PMID- 1651403
OWN - NLM
STAT- MEDLINE
DCOM- 19910919
LR  - 20200724
IS  - 0022-538X (Print)
IS  - 1098-5514 (Electronic)
IS  - 0022-538X (Linking)
VI  - 65
IP  - 9
DP  - 1991 Sep
TI  - Characterization of the DNA polymerase gene of human herpesvirus 6.
PG  - 4670-80
AB  - The construction of a recombinant bacteriophage lambda library containing 
      overlapping clones covering 155 kbp of the 161-kbp genome of the Ugandan U1102 
      isolate of human herpesvirus 6 (HHV-6) is described. The use of degenerate-primer 
      polymerase chain reaction allowed the isolation of a DNA probe for the DNA 
      polymerase gene of HHV-6, which was subsequently used to isolate and position the 
      pol gene on the physical map of the viral genome. A 4.4-kbp EcoRI DNA restriction 
      fragment containing the pol gene was isolated and sequenced. The open reading 
      frames flanking the pol gene code for the HHV-6 glycoprotein B gene and the human 
      cytomegalovirus UL53 homolog. This arrangement is different from that seen in the 
      alpha and gamma herpesvirus families, lending further support to the notion that 
      HHV-6 is a member of the beta herpesvirus group.
FAU - Teo, I A
AU  - Teo IA
AD  - Department of Virology, Royal Postgraduate Medical School, London, England.
FAU - Griffin, B E
AU  - Griffin BE
FAU - Jones, M D
AU  - Jones MD
LA  - eng
SI  - GENBANK/M63042
SI  - GENBANK/M63043
SI  - GENBANK/M63044
SI  - GENBANK/M63045
SI  - GENBANK/M63046
SI  - GENBANK/M63047
SI  - GENBANK/M63048
SI  - GENBANK/M63804
SI  - GENBANK/M87045
SI  - GENBANK/S49268
GR  - Wellcome Trust/United Kingdom
PT  - Comparative Study
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - J Virol
JT  - Journal of virology
JID - 0113724
RN  - 0 (Codon)
RN  - 0 (DNA, Viral)
RN  - 0 (Viral Structural Proteins)
RN  - EC 2.7.7.7 (DNA-Directed DNA Polymerase)
SB  - IM
GS  - pol
MH  - Amino Acid Sequence
MH  - Base Sequence
MH  - Blotting, Southern
MH  - Chromosome Mapping
MH  - Codon
MH  - DNA, Viral/genetics
MH  - DNA-Directed DNA Polymerase/*genetics
MH  - *Genes, Viral
MH  - Genomic Library
MH  - Herpesvirus 6, Human/*genetics
MH  - Molecular Sequence Data
MH  - Polymerase Chain Reaction
MH  - Restriction Mapping
MH  - Viral Structural Proteins/*genetics
PMC - PMC248922
EDAT- 1991/09/01 00:00
MHDA- 1991/09/01 00:01
PMCR- 1991/09/01
CRDT- 1991/09/01 00:00
PHST- 1991/09/01 00:00 [pubmed]
PHST- 1991/09/01 00:01 [medline]
PHST- 1991/09/01 00:00 [entrez]
PHST- 1991/09/01 00:00 [pmc-release]
AID - 10.1128/JVI.65.9.4670-4680.1991 [doi]
PST - ppublish
SO  - J Virol. 1991 Sep;65(9):4670-80. doi: 10.1128/JVI.65.9.4670-4680.1991.