430302
EDAT- 2006/03/02 09:00
MHDA- 2006/06/21 09:00
PMCR- 2006/07/01
CRDT- 2006/03/02 09:00
PHST- 2006/03/02 09:00 [pubmed]
PHST- 2006/06/21 09:00 [medline]
PHST- 2006/03/02 09:00 [entrez]
PHST- 2006/07/01 00:00 [pmc-release]
AID - 26/6/2044 [pii]
AID - 2219-05 [pii]
AID - 10.1128/MCB.26.6.2044-2054.2006 [doi]
PST - ppublish
SO  - Mol Cell Biol. 2006 Mar;26(6):2044-54. doi: 10.1128/MCB.26.6.2044-2054.2006.