PMID- 16513925
OWN - NLM
STAT- MEDLINE
DCOM- 20060623
LR  - 20081121
IS  - 0022-0949 (Print)
IS  - 0022-0949 (Linking)
VI  - 209
IP  - Pt 6
DP  - 2006 Mar
TI  - Temperature regulates hypoxia-inducible factor-1 (HIF-1) in a poikilothermic 
      vertebrate, crucian carp (Carassius carassius).
PG  - 994-1003
AB  - Hypoxia-inducible transcription factor-1 (HIF-1) is a master regulator of 
      hypoxia-induced gene responses. To find out whether HIF-1 function is involved in 
      gene expression changes associated with temperature acclimation as well as in 
      hypoxia adaptation in poikilotherms, we studied HIF-1 DNA binding activity and 
      HIF-1alpha expression in normoxia and during hypoxia (0.7 mg l(-1) O2) in crucian 
      carp at temperatures of 26, 18 and 8 degrees C. Temperature had a marked 
      influence on HIF-1 in normoxia. Although HIF-1alpha mRNA levels remained 
      unaltered, cold acclimation (8 degrees C) increased HIF-1alpha protein amounts in 
      the liver, gills and heart and HIF-1 DNA binding activity in the heart, gills and 
      kidney of crucian carp by two- to threefold compared to warm acclimated fish (26 
      degrees C). In the heart and kidney HIF-1 activity was already significantly 
      increased in the 18 degrees C acclimated fish. Temperature also affected hypoxic 
      regulation of HIF-1. Although hypoxia initially increased amounts of HIF-1alpha 
      protein in all studied tissues at every temperature, except for liver at 18 
      degrees C, HIF-1 activity increased only in the heart of 8 degrees C acclimated 
      and in the gills of 18 degrees C acclimated fish. At 8 degrees C HIF-1alpha mRNA 
      levels increased transiently in the gills after 6 h of hypoxia and in the kidney 
      after 48 h of hypoxia. In the gills at 26 degrees C HIF-1alpha mRNA levels 
      increased after 6 h of hypoxia and remained above normoxic levels for up to 48 h 
      of hypoxia. These results show that HIF-1 is involved in controlling gene 
      responses to both oxygen and temperature in crucian carp. No overall 
      transcriptional control mechanism has been described for low temperature 
      acclimation in poikilotherms, but the present results suggest that HIF-1 could 
      have a role in such regulation. Moreover, this study highlights interaction of 
      the two prime factors defining metabolism, temperature and oxygen, in the 
      transcriptional control of metabolic homeostasis in animals.
FAU - Rissanen, Eeva
AU  - Rissanen E
AD  - Centre of Excellence in Evolutionary Genetics and Physiology, Department of 
      Biology, University of Turku, Finland. eva.rissanen@utu.fi
FAU - Tranberg, Hanna K
AU  - Tranberg HK
FAU - Sollid, Jorund
AU  - Sollid J
FAU - Nilsson, Goran E
AU  - Nilsson GE
FAU - Nikinmaa, Mikko
AU  - Nikinmaa M
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - England
TA  - J Exp Biol
JT  - The Journal of experimental biology
JID - 0243705
RN  - 0 (Hypoxia-Inducible Factor 1)
RN  - 0 (RNA, Messenger)
SB  - IM
MH  - Acclimatization
MH  - Animals
MH  - Body Temperature Regulation
MH  - Carps/*physiology
MH  - Cold Temperature
MH  - *Gene Expression Regulation
MH  - Gills/physiology
MH  - Heart/physiology
MH  - Hypoxia-Inducible Factor 1/*genetics
MH  - Kidney/physiology
MH  - Liver/physiology
MH  - RNA, Messenger/genetics
EDAT- 2006/03/04 09:00
MHDA- 2006/06/24 09:00
CRDT- 2006/03/04 09:00
PHST- 2006/03/04 09:00 [pubmed]
PHST- 2006/06/24 09:00 [medline]
PHST- 2006/03/04 09:00 [entrez]
AID - 209/6/994 [pii]
AID - 10.1242/jeb.02103 [doi]
PST - ppublish
SO  - J Exp Biol. 2006 Mar;209(Pt 6):994-1003. doi: 10.1242/jeb.02103.