PMID- 16512610
OWN - NLM
STAT- MEDLINE
DCOM- 20060317
LR  - 20061115
IS  - 0208-0613 (Print)
IS  - 0208-0613 (Linking)
IP  - 1
DP  - 2006
TI  - [Her-2 gene dosage analysis in human breast cancer by PCR with internal 
      standard].
PG  - 34-8
AB  - PCR assay with internal control was developed to quantify the her-2 gene dosage 
      in human breast cancer tumor samples. Recombinant plasmid with a fragment of the 
      her-2 gene containing the fragment of T7 phage DNA was designed especially to be 
      used as an internal control in the PCR her-2 gene dosage assay. PCR conditions 
      were optimized for the simultaneous usage of two templates--human genomic DNA and 
      DNA of recombinant plasmid (internal control). The ability of the technology to 
      discriminate a twofold increase of the her-2 gene dosage was demonstrated. 
      Increased level of her-2 gene amplification was observed in 6 of 38 samples 
      investigated. This new simple rapid assay can be an alternative to fluorescence 
      in situ hybridization (FISH) and immunochemistry (ICH) tests for the detection of 
      her-2 amplification in human tumors. This technique may be a useful tool for 
      large randomized, prospective cooperative group trials and may support selection 
      of optimal therapy for breast cancer patients in the future.
FAU - Matsenko, N Iu
AU  - Matsenko NIu
FAU - Kovalenko, S P
AU  - Kovalenko SP
LA  - rus
PT  - English Abstract
PT  - Journal Article
PL  - Russia (Federation)
TA  - Mol Gen Mikrobiol Virusol
JT  - Molekuliarnaia genetika, mikrobiologiia i virusologiia
JID - 9315607
RN  - 0 (DNA, Viral)
SB  - IM
MH  - Bacteriophage T7/genetics
MH  - Breast Neoplasms/diagnosis/*genetics
MH  - Carcinoma/diagnosis/*genetics
MH  - DNA, Viral
MH  - *Gene Dosage
MH  - Genes, erbB-2/*genetics
MH  - Humans
MH  - Plasmids
MH  - Polymerase Chain Reaction/*methods
MH  - Sensitivity and Specificity
MH  - Templates, Genetic
EDAT- 2006/03/04 09:00
MHDA- 2006/03/18 09:00
CRDT- 2006/03/04 09:00
PHST- 2006/03/04 09:00 [pubmed]
PHST- 2006/03/18 09:00 [medline]
PHST- 2006/03/04 09:00 [entrez]
PST - ppublish
SO  - Mol Gen Mikrobiol Virusol. 2006;(1):34-8.