PMID- 16514201
OWN - NLM
STAT- MEDLINE
DCOM- 20060919
LR  - 20231213
IS  - 1470-1626 (Print)
IS  - 1470-1626 (Linking)
VI  - 131
IP  - 3
DP  - 2006 Mar
TI  - Comparison of the expression of human leukocyte antigen (HLA)-G and HLA-E in 
      women with normal pregnancy and those with recurrent miscarriage.
PG  - 583-9
AB  - Recurrent miscarriage affects 1% of all couples attempting pregnancy. 
      Immunological factors are postulated to play a role in the aetiology of recurrent 
      miscarriage because the fetus and placenta are immunologically different from the 
      mother. In particular, altered expression of the, non-classical, class I 
      histocompatibility leukocyte antigen (HLA) molecules has been postulated to play 
      a role in the aetiology of recurrent miscarriage as the fetus and placenta are 
      semi-allogenic to the mother. This study was conducted to examine whether altered 
      expression of the non-classical class I HLA molecules, HLA-G and HLA-E, by cells 
      at the maternofetal interface could play a role in the aetiology of recurrent 
      miscarriage. First-trimester placental and decidual biopsies were obtained from 
      45 women with recurrent miscarriage and 17 gestation-matched normal controls. 
      These biopsies were screened by immunohistochemistry for HLA-G and HLA-E and 
      isotype-matched control antibodies. Staining was analysed by light microscopy and 
      digital image analysis. In both recurrent miscarriage and normal pregnancy, HLA-G 
      was localised to the extravillous trophoblast. There was no difference in the 
      pattern of HLA-G expression between women with recurrent miscarriage and those 
      with normal pregnancies. HLA-E was localised to the syncytiotrophoblast, villous 
      mesenchymal cells, extravillous trophoblast and several decidual cell types, but 
      staining for HLA-E appeared to be confined primarily to the cytoplasm. There was 
      no difference in the pattern of HLA-E expression between women with recurrent 
      miscarriage and those with normal pregnancies.
FAU - Bhalla, A
AU  - Bhalla A
AD  - Department of Obstetrics and Gynaecology, Room 3241, Faculty of Medical and 
      Health Sciences, University of Auckland, Private Bag 92019, Auckland, New 
      Zealand.
FAU - Stone, P R
AU  - Stone PR
FAU - Liddell, H S
AU  - Liddell HS
FAU - Zanderigo, A
AU  - Zanderigo A
FAU - Chamley, L W
AU  - Chamley LW
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - England
TA  - Reproduction
JT  - Reproduction (Cambridge, England)
JID - 100966036
RN  - 0 (HLA Antigens)
RN  - 0 (HLA-G Antigens)
RN  - 0 (Histocompatibility Antigens Class I)
SB  - IM
MH  - Abortion, Habitual/*immunology
MH  - Adult
MH  - Case-Control Studies
MH  - Decidua/*immunology
MH  - Female
MH  - HLA Antigens/*analysis
MH  - HLA-G Antigens
MH  - Histocompatibility Antigens Class I/*analysis
MH  - Humans
MH  - Immunohistochemistry/methods
MH  - Placenta/*immunology
MH  - Pregnancy
MH  - Pregnancy Trimester, First
MH  - HLA-E Antigens
EDAT- 2006/03/04 09:00
MHDA- 2006/09/20 09:00
CRDT- 2006/03/04 09:00
PHST- 2006/03/04 09:00 [pubmed]
PHST- 2006/09/20 09:00 [medline]
PHST- 2006/03/04 09:00 [entrez]
AID - 131/3/583 [pii]
AID - 10.1530/rep.1.00892 [doi]
PST - ppublish
SO  - Reproduction. 2006 Mar;131(3):583-9. doi: 10.1530/rep.1.00892.