PMID- 16517725
OWN - NLM
STAT- MEDLINE
DCOM- 20060424
LR  - 20190516
IS  - 0022-1767 (Print)
IS  - 0022-1767 (Linking)
VI  - 176
IP  - 6
DP  - 2006 Mar 15
TI  - Fms-like tyrosine kinase 3 ligand recruits plasmacytoid dendritic cells to the 
      brain.
PG  - 3566-77
AB  - The lack of professional afferent APCs in naive brain parenchyma contributes to 
      the systemic immune ignorance to Ags localized exclusively within the brain. 
      Dendritic cells (DCs) appear within the brain as a consequence of inflammation, 
      but no molecular mechanisms accounting for this influx have been described. In 
      this study we demonstrate that Fms-like tyrosine kinase 3 ligand (Flt3L) recruits 
      plasmacytoid DCs (pDCs; >50-fold; p < 0.001) to the brain parenchyma. These pDCs 
      expressed IFN-alpha, the hallmark cytokine produced by pDCs, indicating 
      recruitment and activation in situ of bona fide pDCs within the brain parenchyma. 
      Flt3L did not increase the numbers of conventional DCs, macrophages, or B, T, NK, 
      NKT, or microglial cells within the brain. Our data demonstrate that Flt3L 
      reconstitutes a crucial afferent component of the immune response, namely, 
      professional APCs within the brain parenchyma, and this could counteract the 
      intrinsic systemic immune ignorance to Ags localized exclusively within the 
      brain.
FAU - Curtin, James F
AU  - Curtin JF
AD  - Gene Therapeutics Research Institute, Cedars Sinai Medical Center, 8700 Beverly 
      Boulevard, Los Angeles, CA 90048, USA.
FAU - King, Gwendalyn D
AU  - King GD
FAU - Barcia, Carlos
AU  - Barcia C
FAU - Liu, Chunyan
AU  - Liu C
FAU - Hubert, Francois X
AU  - Hubert FX
FAU - Guillonneau, Carole
AU  - Guillonneau C
FAU - Josien, Regis
AU  - Josien R
FAU - Anegon, Ignacio
AU  - Anegon I
FAU - Lowenstein, Pedro R
AU  - Lowenstein PR
FAU - Castro, Maria G
AU  - Castro MG
LA  - eng
GR  - 1R03 TW 006273-01/TW/FIC NIH HHS/United States
GR  - R21 NS 047298-01/NS/NINDS NIH HHS/United States
GR  - R21 NS047298/NS/NINDS NIH HHS/United States
GR  - R01 NS044556/NS/NINDS NIH HHS/United States
GR  - U54 NS045309/NS/NINDS NIH HHS/United States
GR  - 1R01 NS 42893-01/NS/NINDS NIH HHS/United States
GR  - U54 NS 045309-01/NS/NINDS NIH HHS/United States
GR  - R01 NS042893/NS/NINDS NIH HHS/United States
GR  - R03 TW006273/TW/FIC NIH HHS/United States
GR  - 1R01 NS 44556-01/NS/NINDS NIH HHS/United States
PT  - Journal Article
PT  - Research Support, N.I.H., Extramural
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - J Immunol
JT  - Journal of immunology (Baltimore, Md. : 1950)
JID - 2985117R
RN  - 0 (Biomarkers)
RN  - 0 (Interferon-alpha)
RN  - 0 (Ligands)
RN  - 0 (TLR7 protein, rat)
RN  - 0 (Toll-Like Receptor 7)
RN  - EC 2.7.10.1 (fms-Like Tyrosine Kinase 3)
SB  - IM
MH  - Animals
MH  - B-Lymphocytes/metabolism
MH  - Biomarkers
MH  - Brain/*cytology/*metabolism
MH  - Cell Movement
MH  - Cell Separation
MH  - Dendritic Cells/*cytology/*metabolism
MH  - Gene Expression Regulation/genetics
MH  - Humans
MH  - Interferon-alpha/genetics/metabolism
MH  - Ligands
MH  - Male
MH  - Organ Specificity
MH  - Rats
MH  - Toll-Like Receptor 7/genetics/metabolism
MH  - fms-Like Tyrosine Kinase 3/genetics/*metabolism
PMC - PMC1592227
MID - NIHMS11836
COIS- Disclosures The authors have no financial conflict of interest.
EDAT- 2006/03/07 09:00
MHDA- 2006/04/25 09:00
PMCR- 2008/02/01
CRDT- 2006/03/07 09:00
PHST- 2006/03/07 09:00 [pubmed]
PHST- 2006/04/25 09:00 [medline]
PHST- 2006/03/07 09:00 [entrez]
PHST- 2008/02/01 00:00 [pmc-release]
AID - 176/6/3566 [pii]
AID - 10.4049/jimmunol.176.6.3566 [doi]
PST - ppublish
SO  - J Immunol. 2006 Mar 15;176(6):3566-77. doi: 10.4049/jimmunol.176.6.3566.