PMID- 16512495
OWN - NLM
STAT- MEDLINE
DCOM- 20110404
LR  - 20181201
VI  - 19
IP  - 24
DP  - 2005 Dec
TI  - [Induction of apoptosis and inhibition of proliferation in Hep-2 by survivin gene 
      targeting in vitro].
PG  - 1138-41
AB  - OBJECTIVE: To study induction of apoptosis and inhibition of proliferation in 
      Hep-2 by survivin gene targeting. METHOD: Antisense survivin RNA expression 
      vector was constructed and then was transfected to human laryngeal carcinoma cell 
      line Hep-2 by lipofectamine. HpEGFP/survivin cells were abstained by using G418. 
      The level of survivin protein before and after transfection was determined by 
      Western blot. Proliferation activity was measured by MTT assay. The experiment of 
      colony formation in soft agar was carried out. Apoptosis was assessed by flow 
      cytometry and acridine orange (AO). RESULT: After antisense survivin RNA plasmids 
      were transfected, the level of survivin protein was inhibited in Hep-2. Compared 
      with control, proliferation of HpEGFP/survivin cells transfected with the 
      recombinant of antisense survivin RNA were suppressed significantly. The 
      experiment of colony formation in soft agar showed the ability of colony 
      formation decreased in HpEGFP/survivin cells compared to control (P <0.05). 
      Apoptosis rate increased about 1.81 folds compared with control. CONCLUSION: The 
      recombination antisense survivin RNA plasmid can partly inhibit the level of 
      survivin protein expression in Hep-2. Antisense survivin RNA can induce apoptosis 
      and inhibit the proliferation of Hep-2 by down-regulating the expression of 
      endogenous survivin in vitro.
FAU - Wen, Lianji
AU  - Wen L
AD  - Department of Otorhinolaryngology, the Second Hospital of Jilin University, 
      Changchun, China.
FAU - Li, Changqing
AU  - Li C
FAU - Li, Ye
AU  - Li Y
FAU - Zhao, Dan
AU  - Zhao D
FAU - Gao, Lifang
AU  - Gao L
FAU - Wang, Lihua
AU  - Wang L
FAU - Guan, Guofang
AU  - Guan G
FAU - Jin, Chunshun
AU  - Jin C
LA  - chi
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - China
TA  - Lin Chuang Er Bi Yan Hou Ke Za Zhi
JT  - Lin chuang er bi yan hou ke za zhi = Journal of clinical otorhinolaryngology
JID - 9426080
RN  - 0 (BIRC5 protein, human)
RN  - 0 (Inhibitor of Apoptosis Proteins)
RN  - 0 (Microtubule-Associated Proteins)
RN  - 0 (RNA, Antisense)
RN  - 0 (Survivin)
SB  - IM
MH  - *Apoptosis
MH  - Cell Line, Tumor
MH  - *Cell Proliferation
MH  - *Gene Targeting
MH  - Humans
MH  - Inhibitor of Apoptosis Proteins
MH  - Laryngeal Neoplasms/pathology
MH  - Microtubule-Associated Proteins/*genetics
MH  - Plasmids
MH  - RNA, Antisense/genetics
MH  - Survivin
MH  - Transfection
EDAT- 2006/03/04 09:00
MHDA- 2011/04/05 06:00
CRDT- 2006/03/04 09:00
PHST- 2006/03/04 09:00 [pubmed]
PHST- 2011/04/05 06:00 [medline]
PHST- 2006/03/04 09:00 [entrez]
PST - ppublish
SO  - Lin Chuang Er Bi Yan Hou Ke Za Zhi. 2005 Dec;19(24):1138-41.