PMID- 16516498
OWN - NLM
STAT- MEDLINE
DCOM- 20061006
LR  - 20061124
IS  - 1053-8119 (Print)
IS  - 1053-8119 (Linking)
VI  - 31
IP  - 3
DP  - 2006 Jul 1
TI  - In vivo imaging with cellular resolution of bone marrow cells transplanted into 
      the ischemic brain of a mouse.
PG  - 958-67
AB  - The aim of the study was to monitor in vivo and noninvasively the fate of single 
      bone marrow cells (BMCs) transplanted into the ischemic brain of unirradiated 
      mice. In vivo imaging was performed through a closed cranial window, throughout 
      the 2 weeks following cell transplantation, using laser-scanning confocal 
      fluorescence microscopy. The window was chronically implanted above the left 
      parieto-occipital cortex in C57BL/6J adult mice. BMC (3 x 10(5) nucleated cells 
      in 0.5 microL medium) from 5-week-old transgenic mice, ubiquitously expressing 
      green fluorescent protein (GFP), was transplanted into the ipsilateral cortex 24 
      h after the induction of focal ischemia by coagulation of the left middle 
      cerebral artery (n = 15). Three nonischemic mice served as controls. Repeated in 
      vivo imaging, up to a depth of 200 microm, revealed that BMCs survived within the 
      ischemic and peri-ischemic cortex, migrated significantly towards the lesion, 
      proliferated and adopted a microglia-like morphology over 2 weeks. These results 
      were confirmed using ex vivo imaging after appropriate immunocytochemical 
      treatments. This study indicates that confocal fluorescence microscopy is a 
      reliable and unique tool to repeatedly assess with cellular resolution the in 
      vivo dynamic fate of fluorescent cells transplanted into a mouse brain. These 
      results also provide the first in vivo findings on the fate of single BMCs 
      transplanted into the ischemic brain of unirradiated mice.
FAU - Tran-Dinh, Alexy
AU  - Tran-Dinh A
AD  - Cardiovascular Research Centre, INSERM U 689, Universite Paris 7, 10 Avenue de 
      Verdun, 75010 Paris, France.
FAU - Kubis, Nathalie
AU  - Kubis N
FAU - Tomita, Yutaka
AU  - Tomita Y
FAU - Karaszewski, Bartosz
AU  - Karaszewski B
FAU - Calando, Yolande
AU  - Calando Y
FAU - Oudina, Karim
AU  - Oudina K
FAU - Petite, Herve
AU  - Petite H
FAU - Seylaz, Jacques
AU  - Seylaz J
FAU - Pinard, Elisabeth
AU  - Pinard E
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20060303
PL  - United States
TA  - Neuroimage
JT  - NeuroImage
JID - 9215515
RN  - 147336-22-9 (Green Fluorescent Proteins)
SB  - IM
EIN - Neuroimage. 2006 Nov 15;33(3):1028. Dinh, Alexy Tran [corrected to Tran-Dinh, 
      Alexy]
MH  - Animals
MH  - Bone Marrow Cells/*pathology
MH  - Bone Marrow Transplantation/*pathology
MH  - Brain Ischemia/*pathology
MH  - Cell Division/physiology
MH  - Cell Movement/physiology
MH  - Cell Survival/physiology
MH  - Cerebral Cortex/blood supply/pathology
MH  - Green Fluorescent Proteins/analysis
MH  - *Image Processing, Computer-Assisted
MH  - Infarction, Middle Cerebral Artery/*pathology
MH  - *Laser Scanning Cytometry
MH  - Mice
MH  - Mice, Inbred C57BL
MH  - Microglia/pathology
EDAT- 2006/03/07 09:00
MHDA- 2006/10/07 09:00
CRDT- 2006/03/07 09:00
PHST- 2005/09/27 00:00 [received]
PHST- 2006/01/05 00:00 [revised]
PHST- 2006/01/12 00:00 [accepted]
PHST- 2006/03/07 09:00 [pubmed]
PHST- 2006/10/07 09:00 [medline]
PHST- 2006/03/07 09:00 [entrez]
AID - S1053-8119(06)00044-9 [pii]
AID - 10.1016/j.neuroimage.2006.01.019 [doi]
PST - ppublish
SO  - Neuroimage. 2006 Jul 1;31(3):958-67. doi: 10.1016/j.neuroimage.2006.01.019. Epub 
      2006 Mar 3.