PMID- 16509501
OWN - NLM
STAT- MEDLINE
DCOM- 20060317
LR  - 20231120
IS  - 0022-3085 (Print)
IS  - 0022-3085 (Linking)
VI  - 104
IP  - 2
DP  - 2006 Feb
TI  - Long-term recovery after bone marrow stromal cell treatment of traumatic brain 
      injury in rats.
PG  - 272-7
AB  - OBJECT: This study was designed to follow the effects of bone marrow stromal cell 
      (BMSC) administration in rats after traumatic brain injury (TBI) for a 3-month 
      period. METHODS: Forty adult female Wistar rats were injured by a controlled 
      cortical impact and, 1 week later, were injected intravenously with one of three 
      different doses of BMSCs (2 x 10(6), 4 x 10(6), or 8 x 10(6) cells per animal) 
      obtained in male rats. Control rats received phosphate-buffered saline (PBS). 
      Neurological function in these rats was studied using a neurological severity 
      scale (NSS). The rats were killed 3 months after injury, and immunohistochemical 
      stains were applied to brain samples to study the distribution of the BMSCs. 
      Additional brain samples were analyzed by quantitative enzyme-linked 
      immunosorbent assays to measure the expression of the growth factors 
      brain-derived neurotrophic factor (BDNF) and nerve growth factor (NGF). Three 
      months after injury, BMSCs were present in the injured brain and their number was 
      significantly greater in animals that received 4 x 10(6) or 8 x 10(6) BMSCs than 
      in animals that received 2 x 10(6) BMSCs. The cells were primarily distributed 
      around the lesion boundary zone. Functional outcome was significantly better in 
      rats that received 4 x 10(6) or 8 x 10(6) BMSCs, compared with control animals, 
      although no improvement was seen in animals that received 2 x 10(6) BMSCs. All 
      doses of BMSCs significantly increased the expression of BDNF but not that of 
      NGF; however, this increase was significantly larger in animals that received 4 x 
      10(6) or 8 x 10(6) BMSCs than in controls or animals that received 2 x 10(6) 
      BMSCs. CONCLUSIONS: In summary, when injected in rats after TBI, BMSCs are 
      present in the brain 3 months later and significantly improve functional outcome.
FAU - Mahmood, Asim
AU  - Mahmood A
AD  - Department of Neurosurgery, Henry Ford Health Sciences Center, Detroit, Michigan 
      48202, USA. nsaam@neuro.hfh.edu
FAU - Lu, Dunyue
AU  - Lu D
FAU - Qu, Changsheng
AU  - Qu C
FAU - Goussev, Anton
AU  - Goussev A
FAU - Chopp, Michael
AU  - Chopp M
LA  - eng
PT  - Journal Article
PL  - United States
TA  - J Neurosurg
JT  - Journal of neurosurgery
JID - 0253357
SB  - IM
MH  - Animals
MH  - *Bone Marrow Transplantation
MH  - Brain Injuries/pathology/*therapy
MH  - Cell Proliferation
MH  - Female
MH  - Immunohistochemistry
MH  - Rats
MH  - Rats, Wistar
MH  - Stromal Cells/*transplantation
MH  - Treatment Outcome
EDAT- 2006/03/03 09:00
MHDA- 2006/03/18 09:00
CRDT- 2006/03/03 09:00
PHST- 2006/03/03 09:00 [pubmed]
PHST- 2006/03/18 09:00 [medline]
PHST- 2006/03/03 09:00 [entrez]
AID - 10.3171/jns.2006.104.2.272 [doi]
PST - ppublish
SO  - J Neurosurg. 2006 Feb;104(2):272-7. doi: 10.3171/jns.2006.104.2.272.