PMID- 16528376
OWN - NLM
STAT- MEDLINE
DCOM- 20060710
LR  - 20230210
IS  - 0893-3952 (Print)
IS  - 0893-3952 (Linking)
VI  - 19
IP  - 5
DP  - 2006 May
TI  - Correlation of cytologic findings and chromosomal instability detected by 
      fluorescence in situ hybridization in breast fine-needle aspiration specimens 
      from women at high risk for breast cancer.
PG  - 622-9
AB  - Cytologic evaluation of ductal lavage or random periareolar fine-needle 
      aspiration (FNA) specimens has been proposed to improve risk stratification of 
      women at high risk for breast cancer. However, cytologic assessment of 
      morphologic changes is subjective. To assess the utility of fluorescence in situ 
      hybridization (FISH) in the categorization of breast lesions, we prospectively 
      evaluated 32 random periareolar FNA specimens from 27 women at high risk for 
      breast cancer. Cytologic specimens were prepared using the thin preparation 
      technique, and diagnoses were made on the basis of previously published criteria. 
      Specimens were also evaluated by FISH for chromosomes 1, 8, 11, and 17. Monosomy 
      was defined as the loss of one signal or both signals in >20% of cells, and 
      polysomy was defined as the presence of > or = 3 signals in >6% of cells. 
      Cytologic smears from seven invasive ductal carcinomas and nine benign breast 
      specimens from women at low risk for breast cancer were included for comparison. 
      In the high-risk group, cytologic findings were nonproliferative epithelium (NPE) 
      in 16 cases and hyperplasia in 16 cases. Chromosomal aberrations were detected in 
      11 (69%) of 16 NPE cases, 14 (89%) of 16 hyperplasia cases, seven (100%) of seven 
      carcinoma cases, and none of the low-risk cases. High-risk cases had 
      significantly more monosomy of chromosomes 1, 11, and 17 and polysomy of 
      chromosome 8 compared to low-risk cases and significantly less polysomy of 
      chromosomes 1, 8, 11, and 17 compared to patients with cancer. There were no 
      significant differences in monosomy or polysomy of individual chromosomes or a 
      combination of chromosomes between the NPE and hyperplasia groups. This study 
      shows that aberrations of chromosome number are common in high-risk women 
      irrespective of cytologic findings. Studies evaluating the association between 
      specific patterns of chromosomal polysomy and progression to malignancy may be 
      warranted.
FAU - Sneige, Nour
AU  - Sneige N
AD  - Division of Pathology and Laboratory Medicine, The University of Texas MD 
      Anderson Cancer Center, Houston, TX 77030, USA. nsneige@mdanderson.org
FAU - Liu, Baoshun
AU  - Liu B
FAU - Yin, Guosheng
AU  - Yin G
FAU - Gong, Yun
AU  - Gong Y
FAU - Arun, Banu K
AU  - Arun BK
LA  - eng
PT  - Journal Article
PL  - United States
TA  - Mod Pathol
JT  - Modern pathology : an official journal of the United States and Canadian Academy 
      of Pathology, Inc
JID - 8806605
SB  - IM
MH  - Adult
MH  - Aged
MH  - Biopsy, Fine-Needle
MH  - Breast/metabolism/*pathology
MH  - Breast Neoplasms/genetics/*pathology
MH  - *Chromosomal Instability
MH  - Chromosomes, Human, Pair 1/genetics
MH  - Chromosomes, Human, Pair 11/genetics
MH  - Chromosomes, Human, Pair 17/genetics
MH  - Chromosomes, Human, Pair 8/genetics
MH  - Cytodiagnosis/methods
MH  - Disease Progression
MH  - Female
MH  - Humans
MH  - Hyperplasia
MH  - In Situ Hybridization, Fluorescence/*methods
MH  - Middle Aged
MH  - Risk Factors
EDAT- 2006/03/11 09:00
MHDA- 2006/07/13 09:00
CRDT- 2006/03/11 09:00
PHST- 2006/03/11 09:00 [pubmed]
PHST- 2006/07/13 09:00 [medline]
PHST- 2006/03/11 09:00 [entrez]
AID - S0893-3952(22)03360-9 [pii]
AID - 10.1038/modpathol.3800571 [doi]
PST - ppublish
SO  - Mod Pathol. 2006 May;19(5):622-9. doi: 10.1038/modpathol.3800571.