PMID- 16525638
OWN - NLM
STAT- MEDLINE
DCOM- 20060606
LR  - 20071115
IS  - 1019-6439 (Print)
IS  - 1019-6439 (Linking)
VI  - 28
IP  - 4
DP  - 2006 Apr
TI  - Novel cryptic chromosomal rearrangements detected in acute lymphoblastic leukemia 
      detected by application of new multicolor fluorescent in situ hybridization 
      approaches.
PG  - 891-7
AB  - Routine cytogenetic analysis provides important information on diagnostic and 
      prognostic relevance for hematological malignancies. However, it is often 
      difficult to obtain good karyotypes, especially of cells from cases with acute 
      lymphoblastic leukemia (ALL) because of poor morphology and spreading. Thus, 
      detailed karyotyping can be hampered and even in case of a 'normal karyotype' 
      according to banding cytogenetics doubts remain if the result is reliable. In 
      order to address this problem a series of 37 ALL cases without any detectable 
      numerical or structural chromosomal defects was selected and studied by two 
      recently developed multicolor fluorescence in situ hybridization (FISH) 
      approaches: 1) multitude multicolor banding (mMCB) is a FISH-banding technique, 
      which allows the analyses of inter- and intra-chromosomal rearrangements of the 
      whole human karyotype in one single experiment; 2) chromosome-specific 
      subcentromere/subtelomere-specific multicolor (subCTM-)FISH applies 
      locus-specific subtelomeric and subcentromic probes and enables the 
      characterization of the subtelomeric and peri-centric regions of the chromosomes, 
      not analyzable by other FISH-approaches. Thus, we detected the following 
      recurrent cryptic chromosomal aberrations: del(12)(pter) [8 cases], del(9)(qter) 
      [3 cases], and del(11)(pter) [2 cases]. Moreover, cryptic changes in additional 
      nine subtelomeric and in two subcentromeric regions were observed one time, each. 
      In summary, mMCB and subCTM were proven to be powerful methods in the screening 
      for new cryptic chromosomal aberrations, which considerably increased the 
      accuracy of cytogenetic diagnosis.
FAU - Karst, Constanze
AU  - Karst C
AD  - Institut fur Humangenetik und Anthropologie, D-07743 Jena, Germany.
FAU - Gross, Madeleine
AU  - Gross M
FAU - Haase, Detlef
AU  - Haase D
FAU - Wedding, Ulrich
AU  - Wedding U
FAU - Hoffken, Klaus
AU  - Hoffken K
FAU - Liehr, Thomas
AU  - Liehr T
FAU - Mkrtchyan, Hasmik
AU  - Mkrtchyan H
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - Greece
TA  - Int J Oncol
JT  - International journal of oncology
JID - 9306042
SB  - IM
MH  - Adolescent
MH  - Adult
MH  - Aged
MH  - Child
MH  - Child, Preschool
MH  - *Chromosome Aberrations
MH  - Chromosome Banding/methods
MH  - Female
MH  - Humans
MH  - Male
MH  - Middle Aged
MH  - Precursor Cell Lymphoblastic Leukemia-Lymphoma/*genetics/pathology
MH  - Spectral Karyotyping/*methods
MH  - Telomere/genetics
MH  - Translocation, Genetic/*genetics
EDAT- 2006/03/10 09:00
MHDA- 2006/06/07 09:00
CRDT- 2006/03/10 09:00
PHST- 2006/03/10 09:00 [pubmed]
PHST- 2006/06/07 09:00 [medline]
PHST- 2006/03/10 09:00 [entrez]
PST - ppublish
SO  - Int J Oncol. 2006 Apr;28(4):891-7.