PMID- 16514174
OWN - NLM
STAT- MEDLINE
DCOM- 20060403
LR  - 20190608
IS  - 1535-3702 (Print)
IS  - 1535-3699 (Linking)
VI  - 231
IP  - 3
DP  - 2006 Mar
TI  - Obesity-induced hypertension develops in young rats independently of the 
      renin-angiotensin-aldosterone system.
PG  - 282-7
AB  - A correlation exists between obesity and hypertension. In the currently available 
      models of diet-induced obesity, the treatment of rats with a high fat (HF) diet 
      does not begin until adulthood. Our aim was to develop and characterize a model 
      of pre-pubescent obesity-induced hypertension. Male Sprague-Dawley rats were fed 
      a HF diet (35% fat) for 10 weeks, beginning at age 3 weeks. Blood pressure was 
      measured by tail-cuff, and a terminal blood sample was obtained to measure 
      fasting blood glucose, insulin, plasma renin, aldosterone, thiobarbitutic acid 
      reactive substances (TBARS), and free 8-isoprostanes levels. The vascular 
      reactivity in the aorta was assessed using a myograph. Blood pressure was 
      increased in rats fed the HF diet (HF, 161 +/- 2 mm Hg vs. control, 137 +/- 2 mm 
      Hg, P < 0.05). Blood glucose (HF, 155 +/- 4 mg/dL vs. control, 123 +/- 5 mg/dL, P 
      < 0.05), insulin (HF, 232 +/- 63 pM vs. control, 60 +/- 11 pM, P < 0.05), TBARS 
      (expressed as nM of malondialdehyde [MDA]/ml [HF, 1.8 +/- 0.37 nM MDA/ml vs. 
      control 1.05 +/- 0.09 nM MDA/ml, P < 0.05]), and free 8-isoprostanes (HF, 229 +/- 
      68 pg/ml vs. control, 112 +/- 9 pg/ml, P < 0.05) levels were elevated in the HF 
      diet group. Interestingly, plasma renin and aldosterone levels were not different 
      between the groups. The maximum vasoconstriction to phenylephrine (10(-4) M) was 
      increased in the HF diet group (HF, 26.1 +/- 1.5 mN vs. control 22.3 +/- 1.2 mN, 
      P < 0.05). In conclusion, pre-pubescent rats become hypertensive and have 
      increased oxidative stress and enhanced vasoconstriction when fed a HF diet. 
      Surprisingly, this occurs without the increase in renin or aldosterone levels 
      seen in the adult models of diet-induced obesity.
FAU - Smith, Anita D
AU  - Smith AD
AD  - Department of Physiology, Medical College of Georgia, Augusta, 30912-3000, USA. 
      asmithgs@students.mcg.edu
FAU - Brands, Michael W
AU  - Brands MW
FAU - Wang, Mong-Heng
AU  - Wang MH
FAU - Dorrance, Anne M
AU  - Dorrance AM
LA  - eng
GR  - HL077385/HL/NHLBI NIH HHS/United States
GR  - HL56259/HL/NHLBI NIH HHS/United States
GR  - HL70887/HL/NHLBI NIH HHS/United States
PT  - Journal Article
PT  - Research Support, N.I.H., Extramural
PT  - Research Support, Non-U.S. Gov't
PL  - Switzerland
TA  - Exp Biol Med (Maywood)
JT  - Experimental biology and medicine (Maywood, N.J.)
JID - 100973463
RN  - 0 (Blood Glucose)
RN  - 0 (Dietary Fats)
RN  - 0 (Insulin)
RN  - 0 (Thiobarbituric Acid Reactive Substances)
RN  - 1WS297W6MV (Phenylephrine)
RN  - 4964P6T9RB (Aldosterone)
RN  - EC 3.4.23.15 (Renin)
RN  - N9YNS0M02X (Acetylcholine)
SB  - IM
MH  - Acetylcholine/pharmacology
MH  - Aging/blood
MH  - Aldosterone/blood
MH  - Animals
MH  - Blood Glucose
MH  - Cardiovascular System/drug effects/*physiopathology
MH  - Dietary Fats/administration & dosage
MH  - Disease Models, Animal
MH  - Heart/anatomy & histology
MH  - Hypertension/*etiology/*physiopathology
MH  - Insulin/blood
MH  - Intra-Abdominal Fat/pathology
MH  - Male
MH  - Obesity/*physiopathology
MH  - Organ Size
MH  - Oxidative Stress
MH  - Phenylephrine/pharmacology
MH  - Rats
MH  - Rats, Sprague-Dawley
MH  - Renin/blood
MH  - Renin-Angiotensin System/*physiology
MH  - Thiobarbituric Acid Reactive Substances/analysis
MH  - Vasoconstriction/drug effects
EDAT- 2006/03/04 09:00
MHDA- 2006/04/04 09:00
CRDT- 2006/03/04 09:00
PHST- 2006/03/04 09:00 [pubmed]
PHST- 2006/04/04 09:00 [medline]
PHST- 2006/03/04 09:00 [entrez]
AID - 231/3/282 [pii]
AID - 10.1177/153537020623100307 [doi]
PST - ppublish
SO  - Exp Biol Med (Maywood). 2006 Mar;231(3):282-7. doi: 10.1177/153537020623100307.