PMID- 16520552
OWN - NLM
STAT- MEDLINE
DCOM- 20060420
LR  - 20131121
IS  - 1226-3613 (Print)
IS  - 1226-3613 (Linking)
VI  - 38
IP  - 1
DP  - 2006 Feb 28
TI  - Chronic mild stress decreases survival, but not proliferation, of new-born cells 
      in adult rat hippocampus.
PG  - 44-54
AB  - New-born cells continue to proliferate and survive to become mature granule cells 
      in adult rat hippocampus. Although this process, known as neurogenesis, is 
      inhibited by acute stress, it is not clear whether chronic stress affects 
      neurogenesis. To determine whether chronic mild stress (CMS) influences 
      neurogenesis in the adult rat hippocampus, male Sprague-Dawley rats were exposed 
      to CMS and administered bromodeoxyuridine (BrdU) before or after CMS to observe 
      the survival/differentiation or proliferation of new-born cells, respectively. In 
      addition, we measured brain-derived neurotrophic factor (BDNF) mRNA in the 
      granule cell layer (GCL) of the hippocampus, because BDNF is known to play an 
      important role in the survival of new-born cells. CMS significantly decreased the 
      survival of new-born cells in the GCL, but did not influence the proliferation or 
      differentiation of new-born cells. CMS did not affect the proliferation and 
      survival of new-born cells in the hilus. In addition, CMS did not change BDNF 
      mRNA levels in the GCL. These results demonstrate that CMS reduces the survival 
      of new-born cells but not of their proliferation, suggesting that repeated mild 
      stress could influence a part of neurogenesis, but not the whole part of 
      neurogenesis. These results raise the possibility that the survival of new-born 
      cells may be suppressed in the presence of normal BDNF mRNA levels in GCL.
FAU - Lee, Kuem-Ju
AU  - Lee KJ
AD  - Graduate School of Biomedical Sciences, Korea University College of Medicine, 
      Seoul 136-705, Korea.
FAU - Kim, Sung-Jin
AU  - Kim SJ
FAU - Kim, Suk-Won
AU  - Kim SW
FAU - Choi, Song-Hyen
AU  - Choi SH
FAU - Shin, You-Chan
AU  - Shin YC
FAU - Park, Sang-Ha
AU  - Park SH
FAU - Moon, Bo-Hyun
AU  - Moon BH
FAU - Cho, Eujin
AU  - Cho E
FAU - Lee, Min-Soo
AU  - Lee MS
FAU - Choi, Sang-Hyun
AU  - Choi SH
FAU - Chun, Boe-Gwun
AU  - Chun BG
FAU - Shin, Kyung-Ho
AU  - Shin KH
LA  - eng
PT  - Comparative Study
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Exp Mol Med
JT  - Experimental & molecular medicine
JID - 9607880
RN  - 0 (Brain-Derived Neurotrophic Factor)
RN  - 0 (Calbindins)
RN  - 0 (Fluorescent Dyes)
RN  - 0 (Glial Fibrillary Acidic Protein)
RN  - 0 (RNA, Messenger)
RN  - 0 (Rhodamines)
RN  - 0 (S100 Calcium Binding Protein G)
RN  - G34N38R2N1 (Bromodeoxyuridine)
RN  - I223NX31W9 (Fluorescein-5-isothiocyanate)
SB  - IM
MH  - Animals
MH  - Brain-Derived Neurotrophic Factor/metabolism
MH  - Bromodeoxyuridine/*administration & dosage
MH  - Calbindins
MH  - Cell Proliferation
MH  - Cell Survival
MH  - Fluorescein-5-isothiocyanate
MH  - Fluorescent Antibody Technique, Indirect
MH  - Fluorescent Dyes
MH  - Glial Fibrillary Acidic Protein/metabolism
MH  - Hippocampus/cytology/growth & development/*pathology
MH  - Immunohistochemistry
MH  - In Situ Hybridization
MH  - Male
MH  - Microscopy, Confocal
MH  - RNA, Messenger/metabolism
MH  - Rats
MH  - Rats, Sprague-Dawley
MH  - Restraint, Physical
MH  - Rhodamines
MH  - S100 Calcium Binding Protein G/metabolism
MH  - Stress, Physiological/pathology/*physiopathology
EDAT- 2006/03/08 09:00
MHDA- 2006/04/21 09:00
CRDT- 2006/03/08 09:00
PHST- 2006/03/08 09:00 [pubmed]
PHST- 2006/04/21 09:00 [medline]
PHST- 2006/03/08 09:00 [entrez]
AID - 200602286 [pii]
AID - 10.1038/emm.2006.6 [doi]
PST - ppublish
SO  - Exp Mol Med. 2006 Feb 28;38(1):44-54. doi: 10.1038/emm.2006.6.