PMID- 16522723
OWN - NLM
STAT- MEDLINE
DCOM- 20060508
LR  - 20061115
IS  - 0022-0795 (Print)
IS  - 0022-0795 (Linking)
VI  - 188
IP  - 3
DP  - 2006 Mar
TI  - Involvement of brain-derived neurotrophic factor in the regulation of 
      hypothalamic somatostatin in vivo.
PG  - 425-33
AB  - Brain-derived neurotrophic factor (BDNF) has been extensively studied in the 
      central nervous system as a survival and differentiation factor and in plasticity 
      processes. In vitro, BDNF has been shown to stimulate cellular differentiation 
      and neurohormones synthesis and release. We demonstrated that BDNF is a potent 
      and specific stimulatory agent of somatostatin (SRIH) synthesis in primary 
      cultures of hypothalamic neurons. However, less information is available about 
      its function on SRIH neurons in vivo. In the present study, we examined the 
      effect of in vivo intracerebroventricular BDNF administration in adult 
      non-anesthetized male rats. Two distinct experimental approaches were used: acute 
      intracerebroventricular injection and long-term (14 days) continuous infusion 
      (Alzet micro-pumps). We demonstrate that single intracerebroventricular BDNF 
      injections (5 microg/rat) induce an early (60 and 180 min) decrease in the SRIH 
      mRNA signal in the hypothalamic periventricular nucleus (PeVN) accompanied by a 
      decrease of the hypothalamic SRIH content. 48 h after the acute injection, SRIH 
      mRNA levels and peptide content strongly and significantly increased. After 
      continuous intracerebroventricular BDNF administration (12 microg/day for 14 
      days), a significant increase in the SRIH hypothalamic content was observed. 
      Nevertheless, the increase in peptide content was not correlated with a similar 
      increase in the PeVN messenger level. These findings show the involvement of BDNF 
      in the in vivo regulation of somatostatinergic neurons in adult rats, which 
      clearly differs according to the BDNF administration mode.
FAU - Givalois, Laurent
AU  - Givalois L
AD  - Molecular Mechanisms in Neurodegenerative Dementia Laboratory, U710 Inserm, 
      University of Montpellier 2, EPHE, Place E Bataillon, 34095 Montpellier, France. 
      lgivalois@univ-montp2.fr
FAU - Naert, Gaelle
AU  - Naert G
FAU - Tapia-Arancibia, Lucia
AU  - Tapia-Arancibia L
FAU - Arancibia, Sandor
AU  - Arancibia S
LA  - eng
PT  - Comparative Study
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - England
TA  - J Endocrinol
JT  - The Journal of endocrinology
JID - 0375363
RN  - 0 (Brain-Derived Neurotrophic Factor)
RN  - 0 (RNA, Messenger)
RN  - 51110-01-1 (Somatostatin)
SB  - IM
MH  - Animals
MH  - Brain-Derived Neurotrophic Factor/*pharmacology
MH  - Dose-Response Relationship, Drug
MH  - Hypothalamus/*chemistry/drug effects
MH  - Image Processing, Computer-Assisted
MH  - In Situ Hybridization/methods
MH  - Infusion Pumps
MH  - Injections, Intraventricular
MH  - Male
MH  - Paraventricular Hypothalamic Nucleus/chemistry/drug effects
MH  - RNA, Messenger/*analysis
MH  - Rats
MH  - Rats, Sprague-Dawley
MH  - Somatostatin/analysis/*genetics
MH  - Stimulation, Chemical
MH  - Time Factors
EDAT- 2006/03/09 09:00
MHDA- 2006/05/09 09:00
CRDT- 2006/03/09 09:00
PHST- 2006/03/09 09:00 [pubmed]
PHST- 2006/05/09 09:00 [medline]
PHST- 2006/03/09 09:00 [entrez]
AID - 188/3/425 [pii]
AID - 10.1677/joe.1.06578 [doi]
PST - ppublish
SO  - J Endocrinol. 2006 Mar;188(3):425-33. doi: 10.1677/joe.1.06578.