PMID- 16517660
OWN - NLM
STAT- MEDLINE
DCOM- 20060530
LR  - 20240314
IS  - 0099-2240 (Print)
IS  - 1098-5336 (Electronic)
IS  - 0099-2240 (Linking)
VI  - 72
IP  - 3
DP  - 2006 Mar
TI  - Phylogenetic analysis and in situ identification of bacteria community 
      composition in an acidic Sphagnum peat bog.
PG  - 2110-7
AB  - The Bacteria community composition in an acidic Sphagnum peat bog (pH 3.9 to 4.5) 
      was characterized by a combination of 16S rRNA gene clone library analysis, 
      rRNA-targeted fluorescence in situ hybridization (FISH), and cultivation. Among 
      84 environmental 16S rRNA gene clones, a set of only 16 cloned sequences was 
      closely related (>or=95% similarity) to taxonomically described organisms. Main 
      groups of clones were affiliated with the Acidobacteria (24 clones), 
      Alphaproteobacteria (20), Verrucomicrobia (13), Actinobacteria (8), 
      Deltaproteobacteria (4), Chloroflexi (3), and Planctomycetes (3). The proportion 
      of cells that hybridized with oligonucleotide probes specific for members of the 
      domains Bacteria (EUB338-mix) and Archaea (ARCH915 and ARC344) accounted for only 
      12 to 22% of the total cell counts. Up to 24% of the EUB338-positive cells could 
      be assigned by FISH to specific bacterial phyla. Alphaproteobacteria and 
      Planctomycetes were the most numerous bacterial groups (up to 1.3x10(7) and 
      1.1x10(7) cells g-1 peat, respectively). In contrast to conventional plating 
      techniques, a novel biofilm-mediated enrichment approach allowed us to isolate 
      some representatives of predominant Bacteria groups, such as Acidobacteria and 
      Planctomycetes. This novel strategy has great potential to enable the isolation 
      of a significant proportion of the peat bog bacterial diversity.
FAU - Dedysh, Svetlana N
AU  - Dedysh SN
AD  - S. N. Winogradsky Institute of Microbiology, Russian Academy of Sciences, Moscow, 
      Russia.
FAU - Pankratov, Timofei A
AU  - Pankratov TA
FAU - Belova, Svetlana E
AU  - Belova SE
FAU - Kulichevskaya, Irina S
AU  - Kulichevskaya IS
FAU - Liesack, Werner
AU  - Liesack W
LA  - eng
SI  - GENBANK/AM162403
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PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Appl Environ Microbiol
JT  - Applied and environmental microbiology
JID - 7605801
RN  - 0 (Culture Media)
RN  - 0 (DNA, Ribosomal)
RN  - 0 (RNA, Ribosomal, 16S)
SB  - IM
MH  - Bacteria/*classification/genetics/growth & development
MH  - Culture Media
MH  - DNA, Ribosomal/analysis
MH  - *Ecosystem
MH  - Gene Library
MH  - Genes, rRNA
MH  - Hydrogen-Ion Concentration
MH  - In Situ Hybridization, Fluorescence
MH  - Molecular Sequence Data
MH  - *Phylogeny
MH  - RNA, Ribosomal, 16S
MH  - Sequence Analysis, DNA
MH  - *Soil Microbiology
MH  - *Sphagnopsida
PMC - PMC1393241
EDAT- 2006/03/07 09:00
MHDA- 2006/05/31 09:00
PMCR- 2006/07/01
CRDT- 2006/03/07 09:00
PHST- 2006/03/07 09:00 [pubmed]
PHST- 2006/05/31 09:00 [medline]
PHST- 2006/03/07 09:00 [entrez]
PHST- 2006/07/01 00:00 [pmc-release]
AID - 72/3/2110 [pii]
AID - 2135-05 [pii]
AID - 10.1128/AEM.72.3.2110-2117.2006 [doi]
PST - ppublish
SO  - Appl Environ Microbiol. 2006 Mar;72(3):2110-7. doi: 
      10.1128/AEM.72.3.2110-2117.2006.