PMID- 16527350
OWN - NLM
STAT- MEDLINE
DCOM- 20070919
LR  - 20131121
IS  - 0145-2126 (Print)
IS  - 0145-2126 (Linking)
VI  - 30
IP  - 7
DP  - 2006 Jul
TI  - Circulating myeloid dendritic cell directly isolated from patients with chronic 
      myelogenous leukemia are functional and carry the bcr-abl translocation.
PG  - 785-94
AB  - Leukemic bcr-abl positive dendritic cells (DCs) are likely to be present in vivo 
      in chronic myelogenous leukemia (CML) patients, but no data are available on 
      their functional qualities. We analyzed the circulating BDCA-1+ myeloid DC 
      compartment in 15 chronic phase CML patients. Phenotypic features of CML DCs were 
      comparable with that of normal DCs, except for the CD80 and CD40 antigens, 
      significantly under-represented in CML patients. Nonetheless, no differences were 
      found between normal samples and leukemic DCs in the allostimulatory ability, as 
      well as in the production of cytokines and polarization of T cell responses. CML 
      DCs were analyzed by fluorescence in situ hybridization (FISH) and found positive 
      for the bcr-abl translocation. However, when bcr-abl+ DCs were tested for their 
      ability to stimulate an autologous T-cell response in vitro, we could not detect 
      a specific recognition. We conclude that an apparently normal circulating DC 
      compartment carrying the Ph+ chromosome can be identified in CML patients; 
      however, these cells appear unable to trigger a protective anti-leukemic immune 
      response in autologous T cells.
FAU - Orsini, Enrica
AU  - Orsini E
AD  - Dipartimento di Biotecnologie Cellulari ed Ematologia, University "La Sapienza", 
      Via Benevento 6, 00161 Rome, Italy. orsini@bce.uniroma1.it
FAU - Calabrese, Elisabetta
AU  - Calabrese E
FAU - Maggio, Roberta
AU  - Maggio R
FAU - Pasquale, Alessia
AU  - Pasquale A
FAU - Nanni, Mauro
AU  - Nanni M
FAU - Trasarti, Stefania
AU  - Trasarti S
FAU - Tafuri, Agostino
AU  - Tafuri A
FAU - Guarini, Anna
AU  - Guarini A
FAU - Foa, Robert
AU  - Foa R
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20060309
PL  - England
TA  - Leuk Res
JT  - Leukemia research
JID - 7706787
RN  - 0 (Antigens, CD1)
RN  - 0 (Cytokines)
RN  - 0 (Lipopolysaccharides)
RN  - EC 2.7.10.2 (Fusion Proteins, bcr-abl)
SB  - IM
MH  - Antigens, CD1/drug effects/*immunology
MH  - CD4-Positive T-Lymphocytes/immunology
MH  - CD8-Positive T-Lymphocytes/immunology
MH  - Cytokines/immunology
MH  - Dendritic Cells/cytology/drug effects/*immunology
MH  - Fusion Proteins, bcr-abl/*genetics
MH  - Humans
MH  - Immunophenotyping
MH  - In Situ Hybridization, Fluorescence/methods
MH  - Leukemia, Myelogenous, Chronic, BCR-ABL Positive/diagnosis/*genetics
MH  - Lipopolysaccharides/pharmacology
MH  - Phenotype
MH  - Sensitivity and Specificity
MH  - Translocation, Genetic/*genetics
EDAT- 2006/03/11 09:00
MHDA- 2007/09/20 09:00
CRDT- 2006/03/11 09:00
PHST- 2005/03/03 00:00 [received]
PHST- 2005/05/22 00:00 [accepted]
PHST- 2006/03/11 09:00 [pubmed]
PHST- 2007/09/20 09:00 [medline]
PHST- 2006/03/11 09:00 [entrez]
AID - S0145-2126(05)00469-8 [pii]
AID - 10.1016/j.leukres.2005.11.028 [doi]
PST - ppublish
SO  - Leuk Res. 2006 Jul;30(7):785-94. doi: 10.1016/j.leukres.2005.11.028. Epub 2006 
      Mar 9.