PMID- 16516503
OWN - NLM
STAT- MEDLINE
DCOM- 20060606
LR  - 20181201
IS  - 1556-7117 (Electronic)
IS  - 1071-5576 (Linking)
VI  - 13
IP  - 3
DP  - 2006 Apr
TI  - An in vitro coculture model to study cytokine profiles of natural killer cells 
      during maternal immune cell-trophoblast interactions.
PG  - 196-202
AB  - OBJECTIVES: The cytokine milieu at the implantation site plays a role in human 
      pregnancy. Th2 cytokines, such as interleukin (IL)-4 and IL-10, stimulate growth 
      and development of placenta, whereas Th1 cytokines, such as tumor necrosis 
      factor-alpha (TNF-alpha), are associated with pregnancy complications. Natural 
      killer (NK) cells predominate at the implantation site. The aim of the present 
      study is to investigate cytokine expression in NK cells when they are in close 
      contact with JEG-3 trophoblast-like cells using an in vitro coculture model. 
      METHODS: Female peripheral blood mononuclear cells (PBMCs) were cocultured with 
      JEG-3 cells for 24 hours. PBMCs were harvested from the cocultures and stimulated 
      with 25 ng/mL phorbol myristate acetate and 1 micromol/mL ionomycin in the 
      presence of 2 micromol/mL monensin. NK cells were analyzed by flow cytometry for 
      intracellular TNF-alpha, interferon-gamma (IFN-gamma), and IL-4 and IL-10 
      cytokines. Controls were PBMCs cultured without JEG-3 cells. RESULTS: The 
      proportion of CD56+/TNF-alpha(+) NK cells was significantly decreased when they 
      were in coculture with JEG-3 cells (26.1%) as compared to without JEG-3 cell 
      coculture (40.8%) (P < .05). There was no difference in the proportion of CD56(+) 
      NK cells expressing intracellular IFN-gamma, IL-4, and IL-10. Down-regulation of 
      CD56+/TNF-alpha(+) NK cell levels was dependent on direct cell-to-cell contact 
      between NK cells and JEG-3 cells. The expression of human leukocyte antigen 
      (HLA)-G on trophoblast cell lines did not affect CD56+/TNF-alpha(+) NK cell 
      levels under these experimental conditions. CONCLUSION: We report that JEG-3 
      cells induce down-regulation of intracellular CD56+/TNF-alpha(+) NK cell levels. 
      It is speculated that trophoblasts may secure themselves from NK cell 
      cytotoxicity via this mechanism.
FAU - Ntrivalas, Evangelos
AU  - Ntrivalas E
AD  - Department of Microbiology and Immunology, Rosalind Franklin University of 
      Medicine and Science, Chicago, Illinois 60064, USA.
FAU - Kwak-Kim, Joanne
AU  - Kwak-Kim J
FAU - Beaman, Kenneth
AU  - Beaman K
FAU - Mantouvalos, Harilaos
AU  - Mantouvalos H
FAU - Gilman-Sachs, Alice
AU  - Gilman-Sachs A
LA  - eng
PT  - Journal Article
DEP - 20060303
PL  - United States
TA  - J Soc Gynecol Investig
JT  - Journal of the Society for Gynecologic Investigation
JID - 9433806
RN  - 0 (Cytokines)
RN  - 0 (HLA Antigens)
RN  - 0 (HLA-G Antigens)
RN  - 0 (Histocompatibility Antigens Class I)
SB  - IM
MH  - Cell Communication
MH  - Cell Culture Techniques
MH  - Cytokines/immunology/*metabolism
MH  - Down-Regulation
MH  - Embryo Implantation
MH  - Female
MH  - Flow Cytometry
MH  - HLA Antigens/immunology
MH  - HLA-G Antigens
MH  - Histocompatibility Antigens Class I/immunology
MH  - Humans
MH  - Infant, Newborn
MH  - Killer Cells, Natural/*immunology
MH  - Leukocytes, Mononuclear
MH  - Pregnancy/*immunology
MH  - Trophoblasts/*immunology
EDAT- 2006/03/07 09:00
MHDA- 2006/06/07 09:00
CRDT- 2006/03/07 09:00
PHST- 2005/10/31 00:00 [received]
PHST- 2006/03/07 09:00 [pubmed]
PHST- 2006/06/07 09:00 [medline]
PHST- 2006/03/07 09:00 [entrez]
AID - S1071-5576(05)00416-8 [pii]
AID - 10.1016/j.jsgi.2005.12.009 [doi]
PST - ppublish
SO  - J Soc Gynecol Investig. 2006 Apr;13(3):196-202. doi: 10.1016/j.jsgi.2005.12.009. 
      Epub 2006 Mar 3.