PMID- 16518539
OWN - NLM
STAT- MEDLINE
DCOM- 20060602
LR  - 20190513
IS  - 1672-9145 (Print)
IS  - 1672-9145 (Linking)
VI  - 38
IP  - 3
DP  - 2006 Mar
TI  - Induction of the Epstein-Barr virus latent membrane protein 2 antigen-specific 
      cytotoxic T lymphocytes using human leukocyte antigen tetramer-based artificial 
      antigen-presenting cells.
PG  - 157-63
AB  - Cytotoxic T lymphocytes (CTLs) specific for the Epstein-Barr virus (EBV) latent 
      membrane protein 2 (LMP2) antigen are important reagents for the treatment of 
      some EBV-associated malignancies, such as EBV-positive Hodgkin's disease and 
      nasopharyngeal carcinoma. However, the therapeutic amount of CTLs is often 
      hampered by the limited supply of antigen-presenting cells. To address this 
      issue, an artificial antigen-presenting cell (aAPC) was made by coating a human 
      leukocyte antigen (HLA)-pLMP2 tetrameric complex, anti-CD28 antibody and CD54 
      molecule to a cell-sized latex bead, which provided the dual signals required for 
      T cell activation. By co-culture of the HLA-A2-LMP2 bearing aAPC and peripheral 
      blood mononuclear cells from HLA-A2 positive healthy donors, LMP2 
      antigen-specific CTLs were induced and expanded in vitro. The specificity of the 
      aAPC-induced CTLs was demonstrated by both HLA-A2-LMP2 tetramer staining and 
      cytotoxicity against HLA-A2-LMP2 bearing T2 cell, the cytotoxicity was inhibited 
      by the anti-HLA class I antibody (W6/32). These results showed that LMP2 
      antigen-specific CTLs could be induced and expanded in vitro by the 
      HLA-A2-LMP2-bearing aAPC. Thus, aAPCs coated with an HLA-pLMP2 complex, anti-CD28 
      and CD54 might be promising tools for the enrichment of LMP2-specific CTLs for 
      adoptive immunotherapy.
FAU - Lu, Xiao-Ling
AU  - Lu XL
AD  - Department of Immunology, Tongji Medical College, Huazhong University of Science 
      and Technology, Wuhan 430030, China.
FAU - Liang, Zhi-Hui
AU  - Liang ZH
FAU - Zhang, Cai-E
AU  - Zhang CE
FAU - Lu, Sheng-Jun
AU  - Lu SJ
FAU - Weng, Xiu-Fang
AU  - Weng XF
FAU - Wu, Xiong-Wen
AU  - Wu XW
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - China
TA  - Acta Biochim Biophys Sin (Shanghai)
JT  - Acta biochimica et biophysica Sinica
JID - 101206716
RN  - 0 (EBV-associated membrane antigen, Epstein-Barr virus)
RN  - 0 (HLA Antigens)
RN  - 0 (HLA-A2 Antigen)
RN  - 0 (Viral Matrix Proteins)
RN  - 126547-89-5 (Intercellular Adhesion Molecule-1)
SB  - IM
MH  - Antigen-Presenting Cells/cytology/*metabolism
MH  - CD4-Positive T-Lymphocytes/immunology/metabolism
MH  - Epstein-Barr Virus Infections/*drug therapy
MH  - HLA Antigens/chemistry/*metabolism
MH  - HLA-A2 Antigen/immunology
MH  - Hodgkin Disease/drug therapy/immunology/pathology
MH  - Humans
MH  - Intercellular Adhesion Molecule-1/immunology/metabolism
MH  - Leukocytes/chemistry/*immunology
MH  - Nasopharyngeal Neoplasms/drug therapy/immunology/pathology
MH  - T-Lymphocytes, Cytotoxic/*immunology
MH  - Tumor Cells, Cultured
MH  - Viral Matrix Proteins/*immunology
EDAT- 2006/03/07 09:00
MHDA- 2006/06/03 09:00
CRDT- 2006/03/07 09:00
PHST- 2006/03/07 09:00 [pubmed]
PHST- 2006/06/03 09:00 [medline]
PHST- 2006/03/07 09:00 [entrez]
AID - 10.1111/j.1745-7270.2006.00150.x [doi]
PST - ppublish
SO  - Acta Biochim Biophys Sin (Shanghai). 2006 Mar;38(3):157-63. doi: 
      10.1111/j.1745-7270.2006.00150.x.