PMID- 1651426
OWN - NLM
STAT- MEDLINE
DCOM- 19910919
LR  - 20200724
IS  - 0022-538X (Print)
IS  - 1098-5514 (Electronic)
IS  - 0022-538X (Linking)
VI  - 65
IP  - 9
DP  - 1991 Sep
TI  - Human herpesvirus 6 induces interleukin-1 beta and tumor necrosis factor alpha, 
      but not interleukin-6, in peripheral blood mononuclear cell cultures.
PG  - 5105-10
AB  - The human herpesvirus 6 (HHV-6) is known to interact intimately with cells of the 
      immune system. Here we report that HHV-6 is a potent inducer of interleukin-1 
      beta (IL-1 beta) and tumor necrosis factor alpha (TNF-alpha) in cultures of 
      peripheral blood mononuclear cells. In contradistinction, HHV-6 has no effect on 
      IL-6 synthesis. Maximal IL-1 beta and TNF-alpha gene transcription, as detected 
      by polymerase chain reaction amplification analysis, is observed at 12 and 6 h 
      postinfection, respectively. Release of IL-1 beta and TNF-alpha into the culture 
      supernatants peaked at 24 h and gradually decreased with time. Heat-inactivated 
      virus was unable to stimulate IL-1 beta and TNF-alpha syntheses, whereas 
      UV-irradiated virus retained the full monokine-inducing potential of the native 
      particle. Preincubation of viral preparation with neutralizing anti-HHV-6 
      antibody resulted in the abrogation of this cytokine-inducing effect, whereas 
      treatment of cells with phosphonoacetic acid (an inhibitor of viral DNA 
      polymerase activity) had no effect on the ability of the virus to stimulate 
      monokine release. These results indicate that HHV-6 can exert a strong 
      immunomodulatory effect by stimulating the cells of myeloid lineage to produce 
      these cytokines.
FAU - Flamand, L
AU  - Flamand L
AD  - Laboratory of Immunovirology, Faculty of Medicine, University of Montreal, 
      Quebec, Canada.
FAU - Gosselin, J
AU  - Gosselin J
FAU - D'Addario, M
AU  - D'Addario M
FAU - Hiscott, J
AU  - Hiscott J
FAU - Ablashi, D V
AU  - Ablashi DV
FAU - Gallo, R C
AU  - Gallo RC
FAU - Menezes, J
AU  - Menezes J
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - J Virol
JT  - Journal of virology
JID - 0113724
RN  - 0 (Interleukin-1)
RN  - 0 (Interleukin-6)
RN  - 0 (Oligonucleotides)
RN  - 0 (RNA, Messenger)
RN  - 0 (Tumor Necrosis Factor-alpha)
SB  - IM
MH  - Base Sequence
MH  - Cells, Cultured
MH  - Gene Expression
MH  - Herpesviridae Infections/*physiopathology
MH  - Herpesvirus 6, Human/*physiology
MH  - Humans
MH  - In Vitro Techniques
MH  - Interleukin-1/*biosynthesis/genetics
MH  - Interleukin-6/*biosynthesis
MH  - Leukocytes, Mononuclear/*microbiology
MH  - Molecular Sequence Data
MH  - Oligonucleotides/chemistry
MH  - Polymerase Chain Reaction
MH  - RNA, Messenger/genetics
MH  - Tumor Necrosis Factor-alpha/*biosynthesis/genetics
PMC - PMC248979
EDAT- 1991/09/01 00:00
MHDA- 1991/09/01 00:01
PMCR- 1991/09/01
CRDT- 1991/09/01 00:00
PHST- 1991/09/01 00:00 [pubmed]
PHST- 1991/09/01 00:01 [medline]
PHST- 1991/09/01 00:00 [entrez]
PHST- 1991/09/01 00:00 [pmc-release]
AID - 10.1128/JVI.65.9.5105-5110.1991 [doi]
PST - ppublish
SO  - J Virol. 1991 Sep;65(9):5105-10. doi: 10.1128/JVI.65.9.5105-5110.1991.