PMID- 16510429
OWN - NLM
STAT- MEDLINE
DCOM- 20060810
LR  - 20071114
IS  - 1066-5099 (Print)
IS  - 1066-5099 (Linking)
VI  - 24
IP  - 2
DP  - 2006 Feb
TI  - Formation of pancreatic duct epithelium from bone marrow during neonatal 
      development.
PG  - 307-14
AB  - Recent reports suggest that bone marrow-derived cells engraft and differentiate 
      into pancreatic tissue at very low frequency after pancreatic injury. All such 
      studies have used adult recipients. The aim of our studies was to investigate the 
      potential of bone marrow to contribute to the exocrine and endocrine components 
      of the pancreas during the normal rapid growth of the organ that occurs during 
      the neonatal period. Five to ten million bone marrow cells from adult, male, 
      transgenic, green fluorescent protein (GFP) mice were injected into neonatal 
      nonobese diabetic/severely compromised immunodeficient/beta2microglobulin-null 
      mice 24 hours after birth. Two months after bone marrow transplantation, pancreas 
      tissue was analyzed with fluorescence immunohistochemistry and fluorescence in 
      situ hybridization (FISH). Co-staining of GFP, with anticytokeratin antibody, and 
      with FISH for the presence of donor Y chromosome indicated that up to 40% of 
      ducts (median 4.6%) contained epithelial cells derived from donor bone marrow. In 
      some of these donor-derived ducts, there were clusters of large and small ducts, 
      all comprised of GFP+ epithelium, suggesting that whole branching structures were 
      derived from donor bone marrow. In addition, rare cells that coexpressed GFP and 
      insulin were found within islets. Unlike pancreatic damage models, no bone 
      marrow-derived vascular endothelial cells were found. In contrast to the neonatal 
      recipients, bone marrow transplanted into adult mice rarely generated ductal 
      epithelium or islet cells (p<.05 difference between adult and neonate 
      transplants). These findings demonstrate the existence in bone marrow of 
      pluripotent stem cells or epithelial precursors that can migrate to the pancreas 
      and differentiate into complex organ-specific structures during the neonatal 
      period.
FAU - Wang, Xiuli
AU  - Wang X
AD  - Division of Research Immunology/BMT, Department of Pathology, Childrens Hospital 
      Los Angeles, 4650 Sunset Blvd., M.S. #62, Los Angeles, CA 90027, USA.
FAU - Ge, Shundi
AU  - Ge S
FAU - Gonzalez, Ignacio
AU  - Gonzalez I
FAU - McNamara, George
AU  - McNamara G
FAU - Rountree, C Barth
AU  - Rountree CB
FAU - Xi, Kenny Kezhe
AU  - Xi KK
FAU - Huang, Grace
AU  - Huang G
FAU - Bhushan, Anil
AU  - Bhushan A
FAU - Crooks, Gay M
AU  - Crooks GM
LA  - eng
GR  - R01-DK68719/DK/NIDDK NIH HHS/United States
PT  - Journal Article
PT  - Research Support, N.I.H., Extramural
PT  - Research Support, Non-U.S. Gov't
PL  - England
TA  - Stem Cells
JT  - Stem cells (Dayton, Ohio)
JID - 9304532
SB  - IM
MH  - Animals
MH  - Animals, Newborn/*physiology
MH  - Bone Marrow/*physiology
MH  - Bone Marrow Cells/physiology
MH  - *Bone Marrow Transplantation
MH  - Islets of Langerhans/physiology
MH  - Male
MH  - Mice
MH  - Mice, Inbred C57BL
MH  - Mice, Inbred NOD
MH  - Mice, Transgenic
MH  - Pancreas/*physiology
MH  - Pancreatic Ducts/cytology/*physiology
MH  - *Regeneration
EDAT- 2006/03/03 09:00
MHDA- 2006/08/11 09:00
CRDT- 2006/03/03 09:00
PHST- 2006/03/03 09:00 [pubmed]
PHST- 2006/08/11 09:00 [medline]
PHST- 2006/03/03 09:00 [entrez]
AID - 24/2/307 [pii]
AID - 10.1634/stemcells.2005-0052 [doi]
PST - ppublish
SO  - Stem Cells. 2006 Feb;24(2):307-14. doi: 10.1634/stemcells.2005-0052.