PMID- 1652558
OWN - NLM
STAT- MEDLINE
DCOM- 19911002
LR  - 20210526
IS  - 0019-9567 (Print)
IS  - 1098-5522 (Electronic)
IS  - 0019-9567 (Linking)
VI  - 59
IP  - 9
DP  - 1991 Sep
TI  - Regulation of leukotriene B4 generation from human polymorphonuclear granulocytes 
      after stimulation with formyl-methionyl-leucyl phenylalanine: effects of 
      pertussis and cholera toxins.
PG  - 3046-52
AB  - The effects of holotoxins and toxin subunits from Bordetella pertussis and Vibrio 
      cholerae strains on intact and digitonin-permeabilized human polymorphonuclear 
      neutrophils were studied. Our data clearly demonstrate that 
      formyl-methionyl-leucyl-phenylalanine (fMLP)-induced generation of chemotactic 
      active leukotriene B4 was inhibited by both holotoxins as well as by their 
      isolated enzymatic A protomers. In contrast, the respective binding components (B 
      oligomers) did not affect leukotriene formation. Priming of 
      digitonin-permeabilized neutrophils with either guanylylimidodiphosphate or 
      inositol trisphosphate increased subsequent stimulation with fMLP. In contrast, 
      diacylglycerol decreased fMLP-induced leukotriene B4 formation, but inositol 
      trisphosphate and diacylglycerol had no effect on inhibition mediated by the 
      toxins. In addition, pertussis and cholera toxins reduced the specific binding of 
      [3H]fMLP. Scatchard plot analysis revealed that the observed decrease of peptide 
      binding was due to a reduced number of receptor sites. The fMLP-stimulated 
      [3H]guanylylimidodiphosphate binding and GTPase activity used as parameters for 
      the activation of G proteins were decreased in parallel. These results suggest 
      altered chemotactic receptor numbers and G-protein functions responsible for the 
      toxin-dependent suppression of fMLP-mediated response for neutrophils.
FAU - Hensler, T
AU  - Hensler T
AD  - Lehrstuhl fur Medizinische Mikrobiologie und Immunologie, Ruhr-Universitat 
      Bochum, Germany.
FAU - Koller, M
AU  - Koller M
FAU - Konig, W
AU  - Konig W
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Infect Immun
JT  - Infection and immunity
JID - 0246127
RN  - 0 (Phosphatidylinositols)
RN  - 0 (Receptors, Formyl Peptide)
RN  - 0 (Receptors, Immunologic)
RN  - 0 (Virulence Factors, Bordetella)
RN  - 1HGW4DR56D (Leukotriene B4)
RN  - 59880-97-6 (N-Formylmethionine Leucyl-Phenylalanine)
RN  - 9012-63-9 (Cholera Toxin)
RN  - EC 3.6.1.- (GTP-Binding Proteins)
SB  - IM
MH  - Cholera Toxin/*pharmacology
MH  - GTP-Binding Proteins/immunology/metabolism
MH  - Humans
MH  - Leukotriene B4/*immunology
MH  - N-Formylmethionine Leucyl-Phenylalanine/*pharmacology
MH  - Neutrophils/*immunology/metabolism
MH  - Permeability
MH  - Phosphatidylinositols/immunology/metabolism
MH  - Receptors, Formyl Peptide
MH  - Receptors, Immunologic/immunology
MH  - Signal Transduction
MH  - Virulence Factors, Bordetella/*pharmacology
PMC - PMC258133
EDAT- 1991/09/01 00:00
MHDA- 1991/09/01 00:01
PMCR- 1991/09/01
CRDT- 1991/09/01 00:00
PHST- 1991/09/01 00:00 [pubmed]
PHST- 1991/09/01 00:01 [medline]
PHST- 1991/09/01 00:00 [entrez]
PHST- 1991/09/01 00:00 [pmc-release]
AID - 10.1128/iai.59.9.3046-3052.1991 [doi]
PST - ppublish
SO  - Infect Immun. 1991 Sep;59(9):3046-52. doi: 10.1128/iai.59.9.3046-3052.1991.