PMID- 16513879
OWN - NLM
STAT- MEDLINE
DCOM- 20060425
LR  - 20131121
IS  - 0002-953X (Print)
IS  - 0002-953X (Linking)
VI  - 163
IP  - 3
DP  - 2006 Mar
TI  - BDNF Val66Met polymorphism and GAD67 mRNA expression in the prefrontal cortex of 
      subjects with schizophrenia.
PG  - 534-7
AB  - OBJECTIVE: In the prefrontal cortex of subjects with schizophrenia, decreased 
      signaling mediated by brain-derived neurotrophic factor (BDNF) and its receptor 
      tyrosine kinase (TrkB) appears to contribute to the reduced expression of mRNA 
      encoding the 67-kilodalton isoform of glutamate decarboxylase (GAD(67)), an 
      enzyme for GABA synthesis. The authors examined in subjects with schizophrenia 
      the effect in the human BDNF gene of a single nucleotide polymorphism (Val66Met), 
      which reduces the trafficking and secretion of BDNF protein, on the expression of 
      GAD(67) mRNA. METHOD: BDNF Val66Met genotyping was performed in 27 matched pairs 
      of schizophrenia and comparison subjects. The impact of this polymorphism on 
      prefrontal cortex GAD(67) mRNA expression in schizophrenia subjects was assessed 
      by comparing within-pair differences in GAD(67) mRNA expression between 
      schizophrenia subjects with versus without the Met66 allele after the level of 
      BDNF mRNA expression was controlled. RESULTS: In contrast to expectations, the 
      within-pair reduction in GAD(67) mRNA expression was not greater in schizophrenia 
      subjects who were hetero- or homozygous for the Met66 allele. These subjects did 
      tend to exhibit less marked within-pair reductions in both GAD(67) and BDNF mRNA 
      expression compared with schizophrenia subjects homozygous for the Val allele. 
      CONCLUSIONS: The presence of the BDNF Met66 allele does not contribute to the 
      decreased level of GAD(67) mRNA expression in the prefrontal cortex of subjects 
      with schizophrenia.
FAU - Hashimoto, Takanori
AU  - Hashimoto T
AD  - Department of Psychiatry, University of Pittsburgh, PA 15213, USA.
FAU - Lewis, David A
AU  - Lewis DA
LA  - eng
GR  - MH-43784/MH/NIMH NIH HHS/United States
GR  - MH-45156/MH/NIMH NIH HHS/United States
PT  - Comparative Study
PT  - Journal Article
PT  - Research Support, N.I.H., Extramural
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Am J Psychiatry
JT  - The American journal of psychiatry
JID - 0370512
RN  - 0 (Brain-Derived Neurotrophic Factor)
RN  - 0 (Isoenzymes)
RN  - 0 (RNA, Messenger)
RN  - 56-12-2 (gamma-Aminobutyric Acid)
RN  - AE28F7PNPL (Methionine)
RN  - EC 2.7.10.1 (Receptor, trkB)
RN  - EC 4.1.1.15 (Glutamate Decarboxylase)
RN  - EC 4.1.1.15 (glutamate decarboxylase 1)
RN  - Q3JTX2Q7TU (Diazepam)
SB  - IM
MH  - Brain-Derived Neurotrophic Factor/*genetics/metabolism
MH  - Cohort Studies
MH  - Diazepam/metabolism
MH  - Female
MH  - Gene Expression
MH  - Gene Frequency
MH  - Genotype
MH  - Glutamate Decarboxylase/*genetics/*metabolism
MH  - Humans
MH  - Isoenzymes/*genetics/*metabolism
MH  - Male
MH  - Methionine
MH  - *Polymorphism, Single Nucleotide
MH  - Prefrontal Cortex/*metabolism
MH  - RNA, Messenger/genetics/metabolism
MH  - Receptor, trkB/genetics/metabolism
MH  - Schizophrenia/*genetics/metabolism
MH  - gamma-Aminobutyric Acid/biosynthesis
EDAT- 2006/03/04 09:00
MHDA- 2006/04/28 09:00
CRDT- 2006/03/04 09:00
PHST- 2006/03/04 09:00 [pubmed]
PHST- 2006/04/28 09:00 [medline]
PHST- 2006/03/04 09:00 [entrez]
AID - 163/3/534 [pii]
AID - 10.1176/appi.ajp.163.3.534 [doi]
PST - ppublish
SO  - Am J Psychiatry. 2006 Mar;163(3):534-7. doi: 10.1176/appi.ajp.163.3.534.