PMID- 16508260
OWN - NLM
STAT- MEDLINE
DCOM- 20060911
LR  - 20220310
IS  - 0006-3126 (Print)
IS  - 0006-3126 (Linking)
VI  - 90
IP  - 1
DP  - 2006
TI  - Effects of heat shock and hypoxia on neonatal neutrophil lipopolysaccharide 
      responses: altered apoptosis, Toll-like receptor-4 and CD11b expression compared 
      with adults.
PG  - 34-9
AB  - BACKGROUND: Dysfunctional inflammatory responses have been implicated in several 
      neonatal inflammatory disorders following infection and hypoxia. OBJECTIVES: We 
      aimed to study the effects of in vitro hypoxia and heat shock (HS) on normal 
      adult and newborn neutrophil migration (CD11b) and persistence (apoptosis) 
      following lipopolysaccharide (LPS) stimulation. METHODS: The mechanism for 
      altered LPS responses was assessed at the level of the LPS signalling receptors, 
      Toll-like receptor-4 (TLR-4), TLR-2 and CD14 expression in normal neonates and 
      adults. RESULTS: In adults, although hypoxia delayed neutrophil apoptosis, LPS 
      enhanced this response. In contrast, HS (42 degrees C) increased adult apoptotic 
      rates and abrogated the LPS responses. Both hypoxia and HS prevented the 
      LPS-induced increase in adult CD11b although it was unaltered in neonates. Adult 
      TLR-4 neutrophil expression was increased by LPS and hypoxia, and decreased in 
      HS, possibly explaining their variable LPS responsiveness. In contrast, neonatal 
      neutrophils were LPS hyporesponsive which may be mediated by failure of TLR-4 
      upregulation with LPS. CONCLUSIONS: Neonates do not have increased LPS 
      responsiveness in hypoxia or heat shock in vitro, which may prevent 
      hyperinflammation and thereby minimise tissue damage in inflammation or 
      infection.
CI  - Copyright 2006 S. Karger AG, Basel.
FAU - Molloy, Eleanor J
AU  - Molloy EJ
AD  - Department of Surgery, Conway Institute of Biomolecular and Biomedical Research, 
      Mater Misericordiae University Hospital, Dublin, Ireland. elesean@hotmail.com
FAU - O'Neill, Amanda J
AU  - O'Neill AJ
FAU - Doyle, Belinda T
AU  - Doyle BT
FAU - Grantham, Julie J
AU  - Grantham JJ
FAU - Taylor, Cormac T
AU  - Taylor CT
FAU - Sheridan-Pereira, Margaret
AU  - Sheridan-Pereira M
FAU - Fitzpatrick, John M
AU  - Fitzpatrick JM
FAU - Webb, David W
AU  - Webb DW
FAU - Watson, R William G
AU  - Watson RW
LA  - eng
GR  - Wellcome Trust/United Kingdom
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20060223
PL  - Switzerland
TA  - Biol Neonate
JT  - Biology of the neonate
JID - 0247551
RN  - 0 (CD11b Antigen)
RN  - 0 (Lipopolysaccharides)
RN  - 0 (TLR4 protein, human)
RN  - 0 (Toll-Like Receptor 4)
SB  - IM
MH  - Adult
MH  - Aging/physiology
MH  - Apoptosis/drug effects
MH  - CD11b Antigen/*genetics
MH  - Cell Hypoxia
MH  - Female
MH  - Fetal Blood/cytology
MH  - Gestational Age
MH  - Hot Temperature
MH  - Humans
MH  - Infant, Newborn
MH  - Lipopolysaccharides/*pharmacology
MH  - Male
MH  - Neutrophils/*cytology/drug effects/*physiology
MH  - Toll-Like Receptor 4/*genetics
EDAT- 2006/03/02 09:00
MHDA- 2006/09/12 09:00
CRDT- 2006/03/02 09:00
PHST- 2005/05/04 00:00 [received]
PHST- 2005/10/11 00:00 [accepted]
PHST- 2006/03/02 09:00 [pubmed]
PHST- 2006/09/12 09:00 [medline]
PHST- 2006/03/02 09:00 [entrez]
AID - 91743 [pii]
AID - 10.1159/000091743 [doi]
PST - ppublish
SO  - Biol Neonate. 2006;90(1):34-9. doi: 10.1159/000091743. Epub 2006 Feb 23.