PMID- 16517499
OWN - NLM
STAT- MEDLINE
DCOM- 20060510
LR  - 20131121
IS  - 1071-5762 (Print)
IS  - 1029-2470 (Linking)
VI  - 40
IP  - 4
DP  - 2006 Apr
TI  - Broad-spectrum antioxidant peptides derived from His residue-containing sequences 
      present in human paraoxonase 1.
PG  - 349-58
AB  - Hydroxyl or peroxyl radicals and hypochlorous acid (HOCl) are known to cause the 
      oxidation of lipoproteins. Here, we examined Cu(2+)-binding property of 
      paraoxonase 1 (PON1), and antioxidant actions of peptides, resembling His 
      residue-containing sequences in PON1, against oxidations by Cu(2+), peroxyl 
      radicals or HOCl. When Cu(2+)-binding property of PON1 was examined 
      spectrophotometrically, the maximal Cu(2+) binding was achieved at 1:1 molar 
      ratio of PON1: Cu(2+). Additionally, Cu(2+)-catalyzed oxidative inactivation of 
      PON1 was prevented by Ca(2+)-depleted PON1 at 1:1 ratio, but not 
      diethylpyrocarbonate (DEPC)-modified PON1, suggesting the participation of His 
      residue in Cu(2+)-binding. When His-containing peptides were examined for 
      antioxidant actions, those with either His residue at N-terminal position 2 or 3, 
      or His-Pro sequence at C-terminal remarkably prevented Cu(2+)-mediated low 
      density lipoprotein (LDL) oxidation and PON1 inactivation. Especially, FHKALY, 
      FHKY or NHP efficiently prevented Cu(2+)-induced LDL oxidation (24 h), indicating 
      a tight binding of Cu(2+) by peptides. In support of this, the peptide/Cu(2+) 
      complexes exhibited a superoxide-scavenging activity. Separately, in oxidations 
      by 2,2'-azobis-2-amidinopropane hydrochloride or HOCl, the presence of Tyrosine 
      (Tyr) or Cysteine (Cys) residue markedly enhanced antioxidant action of 
      His-containing peptides. These results indicate that His-containing peptides with 
      Tys or Cys residues correspond to broad spectrum antioxidants in oxidation models 
      employing Cu(2+), 2,2'-azobis-2-amidinopropane hydrochloride (AAPH) or HOCl.
FAU - Nguyen, Su Duy
AU  - Nguyen SD
AD  - College of Pharmacy, Chungnam National University, Yuseong-Ku, Taejon 305 764, 
      South Korea.
FAU - Jeong, Tae-Sook
AU  - Jeong TS
FAU - Kim, Mee Ree
AU  - Kim MR
FAU - Sok, Dai-Eun
AU  - Sok DE
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - England
TA  - Free Radic Res
JT  - Free radical research
JID - 9423872
RN  - 0 (Antioxidants)
RN  - 0 (Lipoproteins)
RN  - 0 (Oxidants)
RN  - 0 (Peptides)
RN  - 0 (Peroxides)
RN  - 3170-83-0 (perhydroxyl radical)
RN  - 4QD397987E (Histidine)
RN  - 789U1901C5 (Copper)
RN  - EC 3.1.8.1 (Aryldialkylphosphatase)
RN  - EC 3.1.8.1 (PON1 protein, human)
SB  - IM
MH  - Antioxidants/*chemical synthesis/chemistry/metabolism
MH  - Aryldialkylphosphatase/*chemistry/metabolism
MH  - Copper/metabolism
MH  - Histidine/*chemistry
MH  - Humans
MH  - Lipoproteins/metabolism
MH  - Oxidants/metabolism
MH  - Oxidation-Reduction
MH  - Peptides/*chemical synthesis/*chemistry/metabolism
MH  - Peroxides/metabolism
EDAT- 2006/03/07 09:00
MHDA- 2006/05/11 09:00
CRDT- 2006/03/07 09:00
PHST- 2006/03/07 09:00 [pubmed]
PHST- 2006/05/11 09:00 [medline]
PHST- 2006/03/07 09:00 [entrez]
AID - H13U20870KKU3658 [pii]
AID - 10.1080/10715760500534429 [doi]
PST - ppublish
SO  - Free Radic Res. 2006 Apr;40(4):349-58. doi: 10.1080/10715760500534429.