PMID- 16521274
OWN - NLM
STAT- MEDLINE
DCOM- 20060410
LR  - 20191210
IS  - 0090-3493 (Print)
IS  - 0090-3493 (Linking)
VI  - 34
IP  - 3
DP  - 2006 Mar
TI  - A novel adsorbent of circulating bacterial toxins and cytokines: the effect of 
      direct hemoperfusion with CTR column for the treatment of experimental 
      endotoxemia.
PG  - 800-6
AB  - OBJECTIVES: The current study examined the ability of a new adsorbent, CTR, to 
      remove enterotoxins, toxic shock syndrome toxin-1 (TSST-1), and cytokines from 
      blood and/or serum in vitro and the effects of the extracorporeal treatment with 
      CTR column on mortality rate and inflammatory responses to endotoxic shock in 
      vivo. DESIGN: Laboratory investigation. SETTING: University and company 
      experimental laboratory. MATERIALS: CTR is composed of porous cellulose beads to 
      which a hydrophobic organic compound with a hexadecyl alkyl chain has been 
      covalently bound to the surface as a ligand. Human/bovine serum and human blood 
      samples in vitro and Male Wistar rats were used. INTERVENTIONS: CTR's ability to 
      adsorb bacterial toxins and cytokines related to sepsis in serum and/or blood was 
      examined with an in vitro batch adsorption protocol. In vivo, male Wistar rats 
      were anesthetized and assigned to one of three groups (n=14 per group): 
      Escherichia coli endotoxin (15 mg/kg intravenously) alone (endotoxemic), 
      apheresis with control column without CTR for 120 mins (control column), or 
      extracorporeal treatment with CTR column for 120 mins (CTR treatment). 
      MEASUREMENTS AND MAIN RESULTS: With use of the CTR adsorbent, the adsorption 
      rates were 50% to 90% for enterotoxins, TSST-1, and cytokines such as TNF-alpha 
      and interleukin (IL)-6 in the batch tests. In vivo, the mortality rates at 8 hrs 
      after endotoxin injection were 92%, 92%, and 14% for the endotoxemic, control 
      column, and CTR treatment groups, respectively. Hypotension and elevated plasma 
      cytokine concentrations and the infiltration of neutrophils of the lungs were 
      less conspicuous in the CTR treatment group than in the other two groups. 
      CONCLUSIONS: CTR, a novel adsorbent, effectively adsorbed small- to middle-sized 
      proteins, such as cytokines, enterotoxins, and TSST-1 in vitro. Direct 
      hemoperfusion apheresis with CTR column reduced mortality and had inhibitory 
      effects on the inflammatory responses during endotoxemia in vivo. These findings 
      suggest that extracorporeal blood purification with CTR column may be available 
      to use for patients with sepsis and/or endotoxemia.
FAU - Taniguchi, Takumi
AU  - Taniguchi T
AD  - Department of Emergency and Critical Care Medicine, Graduate School of Medical 
      Science, Kanazawa University, Japan. takutaniyan@yahoo.co.jp
FAU - Hirai, Fumiyasu
AU  - Hirai F
FAU - Takemoto, Yasuhiro
AU  - Takemoto Y
FAU - Tsuda, Kazunobu
AU  - Tsuda K
FAU - Yamamoto, Ken
AU  - Yamamoto K
FAU - Inaba, Hideo
AU  - Inaba H
FAU - Sakurai, Hiroshi
AU  - Sakurai H
FAU - Furuyoshi, Shigeo
AU  - Furuyoshi S
FAU - Tani, Nobutaka
AU  - Tani N
LA  - eng
PT  - Evaluation Study
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Crit Care Med
JT  - Critical care medicine
JID - 0355501
RN  - 0 (Bacterial Toxins)
RN  - 0 (Cytokines)
RN  - 0 (Enterotoxins)
RN  - 0 (Superantigens)
RN  - 0 (enterotoxin F, Staphylococcal)
RN  - 9004-34-6 (Cellulose)
SB  - IM
CIN - Crit Care Med. 2006 Mar;34(3):926-7. PMID: 16505690
MH  - Adsorption
MH  - Analysis of Variance
MH  - Animals
MH  - Bacterial Toxins/*pharmacokinetics
MH  - Cellulose/analogs & derivatives/therapeutic use
MH  - Cytokines/*pharmacokinetics
MH  - Enterotoxins/*pharmacokinetics
MH  - Hemoperfusion/*methods
MH  - Humans
MH  - In Vitro Techniques
MH  - Lung/pathology
MH  - Male
MH  - Random Allocation
MH  - Rats
MH  - Rats, Wistar
MH  - Sepsis/therapy
MH  - Shock, Septic/*therapy
MH  - Superantigens
MH  - Survival Analysis
EDAT- 2006/03/08 09:00
MHDA- 2006/04/11 09:00
CRDT- 2006/03/08 09:00
PHST- 2006/03/08 09:00 [pubmed]
PHST- 2006/04/11 09:00 [medline]
PHST- 2006/03/08 09:00 [entrez]
AID - 10.1097/01.ccm.0000202449.15027.ae [doi]
PST - ppublish
SO  - Crit Care Med. 2006 Mar;34(3):800-6. doi: 10.1097/01.ccm.0000202449.15027.ae.