PMID- 16513671
OWN - NLM
STAT- MEDLINE
DCOM- 20060712
LR  - 20181113
IS  - 0022-3751 (Print)
IS  - 1469-7793 (Electronic)
IS  - 0022-3751 (Linking)
VI  - 573
IP  - Pt 1
DP  - 2006 May 15
TI  - Interstitial cells of Cajal in the deep muscular plexus mediate enteric motor 
      neurotransmission in the mouse small intestine.
PG  - 147-59
AB  - Interstitial cells of Cajal (ICC) provide important regulatory functions in the 
      motor activity of the gastrointestinal tract. In the small intestine, ICC in the 
      myenteric region (ICC-MY), between the circular and longitudinal muscle layers, 
      generate and propagate electrical slow waves. Another population of ICC lies in 
      the plane of the deep muscular plexus (ICC-DMP), and these cells are closely 
      associated with varicose nerve terminals of enteric motor neurons. Here we tested 
      the hypothesis that ICC-DMP mediate excitatory and inhibitory neural inputs in 
      the small bowel. ICC-DMP develop largely after birth. ICC-DMP, with receptor 
      tyrosine kinase Kit-like immunoreactivity, appear first in the jejunum and then 
      in the ileum. We performed electrophysiological experiments on mice immediately 
      after birth (P0) or at 10 days post partum (P10) to determine whether neural 
      responses follow development of ICC-DMP. At P0, slow-wave activity was present in 
      the jejunum, but neural responses were poorly developed. By P10, after ICC-DMP 
      developed, both cholinergic excitatory and nitrergic inhibitory neural responses 
      were intact. Muscles of P0 mice were also put into organotypic cultures and 
      treated with a neutralizing Kit antibody. Neural responses developed in culture 
      within 3-6 days in control muscles, but blocking Kit caused loss of ICC and loss 
      of cholinergic and nitrergic neural responses. Non-cholinergic excitatory 
      responses remained after loss of ICC-DMP. Our observations are consistent with 
      the idea that cholinergic and nitrergic motor neural inputs are mediated, to a 
      large extent, via ICC-DMP. Thus, ICC-DMP appear to serve a function in the small 
      intestine that is similar to the role of the intramuscular ICC in the stomach.
FAU - Ward, Sean M
AU  - Ward SM
AD  - Department of Physiology and Cell Biology, University of Nevada School of 
      Medicine, Reno, NV 89557, USA.
FAU - McLaren, Gerald J
AU  - McLaren GJ
FAU - Sanders, Kenton M
AU  - Sanders KM
LA  - eng
GR  - P01 DK041315/DK/NIDDK NIH HHS/United States
GR  - DK41315/DK/NIDDK NIH HHS/United States
PT  - Journal Article
PT  - Research Support, N.I.H., Extramural
DEP - 20060302
PL  - England
TA  - J Physiol
JT  - The Journal of physiology
JID - 0266262
SB  - IM
MH  - Animals
MH  - Electrophysiology
MH  - Jejunum/cytology/*innervation/*physiology
MH  - Mice
MH  - Mice, Inbred BALB C
MH  - Motor Neurons/*physiology
MH  - Muscle, Smooth/cytology/innervation/physiology
MH  - Myenteric Plexus/*cytology/*physiology
MH  - Neural Inhibition/physiology
MH  - Neuromuscular Junction/physiology
MH  - Organ Culture Techniques
MH  - Synaptic Transmission/physiology
PMC - PMC1779710
EDAT- 2006/03/04 09:00
MHDA- 2006/07/13 09:00
PMCR- 2007/05/15
CRDT- 2006/03/04 09:00
PHST- 2006/03/04 09:00 [pubmed]
PHST- 2006/07/13 09:00 [medline]
PHST- 2006/03/04 09:00 [entrez]
PHST- 2007/05/15 00:00 [pmc-release]
AID - jphysiol.2006.105189 [pii]
AID - 10.1113/jphysiol.2006.105189 [doi]
PST - ppublish
SO  - J Physiol. 2006 May 15;573(Pt 1):147-59. doi: 10.1113/jphysiol.2006.105189. Epub 
      2006 Mar 2.