PMID- 16529330
OWN - NLM
STAT- MEDLINE
DCOM- 20090602
LR  - 20161124
IS  - 1002-1892 (Print)
IS  - 1002-1892 (Linking)
VI  - 20
IP  - 2
DP  - 2006 Feb
TI  - [A study on in vitro force-vascularization and in vivo vascularization of porous 
      polylactic/glycolic acid copolymer scaffolds with internal network channels].
PG  - 181-4
AB  - OBJECTIVE: To study the influence of in vitro force-vascularization on in vivo 
      vascularization of porous polylactic glycolic acid copolymer (PLGA) scaffolds 
      with internal network channels (PPSINC). METHODS: After the in vitro 
      force-vascularization of PPSINCs covered with microvessel endothelial cells 
      (MVEC) of mice, they were divided into two groups: the force-vascularization 
      group (group A) and the control group with only PSINCs (group B). All the PPSINCs 
      were planted in the mesentery of 12 mice for 2 and 4 weeks, the PPSINCs were cut 
      out, the vascularization of PPSINCs was investigated by histology and 
      immunohistochemistry, and the vascularization area of the histologic section of 
      the PPSINCs was measured with the computer-assistant image analysis system. 
      RESULTS: After the in vitro force-vascularization of PPSINCs, the MVEC of the 
      mice sticking on the channel wall could be seen. After the scaffold was implanted 
      into the mice for 2 weeks, the vascularization area of the histologic section of 
      PPSINCs (VA) in group A (2 260.91 +/- 242.35 microm2) was compared with that in 
      group B (823.64 +/- 81.29 microm2), and the difference was significant in 
      statistics (P < 0.01). The VA for 4 weeks in group A (17 284.36 +/- 72.67 
      microm2) was compared with that in group B (17 041.14 +/- 81.51 microm2), and the 
      difference was not significant in statistics (P > 0.05). The area of the actin 
      positive-staining (AA) in the histologic section of PPSINCs for 2 weeks' 
      implantation in group A (565.22 +/- 60.58 microm2) was compared with that in 
      group B (205.91 +/- 16.25 microm2), and the difference was significant in 
      statistics (P < 0.01). After the implantation for 4 weeks, the VA in group A (4 
      321.09 +/- 19.82 microm2) was compared with group B (4 260.28 +/- 27.17 microm2), 
      and the difference was not significant in statistics (P > 0.05). CONCLUSION: The 
      PPSINC is a good simple scaffold model of vascularization. The in vitro 
      force-vascularization can increase the in vivo vascularization of PPSINCs in the 
      early stage.
FAU - Wu, Shihe
AU  - Wu S
AD  - The Institute of General Surgery, General Hospital of PLA, Beijing 100853, P R 
      China.
FAU - Xu, Yingxin
AU  - Xu Y
FAU - Song, Xuhua
AU  - Song X
FAU - Wang, Jinjing
AU  - Wang J
FAU - Li, Rong
AU  - Li R
LA  - chi
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - China
TA  - Zhongguo Xiu Fu Chong Jian Wai Ke Za Zhi
JT  - Zhongguo xiu fu chong jian wai ke za zhi = Zhongguo xiufu chongjian waike zazhi = 
      Chinese journal of reparative and reconstructive surgery
JID - 9425194
RN  - 0 (Glycolates)
RN  - 0 (Polyesters)
RN  - 0 (Polymers)
RN  - 0WT12SX38S (glycolic acid)
RN  - 33X04XA5AT (Lactic Acid)
RN  - 459TN2L5F5 (poly(lactide))
SB  - IM
MH  - Animals
MH  - Cell Culture Techniques
MH  - Cells, Cultured
MH  - Drug-Eluting Stents
MH  - Endothelial Cells/*cytology
MH  - Female
MH  - Glycolates
MH  - Lactic Acid
MH  - Mice
MH  - Microvessels
MH  - Polyesters
MH  - Polymers
MH  - Tissue Engineering/*methods
MH  - *Transplantation Conditioning
EDAT- 2006/03/15 09:00
MHDA- 2009/06/03 09:00
CRDT- 2006/03/15 09:00
PHST- 2006/03/15 09:00 [pubmed]
PHST- 2009/06/03 09:00 [medline]
PHST- 2006/03/15 09:00 [entrez]
PST - ppublish
SO  - Zhongguo Xiu Fu Chong Jian Wai Ke Za Zhi. 2006 Feb;20(2):181-4.