PMID- 16520180
OWN - NLM
STAT- MEDLINE
DCOM- 20060407
LR  - 20121003
IS  - 0741-5214 (Print)
IS  - 0741-5214 (Linking)
VI  - 43
IP  - 3
DP  - 2006 Mar
TI  - Monocyte recruitment in venous thrombus resolution.
PG  - 601-8
AB  - OBJECTIVE: To investigate the importance of monocyte recruitment in thrombus 
      resolution and the role of cysteine-cysteine (CC) chemokines and the CC chemokine 
      receptor, CCR2, in this process. METHODS: Peritoneal macrophages, monocyte 
      chemotactic protein 1 (MCP1), or carrier solutions were injected into thrombi 
      induced in the vena cava of rats. Caval thrombi were also formed in CCR2-/- and 
      MCP1-/- mice and in wild-type mice transfected with an adenoviral construct 
      expressing a broad-spectrum CC receptor antagonist. RESULTS: Direct 
      administration of peritoneal macrophages decreased thrombus size by more than 
      fivefold and increased recanalization by more than fourfold compared with 
      controls (P < .001). A 100-ng MCP1dose reduced thrombus size by more than sixfold 
      (P < .01) and increased recanalization by more than sevenfold (P < .01), without 
      affecting macrophage recruitment. Deletion of CCR2 or blockade of all CC 
      chemokines inhibited both monocyte recruitment (P < .05) and thrombus resolution 
      (P < .01), but knocking out MCP-1 had no effect. CONCLUSION: Increasing 
      macrophage numbers in the thrombus enhances its resolution. MCP1 treatment 
      enhances resolution by stimulating recanalization, independent of an effect on 
      monocyte recruitment. CCR2 deficiency has the same effect as blockade of all CC 
      chemokines. CCR2 receptor activation may therefore be an important mechanism in 
      monocyte recruitment into venous thrombi and could be targeted to promote their 
      resolution.
FAU - Ali, Tahir
AU  - Ali T
AD  - Academic Department of Surgery, Cardiovascular Division, GKT, King's College, St 
      Thomas's Hospital, London, United Kingdom.
FAU - Humphries, Julia
AU  - Humphries J
FAU - Burnand, Kevin
AU  - Burnand K
FAU - Sawyer, Barbara
AU  - Sawyer B
FAU - Bursill, Christina
AU  - Bursill C
FAU - Channon, Keith
AU  - Channon K
FAU - Greaves, David
AU  - Greaves D
FAU - Rollins, Barrett
AU  - Rollins B
FAU - Charo, Israel F
AU  - Charo IF
FAU - Smith, Alberto
AU  - Smith A
LA  - eng
GR  - HL063894/HL/NHLBI NIH HHS/United States
GR  - HL52773/HL/NHLBI NIH HHS/United States
PT  - Journal Article
PT  - Research Support, N.I.H., Extramural
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - J Vasc Surg
JT  - Journal of vascular surgery
JID - 8407742
RN  - 0 (Ccr2 protein, mouse)
RN  - 0 (Ccr2 protein, rat)
RN  - 0 (Chemokine CCL2)
RN  - 0 (Receptors, CCR2)
RN  - 0 (Receptors, Chemokine)
SB  - IM
MH  - Animals
MH  - Cell Count
MH  - Chemokine CCL2/analysis/physiology
MH  - Macrophages/physiology
MH  - Mice
MH  - Monocytes/*physiology
MH  - Peritoneal Lavage
MH  - Rats
MH  - Receptors, CCR2
MH  - Receptors, Chemokine/analysis/physiology
MH  - Venous Thrombosis/pathology/*physiopathology
EDAT- 2006/03/08 09:00
MHDA- 2006/04/08 09:00
CRDT- 2006/03/08 09:00
PHST- 2005/06/28 00:00 [received]
PHST- 2005/10/18 00:00 [accepted]
PHST- 2006/03/08 09:00 [pubmed]
PHST- 2006/04/08 09:00 [medline]
PHST- 2006/03/08 09:00 [entrez]
AID - S0741-5214(05)01924-5 [pii]
AID - 10.1016/j.jvs.2005.10.073 [doi]
PST - ppublish
SO  - J Vasc Surg. 2006 Mar;43(3):601-8. doi: 10.1016/j.jvs.2005.10.073.