PMID- 16518880
OWN - NLM
STAT- MEDLINE
DCOM- 20060719
LR  - 20061115
IS  - 1099-498X (Print)
IS  - 1099-498X (Linking)
VI  - 8
IP  - 6
DP  - 2006 Jun
TI  - Photochemical treatment with endosomally localized photosensitizers enhances the 
      number of adenoviruses in the nucleus.
PG  - 707-18
AB  - BACKGROUND: In the present study the physical targeting technique photochemical 
      internalization (PCI) has been used in combination with adenovirus. We have 
      previously shown that PCI enhances transgene expression from AdhCMV-lacZ, and the 
      aim of the present study was to further increase the understanding of 
      photochemically mediated adenoviral transduction. METHODS: Two colorectal 
      carcinoma cell lines, WiDr and HCT116, were pre-incubated with the 
      photosensitizer TPPS(2a) or methylene blue derivates (MBD) followed by infection 
      with adenovirus and light exposure. Transgene expression was measured by flow 
      cytometry. Real-time polymerase chain reaction (PCR) and fluorescence in situ 
      hybridization (FISH) were used to quantify the level of viral DNA in the nuclei. 
      Real-time PCR was also used to measure the level of beta-galactosidase mRNA in 
      samples infected with AdhCMV-lacZ. RESULTS: Exposing TPPS(2a)-treated cells to 
      light enhanced the quantity of viral DNA in the nucleus, the mRNA level of the 
      transgene and the transgene expression compared to non-illuminated cells. The 
      increased transgene expression was independent of the promoter used, but 
      dependent on the time of light exposure and the cellular localization of the 
      photosensitizer. CONCLUSIONS: The enhanced transgene expression observed after 
      photochemical treatment is most likely not a result of one event, but more an 
      interplay between various mechanisms. An increased level of adenoviral DNA in the 
      nucleus and a dependency of endosomal localization of the photosensitizer to 
      obtain enhanced transgene expression suggested that endosomal rupture facilitated 
      the transport of adenoviruses to the nucleus.
CI  - Copyright 2006 John Wiley & Sons, Ltd.
FAU - Engesaeter, Birgit O
AU  - Engesaeter BO
AD  - Department of Tumor Biology, Rikshospitalet-Radiumhospitalet Health Enterprise, 
      Montebello, N-0310 Oslo, Norway. birgite@labmed.uio.no
FAU - Tveito, Siri
AU  - Tveito S
FAU - Bonsted, Anette
AU  - Bonsted A
FAU - Engebraaten, Olav
AU  - Engebraaten O
FAU - Berg, Kristian
AU  - Berg K
FAU - Maelandsmo, Gunhild M
AU  - Maelandsmo GM
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - England
TA  - J Gene Med
JT  - The journal of gene medicine
JID - 9815764
RN  - 0 (DNA, Viral)
RN  - 0 (Photosensitizing Agents)
RN  - 0 (RNA, Messenger)
RN  - 0 (enhanced green fluorescent protein)
RN  - 147336-22-9 (Green Fluorescent Proteins)
RN  - EC 3.2.1.23 (beta-Galactosidase)
SB  - IM
MH  - Adenoviridae/*drug effects/genetics/*isolation & purification
MH  - Cell Line, Tumor
MH  - Cell Nucleus/*drug effects/*radiation effects/virology
MH  - DNA, Viral/genetics
MH  - Endosomes/*metabolism
MH  - Gene Expression/radiation effects
MH  - Genome, Viral/genetics
MH  - Green Fluorescent Proteins/genetics
MH  - HCT116 Cells
MH  - Humans
MH  - Photosensitizing Agents/metabolism/*pharmacology
MH  - RNA, Messenger/genetics/metabolism
MH  - Time Factors
MH  - Transduction, Genetic
MH  - Transgenes
MH  - Tumor Cells, Cultured
MH  - beta-Galactosidase/genetics
EDAT- 2006/03/07 09:00
MHDA- 2006/07/20 09:00
CRDT- 2006/03/07 09:00
PHST- 2006/03/07 09:00 [pubmed]
PHST- 2006/07/20 09:00 [medline]
PHST- 2006/03/07 09:00 [entrez]
AID - 10.1002/jgm.902 [doi]
PST - ppublish
SO  - J Gene Med. 2006 Jun;8(6):707-18. doi: 10.1002/jgm.902.