PMID- 16519931
OWN - NLM
STAT- MEDLINE
DCOM- 20060829
LR  - 20131121
IS  - 0141-8130 (Print)
IS  - 0141-8130 (Linking)
VI  - 38
IP  - 2
DP  - 2006 Mar 30
TI  - Synthesis of tentacle-type magnetic beads as immobilized metal-chelate affinity 
      support for cytochrome c adsorption.
PG  - 126-33
AB  - Magnetic poly(2-hydroxyethylmethacrylate) (mPHEMA) beads with an average diameter 
      of 100-140 microm were produced by suspension polymerization in the presence of 
      magnetite particles (i.e. Fe3O4). Specific surface area and average pore size of 
      the magnetic beads was found to be 50 m2/g and 819 nm, respectively. Ester groups 
      in the mPHEMA structure were converted to imine groups by reacting with 
      poly(ethyleneimine) (PEI) in the presence of NaH. Amino (-NH2) content of 
      PEI-attached mPHEMA beads was determined as 102 mg PEI/g. Then, Cu2+ ions were 
      chelated on the magnetic beads in the range of 20-793 micromol Cu2+/g. Cytochrome 
      c (cyt c) adsorption was performed on the metal chelating beads from aqueous 
      solutions containing different amounts of cyt c at different pHs, Cu2+ loadings 
      and temperatures. Cyt c adsorption on the mPHEMA/PEI beads was 4.6 mg/g. Cu2+ 
      chelation increased the cyt c adsorption significantly (40.1 mg/g). Adsorption 
      capacity increased with Cu2+ loading and then reached a saturation value. Cyt c 
      adsorption decreased with increasing temperature. Cyt c molecules could be 
      reversibly adsorbed and eluted ten times with the magnetic adsorbents without 
      noticeable loss in their cyt c adsorption capacity. The applicability of two 
      kinetic models including pseudo-first order and pseudo-second order model was 
      estimated on the basis of comparative analysis of the corresponding rate 
      parameters, equilibrium capacity and correlation coefficients. Results suggest 
      that chemisorption processes could be the rate-limiting step in the adsorption 
      process. In the last part of this article, cyt c adsorption experiments were 
      performed in a magnetically stabilized fluidized bed (MSFB) system at optimum 
      conditions determined from the batch experiments. The adsorption capacity 
      decreased significantly from 46.8 to 15.4 mg/g polymer with the increase of the 
      flow-rate from 0.5 to 4.0 ml/min. The resulting magnetic chelator beads possessed 
      excellent long-term storage stability.
FAU - Turkmen, Deniz
AU  - Turkmen D
AD  - Department of Chemistry, Hacettepe University, Ankara, Turkey.
FAU - Yavuz, Handan
AU  - Yavuz H
FAU - Denizli, Adil
AU  - Denizli A
LA  - eng
PT  - Journal Article
DEP - 20060307
PL  - Netherlands
TA  - Int J Biol Macromol
JT  - International journal of biological macromolecules
JID - 7909578
RN  - 0 (Chelating Agents)
RN  - 0 (Hydrogels)
RN  - 0 (Imines)
RN  - 0 (Polyamines)
RN  - 0 (Polyethylenes)
RN  - 0 (Solutions)
RN  - 0 (poly(2-hydroxyethyl methacrylate)-polyamine graft copolymer)
RN  - 0 (poly(ethylene imine))
RN  - 059QF0KO0R (Water)
RN  - 25249-16-5 (Polyhydroxyethyl Methacrylate)
RN  - 789U1901C5 (Copper)
RN  - 9007-43-6 (Cytochromes c)
RN  - XM0M87F357 (Ferrosoferric Oxide)
SB  - IM
MH  - Adsorption
MH  - Animals
MH  - Chelating Agents/*chemistry
MH  - Chromatography, Affinity/*methods
MH  - *Copper
MH  - Cytochromes c/*isolation & purification
MH  - Ferrosoferric Oxide
MH  - Horses
MH  - Hydrogels
MH  - Hydrogen-Ion Concentration
MH  - Imines/chemistry
MH  - *Immunomagnetic Separation
MH  - *Microspheres
MH  - Mitochondria, Heart/enzymology
MH  - Particle Size
MH  - Polyamines/*chemistry
MH  - Polyethylenes/chemistry
MH  - Polyhydroxyethyl Methacrylate/*analogs & derivatives/chemistry
MH  - Porosity
MH  - Solutions
MH  - Surface Properties
MH  - Water
EDAT- 2006/03/08 09:00
MHDA- 2006/08/30 09:00
CRDT- 2006/03/08 09:00
PHST- 2005/11/07 00:00 [received]
PHST- 2006/01/26 00:00 [revised]
PHST- 2006/01/26 00:00 [accepted]
PHST- 2006/03/08 09:00 [pubmed]
PHST- 2006/08/30 09:00 [medline]
PHST- 2006/03/08 09:00 [entrez]
AID - S0141-8130(06)00038-9 [pii]
AID - 10.1016/j.ijbiomac.2006.01.018 [doi]
PST - ppublish
SO  - Int J Biol Macromol. 2006 Mar 30;38(2):126-33. doi: 
      10.1016/j.ijbiomac.2006.01.018. Epub 2006 Mar 7.