PMID- 16517015
OWN - NLM
STAT- MEDLINE
DCOM- 20060720
LR  - 20131121
IS  - 0196-9781 (Print)
IS  - 0196-9781 (Linking)
VI  - 27
IP  - 5
DP  - 2006 May
TI  - Neuropeptide FF (NPFF) control of magnocellular neurosecretory cells of the rat 
      hypothalamic paraventricular nucleus (PVN).
PG  - 973-9
AB  - Neuropeptide FF (NPFF) is an octapeptide belonging to an extended family of RF 
      amide peptides that have been implicated in a wide variety of physiological 
      functions in the brain. NPFF and its receptors are abundantly expressed in the 
      rat brain and spinal cord including the hypothalamic paraventricular nucleus 
      (PVN), an autonomic nucleus critical for the secretion of neurohormones and the 
      regulation of sympathetic outflow. In this study, we sought to examine the 
      effects of NPFF on GABAergic inhibitory synaptic input to magnocellular 
      neurosecretory cells (MNCs) of the PVN, which secrete the neurohormones, 
      vasopressin and oxytocin from their terminals in the neurohypophysis. Whole cell 
      patch clamp recordings under voltage clamp conditions were performed from PVN 
      MNCs in the brain slice. Bicuculline-sensitive inhibitory postsynaptic currents 
      (IPSCs) were isolated in the presence of glutamate receptor blockers. In 
      tetrodotoxin, NPFF (5 microM) caused an increase in frequency, but not amplitude 
      of miniature inhibitory postsynaptic currents (mIPSCs) in MNCs indicating a 
      presynaptic locus of action for this peptide. Intracerebroventricular application 
      of NPFF resulted in an activation of GABAergic neurons located adjacent to the 
      PVN as revealed by immunohistochemistry for Fos protein and in situ hybridization 
      for glutamic acid decarboxylase (GAD67) mRNA. Based on these observations we 
      conclude that NPFF facilitates inhibitory input to MNCs of the PVN via GABAergic 
      interneurons located in immediate vicinity of the nucleus. These findings provide 
      a cellular and anatomic basis for the NPFF-induced inhibition of vasopressin 
      release has been reported consequent to hypovolemia and hyperosmolar stimulation.
FAU - Jhamandas, Jack H
AU  - Jhamandas JH
AD  - Department of Medicine (Neurology) and Centre for Neuroscience, University of 
      Alberta, Edmonton, Alta., Canada T6G 2B7. jack.jhamandas@ualberta.ca
FAU - MacTavish, David
AU  - MacTavish D
FAU - Harris, Kim H
AU  - Harris KH
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20060306
PL  - United States
TA  - Peptides
JT  - Peptides
JID - 8008690
RN  - 0 (Isoenzymes)
RN  - 0 (Oligopeptides)
RN  - 0 (Proto-Oncogene Proteins c-fos)
RN  - 0 (Receptors, GABA-A)
RN  - 4368-28-9 (Tetrodotoxin)
RN  - 99566-27-5 
      (phenylalanyl-leucyl-phenylalanyl-glutaminyl-prolyl-glutaminyl-arginyl-phenylalaninamide)
RN  - EC 4.1.1.15 (Glutamate Decarboxylase)
RN  - EC 4.1.1.15 (glutamate decarboxylase 1)
RN  - H030S2S85J (Kynurenic Acid)
RN  - Y37615DVKC (Bicuculline)
SB  - IM
MH  - Animals
MH  - Bicuculline/pharmacology
MH  - Excitatory Postsynaptic Potentials/drug effects
MH  - Glutamate Decarboxylase/biosynthesis
MH  - Injections, Intraventricular
MH  - Isoenzymes/biosynthesis
MH  - Kynurenic Acid/pharmacology
MH  - Male
MH  - Neurosecretory Systems/cytology/*metabolism
MH  - Oligopeptides/pharmacology/*physiology
MH  - Paraventricular Hypothalamic Nucleus/*cytology/physiology
MH  - Patch-Clamp Techniques
MH  - Proto-Oncogene Proteins c-fos/biosynthesis
MH  - Rats
MH  - Rats, Sprague-Dawley
MH  - Receptors, GABA-A/drug effects/physiology
MH  - Tetrodotoxin/pharmacology
EDAT- 2006/03/07 09:00
MHDA- 2006/07/21 09:00
CRDT- 2006/03/07 09:00
PHST- 2005/03/31 00:00 [received]
PHST- 2005/07/06 00:00 [accepted]
PHST- 2006/03/07 09:00 [pubmed]
PHST- 2006/07/21 09:00 [medline]
PHST- 2006/03/07 09:00 [entrez]
AID - S0196-9781(06)00037-4 [pii]
AID - 10.1016/j.peptides.2005.07.030 [doi]
PST - ppublish
SO  - Peptides. 2006 May;27(5):973-9. doi: 10.1016/j.peptides.2005.07.030. Epub 2006 
      Mar 6.