PMID- 1651253
OWN - NLM
STAT- MEDLINE
DCOM- 19910917
LR  - 20131121
IS  - 0301-472X (Print)
IS  - 0301-472X (Linking)
VI  - 19
IP  - 8
DP  - 1991 Sep
TI  - Dibutyryl cyclic AMP induces formyl peptide receptor expression and chemotactic 
      responses in a human eosinophilic cell line, EoL-1.
PG  - 823-8
AB  - We investigated actin polymerization and the increase of cytosolic free calcium 
      concentration ([Ca2+]i) in a human eosinophilic leukemia cell line, EoL-1, in 
      response to stimulation with chemotactic factors; we also investigated the effect 
      of dibutyryl cyclic AMP (dbcAMP) on the responses. EoL-1 cells under normal 
      culture conditions did not show either actin polymerization or an increase in 
      [Ca2+]i when stimulated with formyl-methionyl-leucyl-phenylalanine (fMLP). 
      Expression of formyl peptide receptors was not detectable on untreated EoL-1 
      cells, either. Dibutyryl cAMP induced the expression of formyl peptide receptors 
      and the responsiveness to fMLP. The responsiveness of EoL-1 cells to the 
      complement fragment C5a and platelet-activating factor (PAF) was also induced or 
      enhanced by dbcAMP. The growth of EoL-1 cells was decreased and the proportion of 
      cells in the G0/G1 phase of the cell cycle was increased by the treatment of 
      EoL-1 cells with dbcAMP. The proportion of eosinophilic granule-containing cells 
      and the content of eosinophil cationic protein (ECP) in EoL-1 cells was also 
      increased when they were stimulated with dbcAMP for a longer period. The 
      responsiveness of EoL-1 cells to fMLP, C5a, and PAF was shown to be regulated 
      independently. EoL-1 cells and dbcAMP seem to be useful for examining chemotactic 
      receptor expression and its signal transduction mechanisms.
FAU - Uenoyama, Y
AU  - Uenoyama Y
AD  - Department of Pediatrics, Faculty of Medicine, Kyoto University, Japan.
FAU - Ohshima, Y
AU  - Ohshima Y
FAU - Morita, M
AU  - Morita M
FAU - Akutagawa, H
AU  - Akutagawa H
FAU - Nambu, M
AU  - Nambu M
FAU - Kim, K M
AU  - Kim KM
FAU - Mayumi, M
AU  - Mayumi M
FAU - Mikawa, H
AU  - Mikawa H
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - Netherlands
TA  - Exp Hematol
JT  - Experimental hematology
JID - 0402313
RN  - 0 (Actins)
RN  - 0 (Butyrates)
RN  - 0 (Platelet Activating Factor)
RN  - 0 (Polymers)
RN  - 0 (Receptors, Formyl Peptide)
RN  - 0 (Receptors, Immunologic)
RN  - 107-92-6 (Butyric Acid)
RN  - 59880-97-6 (N-Formylmethionine Leucyl-Phenylalanine)
RN  - 63X7MBT2LQ (Bucladesine)
RN  - 80295-54-1 (Complement C5a)
RN  - SY7Q814VUP (Calcium)
SB  - IM
MH  - Actins/metabolism
MH  - Bucladesine/*pharmacology
MH  - Butyrates/pharmacology
MH  - Butyric Acid
MH  - Calcium/physiology
MH  - Cell Differentiation/drug effects
MH  - Cell Division/drug effects
MH  - Cell Line
MH  - Chemotaxis, Leukocyte/*drug effects
MH  - Complement C5a/pharmacology
MH  - Eosinophils/cytology/drug effects/*physiology
MH  - Humans
MH  - N-Formylmethionine Leucyl-Phenylalanine/metabolism
MH  - Platelet Activating Factor/pharmacology
MH  - Polymers
MH  - Receptors, Formyl Peptide
MH  - Receptors, Immunologic/*metabolism
MH  - Time Factors
EDAT- 1991/09/01 00:00
MHDA- 1991/09/01 00:01
CRDT- 1991/09/01 00:00
PHST- 1991/09/01 00:00 [pubmed]
PHST- 1991/09/01 00:01 [medline]
PHST- 1991/09/01 00:00 [entrez]
PST - ppublish
SO  - Exp Hematol. 1991 Sep;19(8):823-8.