PMID- 16520657
OWN - NLM
STAT- MEDLINE
DCOM- 20061204
LR  - 20190917
IS  - 0959-4973 (Print)
IS  - 0959-4973 (Linking)
VI  - 17
IP  - 3
DP  - 2006 Mar
TI  - Cytogenetic toxicity of cycloplatam in human lymphocytes: detection by the 
      micronucleus test and fluorescence in situ hybridization.
PG  - 289-95
AB  - Cycloplatam has been shown to be effective in the treatment of pleural 
      mesothelioma, myeloma and ovarian carcinoma. Cycloplatam is not nephrotoxic with 
      respect to the platinum-based anti-tumor agents. We have investigated the 
      mechanism underlying the induction of micronuclei (MN) in human lymphocytes by 
      cycloplatam compared to that by its parent drugs cisplatin and carboplatin. The 
      cytokinesis-block micronucleus assay in human lymphocytes was applied in 
      combination with fluorescence in situ hybridization (FISH) with an all-chromosome 
      centromeric probe allowing discrimination between MN due to chromosomal fragments 
      (centromere negative, C) and those containing whole chromosomes (centromere 
      positive, C). A statistically significant increase of MN frequency (P<0.001) was 
      detected for cisplatin, carboplatin and cycloplatam. However, cycloplatam was 
      active at a much lower dose (0.1 micromol/l) than cisplatin or carboplatin (1 
      micromol/l). No significant increase in the frequency of C or C MN was observed 
      for cisplatin and carboplatin compared to the controls. A statistically 
      significant (P<0.001) increase in the percentage of C MN was observed in 
      cycloplatam-treated cells. The results obtained suggest different mechanisms for 
      cytogenetic damage induced by platinum drugs. Cycloplatam induces one type of MN 
      and it could be considered a clastogenic agent, whereas cisplatin and carboplatin 
      appear to induce both chromosome breakage and numerical chromosomal 
      abnormalities.
FAU - Nersesyan, Armen
AU  - Nersesyan A
AD  - Cancer Research Center, Yerevan, Armenia.
FAU - Perrone, Emanuela
AU  - Perrone E
FAU - Roggieri, Paola
AU  - Roggieri P
FAU - Bolognesi, Claudia
AU  - Bolognesi C
LA  - eng
PT  - Journal Article
PL  - England
TA  - Anticancer Drugs
JT  - Anti-cancer drugs
JID - 9100823
RN  - 0 (Organoplatinum Compounds)
RN  - 109837-67-4 (cycloplatam)
RN  - BG3F62OND5 (Carboplatin)
RN  - Q20Q21Q62J (Cisplatin)
SB  - IM
MH  - Carboplatin/pharmacology
MH  - Cisplatin/pharmacology
MH  - Female
MH  - Humans
MH  - In Situ Hybridization, Fluorescence
MH  - Lymphocytes/*drug effects
MH  - Male
MH  - Micronuclei, Chromosome-Defective/*chemically induced
MH  - Micronucleus Tests
MH  - Organoplatinum Compounds/*pharmacology
EDAT- 2006/03/08 09:00
MHDA- 2006/12/09 09:00
CRDT- 2006/03/08 09:00
PHST- 2006/03/08 09:00 [pubmed]
PHST- 2006/12/09 09:00 [medline]
PHST- 2006/03/08 09:00 [entrez]
AID - 00001813-200603000-00007 [pii]
AID - 10.1097/00001813-200603000-00007 [doi]
PST - ppublish
SO  - Anticancer Drugs. 2006 Mar;17(3):289-95. doi: 10.1097/00001813-200603000-00007.