PMID- 16512812
OWN - NLM
STAT- MEDLINE
DCOM- 20060823
LR  - 20220317
IS  - 0105-4538 (Print)
IS  - 0105-4538 (Linking)
VI  - 61
IP  - 4
DP  - 2006 Apr
TI  - Allergenicity assessment of transgenic mustard (Brassica juncea) expressing 
      bacterial codA gene.
PG  - 491-7
AB  - BACKGROUND: Assessing the allergenicity and toxicity of genetically modified (GM) 
      crops is essential before they become a regular part of our food supply. The 
      present study aimed to assess the allergenicity of Brassica juncea (mustard) 
      expressing choline oxidase (codA) gene from Arthrobacter globiformis that 
      provides resistance against abiotic stresses. METHODS: SDAP, Farrp, and 
      Swiss-Prot databases were used to study allergenicity of choline oxidase. 
      Digestibility of choline oxidase was assessed in simulated gastric fluid (SGF). 
      Specific immunoglobulin E (IgE) reactivity of native and GM mustard was compared 
      by using enzyme-linked immunosorbent assay (ELISA) and skin tests in 
      respiratory-allergic patients. Allergenicity of GM and native mustard proteins 
      was compared in Balb/c mice. RESULTS: Choline oxidase showed no significant 
      homology with allergenic proteins in SDAP and Farrp databases. Cross-reactive 
      epitope search showed a stretch similar to Hev b 6 having some antigenic 
      properties. Purified choline oxidase showed complete degradation with SGF. Skin 
      prick test of native and GM mustard extract on respiratory allergic patients 
      showed significant correlation (P < 0.05). ELISA with 96 patients' sera showed 
      comparable IgE reactivity. Balb/c mice immunized with native and GM mustard 
      proteins showed low IgE response. Presensitized mice on intravenous challenge 
      with Brassica extract showed no anaphylactic symptoms unlike ovalbumin (OVA) 
      sensitization that showed anaphylactic reaction in mice. Lung histology of 
      OVA-sensitized mice showed narrowing of airway and large eosinophilic 
      infiltration, whereas native and GM Brassica extract showed normal airway. 
      CONCLUSION: Genetically modified mustard with the codA gene possessed 
      allergenicity similar to that of native mustard and no enhancement of IgE binding 
      was observed due to genetic manipulation.
FAU - Singh, A K
AU  - Singh AK
AD  - Institute of Genomics and Integrative Biology, Delhi, India.
FAU - Mehta, A K
AU  - Mehta AK
FAU - Sridhara, S
AU  - Sridhara S
FAU - Gaur, S N
AU  - Gaur SN
FAU - Singh, B P
AU  - Singh BP
FAU - Sarma, P U
AU  - Sarma PU
FAU - Arora, N
AU  - Arora N
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - Denmark
TA  - Allergy
JT  - Allergy
JID - 7804028
RN  - 37341-29-0 (Immunoglobulin E)
RN  - 9006-59-1 (Ovalbumin)
RN  - EC 1.1.- (Alcohol Oxidoreductases)
RN  - EC 1.1.3.17 (choline oxidase)
SB  - IM
MH  - Adolescent
MH  - Adult
MH  - Alcohol Oxidoreductases/chemistry/*genetics
MH  - Animals
MH  - Arthrobacter/*enzymology
MH  - Female
MH  - Food Hypersensitivity/*etiology
MH  - *Food, Genetically Modified
MH  - Humans
MH  - Immunoglobulin E/blood
MH  - Lung/pathology
MH  - Male
MH  - Mice
MH  - Mice, Inbred BALB C
MH  - Mustard Plant/*genetics
MH  - Ovalbumin/immunology
MH  - Plants, Genetically Modified
MH  - Sequence Homology, Amino Acid
EDAT- 2006/03/04 09:00
MHDA- 2006/08/24 09:00
CRDT- 2006/03/04 09:00
PHST- 2006/03/04 09:00 [pubmed]
PHST- 2006/08/24 09:00 [medline]
PHST- 2006/03/04 09:00 [entrez]
AID - ALL1049 [pii]
AID - 10.1111/j.1398-9995.2006.01049.x [doi]
PST - ppublish
SO  - Allergy. 2006 Apr;61(4):491-7. doi: 10.1111/j.1398-9995.2006.01049.x.