PMID- 16525729
OWN - NLM
STAT- MEDLINE
DCOM- 20060727
LR  - 20181113
IS  - 0920-8569 (Print)
IS  - 0920-8569 (Linking)
VI  - 32
IP  - 1
DP  - 2006 Feb
TI  - Enhanced immunogenicity of the modified GP5 of porcine reproductive and 
      respiratory syndrome virus.
PG  - 5-11
AB  - The ORF5-encoded major envelope glycoprotein (GP5) is one of the key immunogenic 
      proteins of the porcine reproductive and respiratory syndrome virus (PRRSV) and 
      is the leading target for the development of the new generation of vaccines 
      against PRRS. However, weak and tardy neutralizing antibodies have been elicited 
      in several developed experimental vaccines expressing PRRSV GP5. More recent 
      evidence has demonstrated a non-neutralizing decoy epitope upstream of the 
      neutralizing epitope of GP5, which might prevent the development of a strong 
      neutralizing antibody response against PRRSV. In the present study, we modified 
      the ORF5 gene by inserting a Pan DR T-helper cell epitope (PADRE) between the 
      neutralizing epitope and the decoy epitope to minimize or eliminate the decoy 
      effect of the non-neutralizing epitope. The immunogenicity of the modified GP5 
      was further evaluated using DNA vaccination. The results showed that 
      significantly enhanced neutralizing antibodies were elicited in mice immunized 
      with the DNA construct expressing the modified GP5 compared with the native GP5. 
      Slightly increased levels of GP5-specific ELISA antibodies and T-cell 
      proliferative activities were also observed. These results indicate that the high 
      immunogenicity of the modified GP5 might facilitate the development of improved 
      PRRS vaccines in the future.
FAU - Fang, Liurong
AU  - Fang L
AD  - Laboratory of Animal Virology, College of Veterinary Medicine, Huazhong 
      Agricultural University, Wuhan 430070, Hubei, P.R.China.
FAU - Jiang, Yunbo
AU  - Jiang Y
FAU - Xiao, Shaobo
AU  - Xiao S
FAU - Niu, Chuanshuang
AU  - Niu C
FAU - Zhang, Hui
AU  - Zhang H
FAU - Chen, Huanchun
AU  - Chen H
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Virus Genes
JT  - Virus genes
JID - 8803967
RN  - 0 (Antibodies, Viral)
RN  - 0 (Antigens, Viral)
RN  - 0 (DNA, Viral)
RN  - 0 (Immunodominant Epitopes)
RN  - 0 (Plant Proteins)
RN  - 0 (Swin1.1 protein, Salix viminalis)
RN  - 0 (Vaccines, DNA)
RN  - 0 (Viral Envelope Proteins)
RN  - 0 (Viral Vaccines)
RN  - 0 (glycoprotein 5, PRRSV)
SB  - IM
MH  - Amino Acid Sequence
MH  - Animals
MH  - Antibodies, Viral/biosynthesis
MH  - Antigens, Viral/genetics
MH  - Base Sequence
MH  - Cell Line
MH  - DNA, Viral/genetics
MH  - Female
MH  - Genes, Viral
MH  - HeLa Cells
MH  - Humans
MH  - Immunodominant Epitopes/genetics
MH  - Lymphocyte Activation
MH  - Mice
MH  - Mice, Inbred BALB C
MH  - Molecular Sequence Data
MH  - Neutralization Tests
MH  - Open Reading Frames
MH  - Plant Proteins
MH  - Porcine Reproductive and Respiratory Syndrome/immunology/prevention & 
      control/virology
MH  - Porcine respiratory and reproductive syndrome virus/*genetics/*immunology
MH  - Sus scrofa
MH  - Vaccines, DNA/genetics/immunology
MH  - Viral Envelope Proteins/*genetics/*immunology
MH  - Viral Vaccines/genetics/immunology
EDAT- 2006/03/10 09:00
MHDA- 2006/07/28 09:00
CRDT- 2006/03/10 09:00
PHST- 2005/03/24 00:00 [received]
PHST- 2005/05/15 00:00 [accepted]
PHST- 2006/03/10 09:00 [pubmed]
PHST- 2006/07/28 09:00 [medline]
PHST- 2006/03/10 09:00 [entrez]
AID - 10.1007/s11262-005-5839-y [doi]
PST - ppublish
SO  - Virus Genes. 2006 Feb;32(1):5-11. doi: 10.1007/s11262-005-5839-y.