PMID- 16510843
OWN - NLM
STAT- MEDLINE
DCOM- 20060725
LR  - 20141120
IS  - 0379-864X (Print)
IS  - 0379-864X (Linking)
VI  - 31
IP  - 4
DP  - 2006 May
TI  - Modification of synapse formation of accessory olfactory bulb neurons by 
      coculture with vomeronasal neurons.
PG  - 371-8
AB  - Previously, a coculture system of accessory olfactory bulb (AOB) neurons and 
      vomeronasal (VN) neurons was established for studying the functional roles of AOB 
      neurons in pheromonal signal processing. In this study, the effect of VN neurons 
      on the development of AOB neurons was examined in a coculture system. Spine 
      density was quantitatively measured for various culture periods of 21, 28, 36, 
      and 42 days in vitro. The densities of dendritic spines were lower in the 
      coculture than in single culture for all periods in vitro. Synapse formation on 
      spines was analyzed immunocytochemically using an anti-synaptophysin antibody. 
      The ratio of the density of synaptophysin-immunopositive spine/total spine 
      density was larger in the coculture than in the single culture. The volume of 
      spine head was larger in the coculture than in single culture. These changes were 
      not observed in the coculture in which there was no physical contact between AOB 
      neurons and VN neurons. These observations suggest that synapse formation on the 
      spines of AOB neurons is modified by physical contact with VN neurons.
FAU - Ishimatsu, Yuuki
AU  - Ishimatsu Y
AD  - Laboratory of Anatomy and Cell Biology, Department of Neuroscience Basic 
      Technology, Tokyo Metropolitan Institute for Neuroscience, 2-6 Musashidai, Fuchu, 
      Tokyo 183-8526, Japan.
FAU - Moriya-Ito, Keiko
AU  - Moriya-Ito K
FAU - Muramoto, Kazuyo
AU  - Muramoto K
FAU - Ichikawa, Masumi
AU  - Ichikawa M
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20060301
PL  - England
TA  - Chem Senses
JT  - Chemical senses
JID - 8217190
RN  - 0 (Actins)
RN  - 0 (enhanced green fluorescent protein)
RN  - 147336-22-9 (Green Fluorescent Proteins)
SB  - IM
MH  - Actins/chemistry/metabolism
MH  - Animals
MH  - Cells, Cultured
MH  - Coculture Techniques
MH  - Dendritic Spines/physiology/ultrastructure
MH  - Green Fluorescent Proteins/chemistry/metabolism
MH  - Olfactory Bulb/*cytology/physiology
MH  - Olfactory Receptor Neurons/*cytology/metabolism/physiology
MH  - Rats
MH  - Rats, Wistar
MH  - Sensitivity and Specificity
MH  - Synapses/physiology/*ultrastructure
MH  - Vomeronasal Organ/*cytology/metabolism/physiology
EDAT- 2006/03/03 09:00
MHDA- 2006/07/26 09:00
CRDT- 2006/03/03 09:00
PHST- 2006/03/03 09:00 [pubmed]
PHST- 2006/07/26 09:00 [medline]
PHST- 2006/03/03 09:00 [entrez]
AID - bjj041 [pii]
AID - 10.1093/chemse/bjj041 [doi]
PST - ppublish
SO  - Chem Senses. 2006 May;31(4):371-8. doi: 10.1093/chemse/bjj041. Epub 2006 Mar 1.