PMID- 16519699
OWN - NLM
STAT- MEDLINE
DCOM- 20060505
LR  - 20161124
IS  - 0902-4441 (Print)
IS  - 0902-4441 (Linking)
VI  - 76
IP  - 4
DP  - 2006 Apr
TI  - New chromosome abnormalities and lack of BCL-6 gene rearrangements in Argentinean 
      diffuse large B-cell lymphomas.
PG  - 284-93
AB  - OBJECTIVES: Diffuse large B-cell lymphoma (DLBCL) is the most common form of 
      non-Hodgkin lymphomas. Cytogenetic studies have revealed a broad spectrum of 
      clonal genetic abnormalities and complex karyotypes. The purpose of this study 
      was to contribute to the understanding of the genomic alterations associated with 
      this group of lymphomas. METHODS: Cytogenetic, fluorescence in situ hybridization 
      (FISH) and molecular analyses were performed in 30 cases with DLBCL: 20 de novo 
      DLBCL (dn-DLBCL) and 10 DLBCL secondary to follicular lymphoma (S-DLBCL). 
      RESULTS: A total of 37 different structural chromosomal rearrangements were 
      found: 27% translocations, 54% deletions, and 19% other alterations. Chromosomes 
      8, 6, 2, and 9 were the most commonly affected. Interestingly, translocation 
      t(3;14)(q27;q32) and/or BCL-6 gene rearrangements were not observed either by 
      cytogenetic studies or by FISH analysis. Fifteen novel cytogenetic alterations 
      were detected, among them translocations t(2;21)(p11;q22) and t(8;18)(q24;p11.3) 
      appeared as sole structural abnormalities. Translocation t(14;18)(q32;q21) and/or 
      BCL-2-IGH gene rearrangements were the genomic alterations most frequently 
      observed: 50% of S-DLBCL and 30% of dn-DLBCL. Deletions del(4)(q21), del(6)(q27), 
      del(8)(q11), and del(9)(q11) were recurrent. The most common gains involved 
      chromosome regions at 12q13-q24, 7q10-q32, and 17q22-qter; 6q was the most 
      frequently deleted region, followed by losses at 2q35-qter, 7q32-qter, and 
      9q13-qter. Four novel regions of loss were identified: 5q13-q21, 2q35-qter (both 
      recurrent in our series), 4p11-p12, and 17q11-q12. CONCLUSIONS: These studies 
      emphasize the value of combining conventional cytogenetics with FISH and 
      molecular studies to allow a more accurate definition of the genomic aberrations 
      involved in DLBCL.
FAU - Cerretini, Roxana
AU  - Cerretini R
AD  - Departamento de Genetica, Instituto de Investigaciones Hematologicas Mariano R. 
      Castex, Academia Nacional de Medicina, Buenos Aires, Argentina. 
      rcerretini@argentina.com
FAU - Noriega, Maria F
AU  - Noriega MF
FAU - Narbaitz, Marina
AU  - Narbaitz M
FAU - Slavutsky, Irma
AU  - Slavutsky I
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - England
TA  - Eur J Haematol
JT  - European journal of haematology
JID - 8703985
RN  - 0 (BCL6 protein, human)
RN  - 0 (DNA-Binding Proteins)
RN  - 0 (Proto-Oncogene Proteins c-bcl-6)
SB  - IM
MH  - Adolescent
MH  - Adult
MH  - Aged
MH  - Argentina
MH  - Chromosomes, Human/*genetics
MH  - DNA-Binding Proteins/genetics
MH  - Female
MH  - Humans
MH  - In Situ Hybridization, Fluorescence/methods
MH  - Karyotyping
MH  - Lymphoma, B-Cell/*genetics/pathology
MH  - Lymphoma, Follicular/*genetics/pathology
MH  - Lymphoma, Large B-Cell, Diffuse/*genetics/pathology
MH  - Male
MH  - Middle Aged
MH  - Neoplasms, Second Primary/*genetics/pathology
MH  - Proto-Oncogene Proteins c-bcl-6
MH  - Sequence Deletion/genetics
MH  - Translocation, Genetic/*genetics
EDAT- 2006/03/08 09:00
MHDA- 2006/05/06 09:00
CRDT- 2006/03/08 09:00
PHST- 2006/03/08 09:00 [pubmed]
PHST- 2006/05/06 09:00 [medline]
PHST- 2006/03/08 09:00 [entrez]
AID - EJH616 [pii]
AID - 10.1111/j.1600-0609.2005.00616.x [doi]
PST - ppublish
SO  - Eur J Haematol. 2006 Apr;76(4):284-93. doi: 10.1111/j.1600-0609.2005.00616.x.